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  • 1 Russian Academy of Sciences Institute of Cytology and Genetics Lavrentieva ave. 10 Novosibirsk 630090 Russia
  • 2 Institute for Plant Protection in Field Crops and Grassland Federal Research Centre for Cultivated Plants — Julius Kuehn Institute Stahnsdorfer Damm 81 D-14532 Kleinmachnow Germany
  • 3 Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Corrensstrasse, 3 D-06466 Gatersleben Germany
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A leaf rust resistance gene transferred from the tetraploid wheat Triticum timopheevii (Zhuk.) Zhuk. (genomic composition: A t A t GG) into common wheat Triticum aestivum L. conditioned resistance at the seedling and adult plant stages in the introgression line ‘line 842-2’. To determine chromosome location and to map the resistance gene an F 2 population from a cross between ‘line 842-2’ and susceptible wheat cultivar ‘Skala’ was developed and screened against leaf rust pathotype 77 ( Puccinia triticina Erikss.). Microsatellite markers detected introgressions of the T. timopheevii genome on chromosomes 1A, 2A, 2B, 5B and 6B of ‘line 842-2’. Linkage analysis revealed an association between leaf rust resistance and microsatellite markers located on chromosome 5B. The markers Xgwm880 and Xgwm1257 were closely linked to the resistance gene with genetic distances of 7.7 cM and 10.4 cM, respectively. Infection type tests with three leaf rust isolates resulted in different patterns of infection types of ‘line 842-2’ and ‘Thatcher’ near-isogenic line with the Lr18 gene on chromosome 5B. The data corroborated the hypothesis of the diversity of the resistance coming from T. timopheevii . The resistance gene of the introgression ‘line 842-2’ seems to be different than Lr18 and therefore it was designated LrTt2 .

  • Badaeva, E.D., Budashkina, E.B., Badaev, N.S., Kalinina, N.P. 1991. General features of chromosome substitutions in Triticum aestivum × T. timopheevii hybrids. Theor. Appl. Genet. 82 :227–232.

    Kalinina N.P. , 'General features of chromosome substitutions in Triticum aestivum × T. timopheevii hybrids ' (1991 ) 82 Theor. Appl. Genet. : 227 -232.

    • Search Google Scholar
  • Badaeva, E.D., Prokofieva, Z.D., Bilinskaya, E.N., Obolenkova, L.A., Solomatin, D.A., Zelenin, A.V., Pukhalskyi, V.A. 2000. Cytogenetic analysis of hybrids resistant to leaf rust and powdery mildew obtained by crossing common wheat ( Triticum aestivum L.) with wheats of the Timopheevi group. Rus. J. Genet. 36 :1401–1410.

    Pukhalskyi V.A. , 'Cytogenetic analysis of hybrids resistant to leaf rust and powdery mildew obtained by crossing common wheat (Triticum aestivum L.) with wheats of the Timopheevi group ' (2000 ) 36 Rus. J. Genet. : 1401 -1410.

    • Search Google Scholar
  • Brown-Guedira, G.L., Singh, S., Fritz, A.K. 2003. Performance and mapping of leaf rust resistance transferred to wheat from Triticum timopheevii subsp. armeniacum . Phytopathology 93 :784–789.

    Fritz A.K. , 'Performance and mapping of leaf rust resistance transferred to wheat from Triticum timopheevii subsp. armeniacum ' (2003 ) 93 Phytopathology : 784 -789.

    • Search Google Scholar
  • Budashkina, E.B., Kalinina, N.P. 2001. Development and genetic analysis of common wheat introgressive lines resistant to leaf rust. Acta Phytopathol. Entomol. 36 :61–65.

    Kalinina N.P. , 'Development and genetic analysis of common wheat introgressive lines resistant to leaf rust ' (2001 ) 36 Acta Phytopathol. Entomol. : 61 -65.

    • Search Google Scholar
  • Collard, B.C.Y., Jahufer, M.Z.Z., Pang, E.C.K. 2005. An introduction to markers, quantitative trait loci (QTL), mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142 :169–196.

    Pang E.C.K. , 'An introduction to markers, quantitative trait loci (QTL), mapping and marker-assisted selection for crop improvement: the basic concepts ' (2005 ) 142 Euphytica : 169 -196.

    • Search Google Scholar
  • Gilmour, J. 1973. Octal notation for designating of physiologic races of plant pathogens. Nature 242 :620.

    Gilmour J. , 'Octal notation for designating of physiologic races of plant pathogens ' (1973 ) 242 Nature : 620 -.

    • Search Google Scholar
  • Järve, K., Peusha, H.O., Tsymbalova, J., Tamm, S., Devos, K.M., Enno, T.M. 2000. Chromosomal location of a Triticum timopheevii -derived powdery mildew resistance gene transferred to common wheat. Genome 43 :377–381.

