Cereal Research Communications
Volume/Issue:
Volume 37: Issue 3
Accumulation of H 2 O 2 and changes in activities of antioxidative enzymes and β -1,3-glucanase in barley and meadow fescue leaves attacked by Bipolaris sorokiniana
Authors:
A. Płażek Agricultural University of Krakow Department of Plant Physiology, Faculty of Agriculture Podłużna 3 30-239 Kraków Poland

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A. Skoczowski Polish Academy of Sciences The Franciszek Górski Institute of Plant Physiology Niezapominajek 21 30-239 Kraków Poland

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K. Hura Agricultural University of Krakow Department of Plant Physiology, Faculty of Agriculture Podłużna 3 30-239 Kraków Poland

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M. Libik Polish Academy of Sciences The Franciszek Górski Institute of Plant Physiology Niezapominajek 21 30-239 Kraków Poland

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B. Barna Hungarian Academy of Sciences Plant Protection Institute Herman O. utca 15 H-1022 Budapest Hungary

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Pages:
399–408
Online Publication Date:
08 Sep 2009
Publication Date:
01 Sep 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.37.2009.3.9
Keywords:
B. sorokiniana ; catalase; reactive oxygens; meadow fescue; peroxidase; resistance; SOD; spring barley
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The effects of Bipolaris sorokiniana inoculation on accumulation of hydrogen peroxide and changes in the activities of superoxide dismutase (SOD), catalase (CAT), non-specific peroxidase (PX) and β -1,3-glucanase enzymes in leaves of differentially resistant spring barley and meadow fescue were investigated in this study. Hydrogen peroxide accumulated slightly in inoculated barley leaves 24 hours after inoculation, and its accumulation after 48 hours of pathogenesis remained week around sites of infection. In leaves of fescue, which is more resistant to B. sorokiniana , accumulation of H 2 O 2 was faster and stronger in comparison to barley. A slight increase in SOD activity was observed only in infected fescue. Catalase activity in infected barely leaves decreased significantly 48 hours after inoculation, while in fescue-infected plants CAT activity, following a slight decrease, remained similar to the control values. PX activity was considerable lower in inoculated barley leaves after 6 hours, but at 24 and 48 hours after inoculation its activity increased significantly compared to the control. In inoculated fescue leaves activity of PX was higher at 6 and 24 hours compared to the control, while at 48 hours PX activity was strongly inhibited. β -1,3-Glucanase activity in inoculated barley plants did not differ from that of the control, while in infected fescue leaves it increased significantly 48 hours after inoculation. Based on the results, we suggest that the strong accumulation of H 2 O 2 , changes in antioxidant levels, together with the significant increase of β -1,3-Glucanase activity in infected fescue leaves, plays an important role in fescue’s greater resistance to B. sorokiniana .

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
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Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
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Springer Nature Switzerland AG
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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