Cereal Research Communications
Volume/Issue:
Volume 37: Issue 3
Identification of the Lr34/Yr18 rust resistance gene region in a Hungarian wheat breeding programme
Authors: Z. Wang 1 , L. Láng 2 , A. Uhrin 2 , O. Veisz 2 , S. Liu 1 and G. Vida 2
View More View Less
  • 1 Northwest A&F University College of Agronomy Yangling, Shaanxi 712100 China
  • 2 Agricultural Research Institute of the Hungarian Academy of Sciences H-2462 Martonvásár Hungary
DOI:
https://doi.org/10.1556/crc.37.2009.3.12
Page Count:
431–440
Publication Date:
01 Sep 2009
Online Publication Date:
08 Sep 2009
Article Category:
Research Article
Restricted access

The presence and frequency of the resistance gene complex Lr34/Yr18 was investigated in the wheat breeding programme of the Agricultural Research Institute, Martonvásár, Hungary. A total of 226 wheat cultivars and advanced lines from Hungary and other countries were tested with an STS marker, csLV34 , to understand the distribution of the Lr34/Yr18 resistance gene complex. A 150-bp PCR fragment was amplified in 64 wheat cultivars and lines with the resistance genes Lr34/Yr18 , while a 229-bp fragment was detected in 162 genotypes without Lr34/Yr18 . The genotypes with Lr34/Yr18 accounted for 28.3% of the wheat cultivars and advanced lines tested. Among the 128 varieties and breeding lines of Martonvásár origin tested, 34 carried the Lr34/Yr18 genes, with a frequency of 26.6%. The frequency of these genes was 30.6% in genotypes of other origin. The STS marker csLV34 could be used as an effective tool for the marker-assisted selection of Lr34/Yr18 genes in breeding wheat cultivars with durable rust resistance.

  • Bahl, P.N., Salimath, P.M., Mandal, A.K. 1997. Genetics, Cytogenetics and Breeding of Crop Plants. Oxford & IBH Publishing Co. PVT LTD, New Delhi and Calcutta, pp. 75–144.

    Mandal A.K. , '', in Genetics, Cytogenetics and Breeding of Crop Plants , (1997 ) -.

  • Bossolini, E., Krattinger, S.G., Keller, B. 2006. Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii . Theor. Appl. Genet. 113 :1049–1062.

    Keller B. , 'Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii ' (2006 ) 113 Theor. Appl. Genet. : 1049 -1062.

    • Search Google Scholar

  • Caldwell, R.M. 1968. Breeding for general and/or specific plant disease resistance. In: Finlay, K.W., Shephard, K.W. (eds). Proceedings of 3 rd International Wheat Genetics Symposium. Australian Academy of Sciences, Canberra, pp. 263–272.

    Caldwell R.M. , '', in Proceedings of 3rd International Wheat Genetics Symposium , (1968 ) -.

  • Chełkowski, J., Stȩpiem, Ł. 2001. Molecular markers for leaf rust resistance genes in wheat. J. Appl. Genet. 42 :117–126.

    Stȩpiem , 'Molecular markers for leaf rust resistance genes in wheat ' (2001 ) 42 J. Appl. Genet. : 117 -126.

    • Search Google Scholar

  • Dyck, P.L. 1994. Genetics of resistance to leaf rust and stem rust on wheat. Annual Wheat Newsletter 40 :63–64.

    Dyck P.L. , 'Genetics of resistance to leaf rust and stem rust on wheat ' (1994 ) 40 Annual Wheat Newsletter : 63 -64.

    • Search Google Scholar

  • Dyck, P.L. 1987. The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29 :467–469.

    Dyck P.L. , 'The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat ' (1987 ) 29 Genome : 467 -469.

    • Search Google Scholar

  • Dyck, P.L. 1991. Genetics of adult-plant leaf rust resistance in Chinese Spring and Sturdy wheats. Crop Sci. 31 :309–311.

