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  • 1 University of Hohenheim Institute of Plant Breeding, Seed Science and Population Genetics Fruwirthstr. 21 70593 Stuttgart Germany
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Ear rots of maize caused by Fusarium spp. reduce grain yield and produce mycotoxins, which are harmful to humans and animals. To breed maize cultivars resistant to Fusarium spp., reliable large-scale phenotyping is essential. Our objectives were to (i) examine the precision of the ELISA method for determination of important mycotoxins, namely deoxynivalenol (DON) and fumonisins (FUM), (ii) evaluate the potential of near-infrared reflectance spectroscopy (NIRS) to estimate concentrations of DON and FUM in grain produced in inoculated maize plants, and (iii) compare the efficiency of ELISA, NIRS, and visual rating of disease severity for estimation of mycotoxin concentrations. Insignificant variation was observed between duplicate evaluations of DON and FUM by ELISA, showing the high repeatability of this method. DON and FUM determinations by ELISA were more closely correlated with mycotoxin concentrations predicted through NIRS than with visual rating of disease severity. For the prediction of DON, NIRS had very high magnitude of the coefficients of determination of calibration and cross validation (R 2 = 0.90–0.88). Thus, NIRS has a promising potential to predict DON concentration in grain samples of inoculated maize genotypes evaluated in resistance breeding programs.

  • Berardo, N., Pisacane, V., Battilani, P., Scandolara, A., Pietri, A., Marocco, A. 2005. Rapid detection of kernel rots and mycotoxins in maize by near-infrared reflectance spectroscopy. J. Agricul. and Food Chem. 53 :8128–8134.

    Marocco A. , 'Rapid detection of kernel rots and mycotoxins in maize by near-infrared reflectance spectroscopy ' (2005 ) 53 J. Agricul. and Food Chem. : 8128 -8134.

    • Search Google Scholar
  • Bolduan, C., Miedaner, T., Schipprack, W., Dhillon, B.S., Melchinger, A.E. 2009. Genetic Variation for Resistance to Ear Rot and Mycotoxin Contamination in Early European Maize Inbred Lines. Crop Sci. In press.

  • Josephs, R.D., Schuhmacher, R., Krska, R. 2001. International interlaboratory study for the determination of the Fusarium mycotoxins zearalenone and deoxynivalenol in agricultural commodities. Food Additives and Contaminants 18 :417–430.

    Krska R. , 'International interlaboratory study for the determination of the Fusarium mycotoxins zearalenone and deoxynivalenol in agricultural commodities ' (2001 ) 18 Food Additives and Contaminants : 417 -430.

    • Search Google Scholar
  • Kos, G., Lohninger, H., Krska, R. 2002. Fourier transform mid-infrared spectroscopy with attenuated total reflection (FT-IR/ATR) as a tool for the detection of Fusarium fungi on maize. Vibrational Spectroscopy 29 :115–119.

    Krska R. , 'Fourier transform mid-infrared spectroscopy with attenuated total reflection (FT-IR/ATR) as a tool for the detection of Fusarium fungi on maize ' (2002 ) 29 Vibrational Spectroscopy : 115 -119.

    • Search Google Scholar
  • Kos, G., Krska, R., Lohninger, H., Griffiths, P.R. 2004. A comparative study of mid-infrared diffuse reflection (DR) and attenuated total reflection (ATR) spectroscopy for the detection of fungal infection on RWA2-corn. Analytical and Bioanalytical Chem. 378 :159–166.

    Griffiths P.R. , 'A comparative study of mid-infrared diffuse reflection (DR) and attenuated total reflection (ATR) spectroscopy for the detection of fungal infection on RWA2-corn ' (2004 ) 378 Analytical and Bioanalytical Chem. : 159 -166.

    • Search Google Scholar
  • Krska, R., Welzig, E., Boudra, H. 2007. Analysis of Fusarium toxins in feed. Animal Feed Sci. and Technol. 137 :241–264.

    Boudra H. , 'Analysis of Fusarium toxins in feed ' (2007 ) 137 Animal Feed Sci. and Technol. : 241 -264.

    • Search Google Scholar
  • Logrieco, A., Mulé, G., Moretti, A., Bottalico, A. 2002. Toxigenic Fusarium species and mycotoxins associated with maize ear rot in Europe. Europ. J. Plant Path. 108 :597–609.

    Bottalico A. , 'Toxigenic Fusarium species and mycotoxins associated with maize ear rot in Europe ' (2002 ) 108 Europ. J. Plant Path. : 597 -609.

    • Search Google Scholar
  • Miller, J.D. 2008. Mycotoxins in small grains and maize: Old problems, new challenges. Food Additives and Contaminants 25 :219–230.

    Miller J.D. , 'Mycotoxins in small grains and maize: Old problems, new challenges ' (2008 ) 25 Food Additives and Contaminants : 219 -230.

    • Search Google Scholar
  • Munkvold, G.P., Desjardins, A.E. 1997. Fumonisins in maize. Can we reduce their Occurrence? Plant Disease 81 :556–565.

    Desjardins A.E. , 'Fumonisins in maize. Can we reduce their Occurrence? ' (1997 ) 81 Plant Disease : 556 -565.

    • Search Google Scholar
  • Reid, L.M., Hamilton, R.I., Mather, D.E. 1996a. Screening maize for resistance to gibberella ear rot. Agriculture and Agri-Food Canada Technical 1996-195E, p. 31.

    Mather D.E. , 'Screening maize for resistance to gibberella ear rot ' (1996 ) 1996-195E Agriculture and Agri-Food Canada Technical : 31 -.

    • Search Google Scholar
  • Reid, L.M., Stewart, D.W., Hamilton, R.I. 1996b. A 4-year study of the association between gibberella ear rot severity and deoxynivalenol concentration. J. Phytopath. 144 :431–436.

    Hamilton R.I. , 'A 4-year study of the association between gibberella ear rot severity and deoxynivalenol concentration ' (1996 ) 144 J. Phytopath. : 431 -436.

    • Search Google Scholar
  • Reid, L.M., Sinha, R.C. 1998. Maize maturity and the development of gibberella ear rot symptoms and deoxynivalenol after inoculation. Europ. J. Plant Path. 104 :147–154.

    Sinha R.C. , 'Maize maturity and the development of gibberella ear rot symptoms and deoxynivalenol after inoculation ' (1998 ) 104 Europ. J. Plant Path. : 147 -154.

    • Search Google Scholar
  • Robertson, L.A., Kleinschmidt, C.E., White, D.G., Payne, G.A., Maragos, C.M., Holland, J.B. 2006. Heritabilities and correlations of fusarium ear rot resistance and fumonisin contamination resistance in two maize populations. Crop Sci. 46 :353–361.

    Holland J.B. , 'Heritabilities and correlations of fusarium ear rot resistance and fumonisin contamination resistance in two maize populations ' (2006 ) 46 Crop Sci. : 353 -361.

    • Search Google Scholar

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Cereal Research Communications
Language English
Size B5
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
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Address
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Springer Nature Switzerland AG
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ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)