Cereal Research Communications
Volume/Issue:
Volume 37: Issue 4
Evaluation of genetic diversity among bread wheat varieties and landraces of Pakistan by SSR markers
Authors: N. Iqbal 1 , A. Tabasum 1 , H. Sayed 1 , and A. Hameed 1
View More View Less
  • 1 Nuclear Institute for Agriculture and Biology (NIAB) Marker Assisted Breeding Group PO Box 128 Jhang Road Faisalabad Pakistan
DOI:
https://doi.org/10.1556/crc.37.2009.4.2
Page Count:
489–498
Publication Date:
01 Dec 2009
Online Publication Date:
23 Dec 2009
Article Category:
Research Article
Restricted access

Genetic diversity of 48 Pakistani wheat varieties and 12 landraces was assessed, loss of genetic diversity in bread wheat during the change from traditional landraces (LVs) to modern breeding varieties was examined, and recent trends of national wheat breeding programmes were identified. A total of 29 SSR markers, representing at least one marker from each chromosome of wheat, were used to analyze the genetic diversity. A total of 80 alleles were generated by the 29 loci with an average of 2.76 alleles per marker. A significant loss of genetic diversity was observed from LVs to elite cultivated varieties. Average genetic similarity between landraces was 61% while varieties released after 1990 showed 73% similarity. Range of genetic distance observed between all possible pairs was from 1.41 to 4.90. It was also observed that most of the varieties released from one source showed comparatively lower diversity indicating the utilization of common elite breeding material or interbreeding of released varieties.

  • Anderson, J.A., Churchill, G.A., Autrique, J.E., Sorrells, M.E., Tanksley, S.D. 1993. Optimizing parental selection for genetic linkage maps. Genome 36 :181–186.

    Tanksley S.D. , 'Optimizing parental selection for genetic linkage maps ' (1993 ) 36 Genome : 181 -186.

    • Search Google Scholar

  • Barrett, B.A., Kidwell, K.K. 1998. AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest. Crop Sci. 38 :1261–1271.

    Kidwell K.K. , 'AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest ' (1998 ) 38 Crop Sci : 1261 -1271.

    • Search Google Scholar

  • Börner, A., Chebotar, S., Korzun, V. 2000. Molecular characterization of the genetic integrity of wheat ( Triticum aestivum L.) germplasm after long-term maintenance. Theor. Appl. Genet. 100 :494–497.

    Korzun V. , 'Molecular characterization of the genetic integrity of wheat (Triticum aestivum L.) germplasm after long-term maintenance ' (2000 ) 100 Theor. Appl. Genet. : 494 -497.

    • Search Google Scholar

  • Burkhamer, R.L., Lanning, S.P., Martens, R.J., Martin, J.M., Talbert, L.E. 1998. Predicting progeny variance from parental divergence in hard red spring wheat. Crop Sci. 38 :243–248.

    Talbert L.E. , 'Predicting progeny variance from parental divergence in hard red spring wheat ' (1998 ) 38 Crop Sci : 243 -248.

    • Search Google Scholar

  • Chen, H.B., Martin, J.M., Lavin, M., Talbert, L.E. 1994. Genetic diversity in hard red spring wheat based on sequence-tagged-site PCR markers. Crop Sci. 34 :1628–1632.

    Talbert L.E. , 'Genetic diversity in hard red spring wheat based on sequence-tagged-site PCR markers ' (1994 ) 34 Crop Sci : 1628 -1632.

    • Search Google Scholar

  • Devos, K.M., Bryan, G.J., Collins, A.J., Gale, M.D. 1995. Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor. Appl. Genet. 90 :247–252.

    Gale M.D. , 'Application of two microsatellite sequences in wheat storage proteins as molecular markers ' (1995 ) 90 Theor. Appl. Genet. : 247 -252.

    • Search Google Scholar

  • Huang, X. Q., Börner, A., Röder, M. S., Ganal, M. W. 2002. Assessing genetic diversity of wheat ( Triticum aestivum L.) germplasm using microsatellite markers. Theor. Appl. Genet. 105 :699–707.

    Ganal M. W. , 'Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers ' (2002 ) 105 Theor. Appl. Genet. : 699 -707.