    Dyck P.L. , 'Genetics of adult-plant leaf rust resistance in Chinese Spring and Sturdy wheats ' (1991 ) 31 Crop Sci. : 309 -311.

    • Search Google Scholar

  • Dyck, P.L., Samborski, D.J. 1982. The inheritance of resistance to Puccinia recondita in a group of common wheat cultivars. Can. J. Genet. Cytol. 24 :273–283.

    Samborski D.J. , 'The inheritance of resistance to Puccinia recondita in a group of common wheat cultivars ' (1982 ) 24 Can. J. Genet. Cytol. : 273 -283.

    • Search Google Scholar

  • Dyck, P. L., Samborski, D.J., Anderson, R.G. 1966. Inheritance of adult-plant leaf rust resistance derived from the common wheat varieties Exchange and Frontana. Can. J. Genet. Cytol. 8 :665–671.

    Anderson R.G. , 'Inheritance of adult-plant leaf rust resistance derived from the common wheat varieties Exchange and Frontana ' (1966 ) 8 Can. J. Genet. Cytol. : 665 -671.

    • Search Google Scholar

  • Kolmer, J.A., Singh, R.P., Garvin, D.F., Viccars, L., William, H.M., Huerta-Espino, J., Ogbonnaya, F.C., Raman, H., Orford, S., Bariana, H.S., Lagudah, E.S. 2008. Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm. Crop Sci. 48 :1841–1852.

    Lagudah E.S. , 'Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm ' (2008 ) 48 Crop Sci. : 1841 -1852.

    • Search Google Scholar

  • Lagudah, E.S., McFadden, H., Singh, R.P., Huerta, E.J., Bariana, H.S., Spielmeyer, W. 2006. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor. Appl. Genet. 114 :21–30.

    Spielmeyer W. , 'Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat ' (2006 ) 114 Theor. Appl. Genet. : 21 -30.

    • Search Google Scholar

  • Liang, D., Yang, F.P., He, Z.H., Yao, D.N., Xia, X.C. 2009. Characterization of Lr34/Yr18, Rht-B1b, Rht-D1b Genes in CIMMYT wheat cultivars and advanced lines using STS markers. Scientia Agricultura Sinica 42 :17–27.

    Xia X.C. , 'Characterization of Lr34/Yr18, Rht-B1b, Rht-D1b Genes in CIMMYT wheat cultivars and advanced lines using STS markers ' (2009 ) 42 Scientia Agricultura Sinica : 17 -27.

    • Search Google Scholar

  • Lillemo, M., Asalf, B., Singh, R.P., Huerta-Espino, J., Chen, X.M., He, Z.H., Bjørnstad, Å. 2008. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor. Appl. Genet. 116 :1155–1166.

    Bjørnstad , 'The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar ' (2008 ) 116 Theor. Appl. Genet. : 1155 -1166.

    • Search Google Scholar

  • McIntosh, R.A., Wellings, C.R., Park, R.F. 1995. Wheat Rusts–An Atlas of Resistance Genes. Kluwer Academic Publishers, Dordrecht, Boston, London, 200 pp.

    Park R.F. , '', in Wheat Rusts–An Atlas of Resistance Genes , (1995 ) -.

  • McIntosh, R.A., Yamazaki, Y., Dubcovsky, J., Rogers, J., Morris, C., Somers, D.J., Appels, R., Devos, K.M. 2008. Catalogue of gene symbols for wheat. In: Komugi–Integrated Wheat Science Database. http://www.shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp . Accessed 14 Jan 2009

  • Rubiales, D., Niks, R.E. 1995. Characterization of Lr34 , a major gene conferring non-hypersensitive resistance to wheat leaf rust. Plant Dis. 79 :1208–1212.

    Niks R.E. , 'Characterization of Lr34, a major gene conferring non-hypersensitive resistance to wheat leaf rust ' (1995 ) 79 Plant Dis. : 1208 -1212.

