Cereal Research Communications
Volume/Issue:
Volume 37: Issue 4
Determination of mycotoxin concentration by ELISA and near-infrared spectroscopy in Fusarium-inoculated maize
Authors:
C. Bolduan University of Hohenheim Institute of Plant Breeding, Seed Science and Population Genetics Fruwirthstr. 21 70593 Stuttgart Germany

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J. Montes University of Hohenheim Institute of Plant Breeding, Seed Science and Population Genetics Fruwirthstr. 21 70593 Stuttgart Germany

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B. Dhillon University of Hohenheim Institute of Plant Breeding, Seed Science and Population Genetics Fruwirthstr. 21 70593 Stuttgart Germany

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V. Mirdita University of Hohenheim Institute of Plant Breeding, Seed Science and Population Genetics Fruwirthstr. 21 70593 Stuttgart Germany

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A. Melchinger University of Hohenheim Institute of Plant Breeding, Seed Science and Population Genetics Fruwirthstr. 21 70593 Stuttgart Germany

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Pages:
521–529
Online Publication Date:
23 Dec 2009
Publication Date:
01 Dec 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.37.2009.4.5
Keywords:
ELISA; near-infrared spectroscopy; mycotoxins; Fusarium graminearum ; Fusarium verticillioides ; Zea mays ; resistance breeding
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Ear rots of maize caused by Fusarium spp. reduce grain yield and produce mycotoxins, which are harmful to humans and animals. To breed maize cultivars resistant to Fusarium spp., reliable large-scale phenotyping is essential. Our objectives were to (i) examine the precision of the ELISA method for determination of important mycotoxins, namely deoxynivalenol (DON) and fumonisins (FUM), (ii) evaluate the potential of near-infrared reflectance spectroscopy (NIRS) to estimate concentrations of DON and FUM in grain produced in inoculated maize plants, and (iii) compare the efficiency of ELISA, NIRS, and visual rating of disease severity for estimation of mycotoxin concentrations. Insignificant variation was observed between duplicate evaluations of DON and FUM by ELISA, showing the high repeatability of this method. DON and FUM determinations by ELISA were more closely correlated with mycotoxin concentrations predicted through NIRS than with visual rating of disease severity. For the prediction of DON, NIRS had very high magnitude of the coefficients of determination of calibration and cross validation (R 2 = 0.90–0.88). Thus, NIRS has a promising potential to predict DON concentration in grain samples of inoculated maize genotypes evaluated in resistance breeding programs.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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