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  • 1 Gebze Institute of Technology Faculty of Science, Department of Biology Cayirova Campus Gebze, Kocaeli Turkey
  • 2 Marmara University Faculty of Science and Letters, Department of Biology 34722 Kadikoy, Istanbul Turkey
  • 3 The Scientific and Technological Research Council of Turkey (TUBITAK) Marmara Research Center (MRC), Genetic Engineering and Biotechnology Institute (GEBI) P.O Box 21 41470 Gebze, Kocaeli Turkey
  • 4 Sabancé University Faculty of Engineering and Natural Sciences, Biological Sciences & Bioengineering Orhanlé — Tuzla 34956 Istanbul Turkey
  • 5 Central Research Institute of Field Crops P.O. Box 226 Lodumlu, Ankara Turkey
  • 6 Anatolian Agricultural Research Institute P.O. Box 17 26001 Eskişehir Turkey
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Bulk segregant analysis (BSA) was used to identify molecular markers associated with yellow rust disease resistance in wheat ( Triticum aestivum L.). DNAs isolated from the selected yellow rust tolerant and susceptible F 2 individuals derived from a cross between yellow rust resistant and susceptible wheat genotypes were used to established a “tolerant“ and a “susceptible“ DNA pool. The BSA was then performed on these DNA pools using 230 markers that were previously mapped onto the individual wheat chromosomes. One of the SSR markers (Xgwm382) located on chromosome group 2 (A, B, D genomes) was present in the resistant parent and the resistant bulk but not in the susceptible parent and the susceptible bulk, suggesting that this marker is linked to a yellow rust resistance gene. The presence of Xgwm382 was also tested in 108 additional wheat genotypes differing in yellow rust resistance. This analysis showed that 81% of the wheat genotypes known to be yellow rust resistant had the Xgwm382 marker, further suggesting that the presence of this marker correlates with yellow rust resistance in diverse wheat germplasm. Therefore, Xgwm382 could be useful for marker assisted selection of yellow rust resistances genotypes in wheat breeding programs.

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