    • Search Google Scholar

  • Seah, S., Sivasithamparam, K., Karakousis, A., Lagudah, E.S. 1998. Cloning and characterisation of a family of disease resistance gene analogs from wheat and barley. Theor. Appl. Genet. 97 :937–945.

    Lagudah E.S. , 'Cloning and characterisation of a family of disease resistance gene analogs from wheat and barley ' (1998 ) 97 Theor. Appl. Genet. : 937 -945.

    • Search Google Scholar

  • Singh, R.P., Chen, W.Q., He, Z.H. 1999. Leaf rust resistance of spring, facultative, and winter wheat cultivars from China. Plant Dis. 83 :644–651.

    He Z.H. , 'Leaf rust resistance of spring, facultative, and winter wheat cultivars from China ' (1999 ) 83 Plant Dis. : 644 -651.

    • Search Google Scholar

  • Singh, R.P. 1992. Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology 82 :835–838.

    Singh R.P. , 'Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat ' (1992 ) 82 Phytopathology : 835 -838.

    • Search Google Scholar

  • Singh, R.P., Huerta-Espino, J., Rajaram, S. 2000. Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathologica et Entomologica Hungarica 35 :133–139.

    Rajaram S. , 'Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes ' (2000 ) 35 Acta Phytopathologica et Entomologica Hungarica : 133 -139.

    • Search Google Scholar

  • Singh, R.P., Rajaram, S. 1992. Genetics of adult-plant resistance to leaf rust in ‘Frontana’ and three CIMMYT wheats. Genome 35 :24–31.

    Rajaram S. , 'Genetics of adult-plant resistance to leaf rust in ‘Frontana’ and three CIMMYT wheats ' (1992 ) 35 Genome : 24 -31.

    • Search Google Scholar

  • Singh, R.P., Rajaram, S. 1994. Genetics of adult plant resistance to stripe rust in ten spring bread wheats. Euphytica 72 :1–7.

    Rajaram S. , 'Genetics of adult plant resistance to stripe rust in ten spring bread wheats ' (1994 ) 72 Euphytica : 1 -7.

    • Search Google Scholar

  • Spielmeyer, W., McIntosh, R.A., Kolmer, J., Lagudah, E.S. 2005. Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor. Appl. Genet. 111 :731–735.

    Lagudah E.S. , 'Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat ' (2005 ) 111 Theor. Appl. Genet. : 731 -735.

    • Search Google Scholar

  • Spielmeyer, W., Singh, R.P., McFadden, H., Wellings, C.R., Huerta-Espino, J., Kong, X., Appels, R., Lagudah, E.S. 2008. Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective against multiple pathogens in wheat. Theor. Appl. Genet. 116 :481–490.

    Lagudah E.S. , 'Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective against multiple pathogens in wheat ' (2008 ) 116 Theor. Appl. Genet. : 481 -490.

    • Search Google Scholar

  • Stubbs, R.W., Prescott, E.E., Saari, E.E., Dubin, H.J. 1986. Cereal Disease Methodology Manual. CIMMYT, Mexico, 46 pp.

    Dubin H.J. , '', in Cereal Disease Methodology Manual , (1986 ) -.

  • Yoshimura, S., Yoshimura, A., Iwata, N., McCouch, S.R., Abenes, M.L., Baraoidan, M.R., Mew, T.W., Nelson, R. 1995. Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. Mol. Breed. 1 :375–387.

    Nelson R. , 'Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers ' (1995 ) 1 Mol. Breed. : 375 -387.

    • Search Google Scholar

  • Zadoks, J.C., Chang, T.T., Konzak, C.F. 1974. A decimal code for the growth stages of cereals. Weed Research 14 :415–421.

    Konzak C.F. , 'A decimal code for the growth stages of cereals ' (1974 ) 14 Weed Research : 415 -421.

    • Search Google Scholar
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170
Keywords:
Triticum aestivum L.; leaf rust; marker-assisted selection; Lr34/Yr18