    • Search Google Scholar

  • Iqbal, N., Firdissa, E., Khlestkina, K., Weidner, A., Röder, M.S., Börner, A. 2007. The use of simple sequence repeat (SSR) markers to identify and map alien segments carrying genes for effective resistance to leaf rust in bread wheat. Plant Genet. Resour. 5 :100–103.

    Börner A. , 'The use of simple sequence repeat (SSR) markers to identify and map alien segments carrying genes for effective resistance to leaf rust in bread wheat ' (2007 ) 5 Plant Genet. Resour. : 100 -103.

    • Search Google Scholar

  • Joshi, C.P., Nguyen, H.T. 1993. RAPD (random amplied polymorphic DNA) analysis based intervarietal genetic relationships among hexaploid wheats. Plant Sci. 93 :95–103.

    Nguyen H.T. , 'RAPD (random amplied polymorphic DNA) analysis based intervarietal genetic relationships among hexaploid wheats ' (1993 ) 93 Plant Sci. : 95 -103.

    • Search Google Scholar

  • Kim, H.S., Ward, R.W. 2000. Patterns of RFLP-based genetic diversity in germplasm pools of common wheat with different geographical or breeding program origins. Euphytica 115 :197–208.

    Ward R.W. , 'Patterns of RFLP-based genetic diversity in germplasm pools of common wheat with different geographical or breeding program origins ' (2000 ) 115 Euphytica : 197 -208.

    • Search Google Scholar

  • Landjeva, S., Korzun V., Ganeva, G. 2006. Evaluation of genetic diversity among Bulgarian winter wheat ( Triticum aestivum L.) varieties during the period 1925–2003 using microsatellites. Genet. Resour. Crop Evol. 53 :1605–1614.

    Ganeva G. , 'Evaluation of genetic diversity among Bulgarian winter wheat (Triticum aestivum L.) varieties during the period 1925–2003 using microsatellites ' (2006 ) 53 Genet. Resour. Crop Evol. : 1605 -1614.

    • Search Google Scholar

  • Leonova, I., Börner, A., Budashkina, E., Unger, N.O., Röder, M., Salina, E. 2004. Identification of microsatellite markers for a leaf rust resistance gene introgressed into common wheat from Triticum timopheevii . Plant Breeding 123 :93–95.

    Salina E. , 'Identification of microsatellite markers for a leaf rust resistance gene introgressed into common wheat from Triticum timopheevii ' (2004 ) 123 Plant Breeding : 93 -95.

    • Search Google Scholar

  • Masmoudi, K., Rebai, A., Ellouz, R. 2006. AFLP and SSR fingerprinting to evaluate genetic diversity among bread wheat cultivars in Tunisia. Cereal Res. Commun. 34 :871–878.

    Ellouz R. , 'AFLP and SSR fingerprinting to evaluate genetic diversity among bread wheat cultivars in Tunisia ' (2006 ) 34 Cereal Res. Commun. : 871 -878.

    • Search Google Scholar

  • Nagaoka, T., Ogihara, Y. 1997. Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor. Appl. Genet. 94 :597–602.

    Ogihara Y. , 'Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers ' (1997 ) 94 Theor. Appl. Genet. : 597 -602.

    • Search Google Scholar

  • Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70 :3321–3323.

    Nei M. , 'Analysis of gene diversity in subdivided populations ' (1973 ) 70 Proc. Natl. Acad. Sci. USA : 3321 -3323.

    • Search Google Scholar

  • Niwa, K., Suzuki, H., Tominaga, T., Nasim, S., Anwar, R., Ogawa, M., Furuta, Y. 2008. Evaluation of genetic variation in high molecular weight glutenin subunits of seed storage protein using landraces of common wheat from Pakistan. Cereal Res. Commun. 36 :327–332.

    Furuta Y. , 'Evaluation of genetic variation in high molecular weight glutenin subunits of seed storage protein using landraces of common wheat from Pakistan ' (2008 ) 36 Cereal Res. Commun. : 327 -332.

    • Search Google Scholar

  • Plaschke, J., Ganal, M.W., Röder, M.S. 1995. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 91 :1001–1007.

    Röder M.S. , 'Detection of genetic diversity in closely related bread wheat using microsatellite markers ' (1995 ) 91 Theor. Appl. Genet. : 1001 -1007.

