Cereal Research Communications
Volume/Issue:
Volume 38: Issue 2
Microsatellite mapping of a leaf rust resistance gene transferred to common wheat from Triticum timopheevii
Authors: I. Leonova 1 , E. Budashkina 1 , K. Flath 2 , A. Weidner 3 , A. Börner 3 , and M. Röder 3
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  • 1 Russian Academy of Sciences Institute of Cytology and Genetics Lavrentieva ave. 10 Novosibirsk 630090 Russia
  • 2 Institute for Plant Protection in Field Crops and Grassland Federal Research Centre for Cultivated Plants — Julius Kuehn Institute Stahnsdorfer Damm 81 D-14532 Kleinmachnow Germany
  • 3 Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Corrensstrasse, 3 D-06466 Gatersleben Germany
DOI:
https://doi.org/10.1556/crc.38.2010.2.7
Page Count:
211–219
Publication Date:
01 Jun 2010
Online Publication Date:
25 May 2010
Article Category:
Research Article
Restricted access

A leaf rust resistance gene transferred from the tetraploid wheat Triticum timopheevii (Zhuk.) Zhuk. (genomic composition: A t A t GG) into common wheat Triticum aestivum L. conditioned resistance at the seedling and adult plant stages in the introgression line ‘line 842-2’. To determine chromosome location and to map the resistance gene an F 2 population from a cross between ‘line 842-2’ and susceptible wheat cultivar ‘Skala’ was developed and screened against leaf rust pathotype 77 ( Puccinia triticina Erikss.). Microsatellite markers detected introgressions of the T. timopheevii genome on chromosomes 1A, 2A, 2B, 5B and 6B of ‘line 842-2’. Linkage analysis revealed an association between leaf rust resistance and microsatellite markers located on chromosome 5B. The markers Xgwm880 and Xgwm1257 were closely linked to the resistance gene with genetic distances of 7.7 cM and 10.4 cM, respectively. Infection type tests with three leaf rust isolates resulted in different patterns of infection types of ‘line 842-2’ and ‘Thatcher’ near-isogenic line with the Lr18 gene on chromosome 5B. The data corroborated the hypothesis of the diversity of the resistance coming from T. timopheevii . The resistance gene of the introgression ‘line 842-2’ seems to be different than Lr18 and therefore it was designated LrTt2 .

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170
Keywords:
chromosome localization; introgression lines; leaf rust resistance; microsatellite markers; Puccinia triticina ; Triticum timopheevii

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