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  • 1 Hungarian Academy of Sciences Plant Protection Institute P.O. Box 102 H-1525 Budapest Hungary
  • 2 Agricultural Biotechnology Center Szent-Györgyi A. út 4 H-2100 Gödöllő Hungary
  • 3 Corvinus University of Budapest Department of Plant Pathology Ménesi út 44 H-1118 Budapest Hungary
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Barley-infecting isolates of Wheat dwarf virus (WDV) were collected in the field in the vicinity of the cities Dunakiliti, Heves and Siófok, in Hungary. Viral genomic DNA was amplified by the rolling circle amplification technique, digested with Hind III, cloned into pBSK+ plasmid and sequenced. The clones were of the same size and showed above 99% identity to each other. Based on DNA sequences WDV-D01, WDV-H1 and WDV-H07 isolates showed high identity (94–99%) to isolates of WDV barley strain and Barley dwarf virus and lower identity to Oat dwarf virus (71% identity) and WDV wheat strains (85% identity).

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