    • Search Google Scholar

  • Prasad, M., Varshney, R.K., Roy, J.K., Balyan, H.S., Gupta, P.K. 2000. The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor. Appl. Genet. 100 :584–592.

    Gupta P.K. , 'The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat ' (2000 ) 100 Theor. Appl. Genet. : 584 -592.

    • Search Google Scholar

  • Röder, M.S., Korzun, V., Gill, B.S., Ganal, M.W. 1998a. The physical mapping of microsatellite markers in wheat. Genome 41 :278–283.

    Ganal M.W. , 'The physical mapping of microsatellite markers in wheat ' (1998 ) 41 Genome : 278 -283.

    • Search Google Scholar

  • Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998b. A microsatellite map of wheat. Genetics 149 :2007–2023.

    Ganal M.W. , 'A microsatellite map of wheat ' (1998 ) 149 Genetics : 2007 -2023.

  • Röder, M.S., Plaschke, J., Konig, S.U., Börner, A., Sorrels, M.E., Tanksley, S.D., Ganal, M.W. 1995. Abundance, variability and chromosomal location of microsatellites in wheat. Mol. Gen. Genet. 246 :327–333.

    Ganal M.W. , 'Abundance, variability and chromosomal location of microsatellites in wheat ' (1995 ) 246 Mol. Gen. Genet. : 327 -333.

    • Search Google Scholar

  • Röder, M.S., Wendehake, K., Korzun, V., Bredemeijer, G., Laborie, D., Bertrand, L., Isaac, P., Rendell, S., Jackson, J., Cooke, R.J., Vosmann, B., Ganal, M.W. 2002. Construction and analysis of a microsatellite-based database of European wheat cultivars. Theor. Appl. Genet. 106 :67–73.

    Ganal M.W. , 'Construction and analysis of a microsatellite-based database of European wheat cultivars ' (2002 ) 106 Theor. Appl. Genet. : 67 -73.

    • Search Google Scholar

  • Saghai Maroof, M.A., Biyashev, R.M., Yang, G.P., Zhang, Q., Allard, R.W. 1994 Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proc. Natl. Acad. Sci. USA 91 :5466–5470.

    Allard R.W. , 'Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics ' (1994 ) 91 Proc. Natl. Acad. Sci. USA : 5466 -5470.

    • Search Google Scholar

  • Siedler, H., Messmer, M.M., Schachermayr, G.M., Winzeler, H., Winzeler, M., Keller, B. 1994. Genetic diversity in European wheat and spelt breeding material based on RFLP data. Theor. Appl. Genet. 88 :994–1003.

    Keller B. , 'Genetic diversity in European wheat and spelt breeding material based on RFLP data ' (1994 ) 88 Theor. Appl. Genet. : 994 -1003.

    • Search Google Scholar

  • Stachel, M., Lelley, T., Grausgruber, H., Vollmann, T. 2000. Application of microsatellites in wheat ( Triticum aestivum L.) for studying genetic differentiation caused by selection for adaptation and use. Theor. Appl. Genet. 100 :242–248.

    Vollmann T. , 'Application of microsatellites in wheat (Triticum aestivum L.) for studying genetic differentiation caused by selection for adaptation and use ' (2000 ) 100 Theor. Appl. Genet. : 242 -248.

    • Search Google Scholar

  • Tautz, D., Renz, M. 1984. Simple sequence are ubiquitous repetitive components of eukaryotic genomes. Nucl. Acid Res. 12 :4127–4138.

    Renz M. , 'Simple sequence are ubiquitous repetitive components of eukaryotic genomes ' (1984 ) 12 Nucl. Acid Res. : 4127 -4138.

    • Search Google Scholar

  • Wu, K.S., Tanksley, S.D. 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol. Gen. Genet. 241 :225–235.

    Tanksley S.D. , 'Abundance, polymorphism and genetic mapping of microsatellites in rice ' (1993 ) 241 Mol. Gen. Genet. : 225 -235.

    • Search Google Scholar
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170
Keywords:
genetic diversity; microsatellite markers; SSR; wheat

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2020 4 0 0
Sep 2020 5 0 0
Oct 2020 11 0 0
Nov 2020 2 3 0
Dec 2020 2 0 0
Jan 2021 0 1 2
Feb 2021 0 0 0