    Enno T.M. , 'Chromosomal location of a Triticum timopheevii-derived powdery mildew resistance gene transferred to common wheat ' (2000 ) 43 Genome : 377 -381.

    • Search Google Scholar
  • Khlestkina, E.K., Than, M.H.M., Pestsova, E.G., Röder, M.S., Malyshev, S.V., Korzun, V., Börner, A. 2004. Mapping of new 99 new microsatellite loci in rye ( Secale cereale L.) including 39 expressed sequence tags. Theor. Appl. Genet. 109 :725–732.

    Börner A. , 'Mapping of new 99 new microsatellite loci in rye (Secale cereale L.) including 39 expressed sequence tags ' (2004 ) 109 Theor. Appl. Genet. : 725 -732.

    • Search Google Scholar
  • Korzun, V., Röder, M.S., Wendehake, K., Pasqualone, A., Lotti, C., Ganal, M.W., Blanco, A. 1999. Integration of dinucleotide microsatellites from hexaploid bread wheat into a genetic linkage map of durum wheat. Theor. Appl. Genet. 98 :1202–1207.

    Blanco A. , 'Integration of dinucleotide microsatellites from hexaploid bread wheat into a genetic linkage map of durum wheat ' (1999 ) 98 Theor. Appl. Genet. : 1202 -1207.

    • Search Google Scholar
  • Laikova, L.I., Arbuzova, V.S., Efremova, T.T., Popova, O.M. 2004. Construction of immune lines with complex resistance to leaf rust and powdery mildew in common spring wheat cultivar Saratovskaya 29. Rus. J. Genet. 40 :506–509.

    Popova O.M. , 'Construction of immune lines with complex resistance to leaf rust and powdery mildew in common spring wheat cultivar Saratovskaya 29 ' (2004 ) 40 Rus. J. Genet. : 506 -509.

    • Search Google Scholar
  • Lander, E.S., Green, P., Abrahamson, J., Barlow, A., Daly, M.J., Linkoln, S.E., Newburg, L. 1987. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1 :174–181.

    Newburg L. , 'MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations ' (1987 ) 1 Genomics : 174 -181.

    • Search Google Scholar
  • Leonova, I.N., Röder, M.S., Budashkina, E.B., Kalinina, N.P., Salina, E.A. 2002. Molecular analysis of leaf rust resistant introgression lines obtained by crossing of hexaploid wheat Triticum aestivum with tetraploid wheat Triticum timopheevii . Rus. J. Genet. 38 :1397–1403.

    Salina E.A. , 'Molecular analysis of leaf rust resistant introgression lines obtained by crossing of hexaploid wheat Triticum aestivum with tetraploid wheat Triticum timopheevii ' (2002 ) 38 Rus. J. Genet. : 1397 -1403.

    • Search Google Scholar
  • Leonova, I., Börner, A., Budashkina, E., Kalinina, N., Unger, O., Röder, M., Salina, E. 2004. Identification of microsatellite markers for a leaf rust resistance gene introgressed into common wheat from Triticum timopheevii . Plant Breed. 123 :93–95.

    Salina E. , 'Identification of microsatellite markers for a leaf rust resistance gene introgressed into common wheat from Triticum timopheevii ' (2004 ) 123 Plant Breed. : 93 -95.

    • Search Google Scholar
  • Leonova, I.N., Laikova, L.I., Popova, O.M., Unger, O., Börner, A., Röder, M.S. 2007. Detection of quantitative trait loci for leaf rust resistance in wheat — T. timopheevii/T. tauschii introgression lines. Euphytica 155 :79–86.

    Röder M.S. , 'Detection of quantitative trait loci for leaf rust resistance in wheat — T. timopheevii/T. tauschii introgression lines ' (2007 ) 155 Euphytica : 79 -86.

    • Search Google Scholar
  • Mains, E.B., Jackson, H.S. 1926. Physiological specialization in the leaf rust of wheat, Puccinia triticina Erikss. Phytopathol. 16 :89–120.

    Jackson H.S. , 'Physiological specialization in the leaf rust of wheat, Puccinia triticina Erikss ' (1926 ) 16 Phytopathol. : 89 -120.

    • Search Google Scholar
  • McIntosh, R.A., 1983. Genetic and cytogenetic studies involving Lr18 for resistance to Puccinia recondita . In: S. Sakamota (ed.), Proc. 6 th Int. Wheat Genetics Symp., Kyoto, Japan, pp. 777–783.

  • McIntosh, R.A., Yamazaki, Y., Dubcovsky, J., Rogers, J., Morris, C., Somers, D.J., Apples, R., Devos, K.M. 2008. Catalogue of Gene Symbols for Wheat. http://www.shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp

  • Murphy, J.P., Navarro, R.A., Marshall, D., Cowger, C., Cox, T.S., Kolmer, J.A., Leath, S., Gaines, C.S. 2007. Registration of NC06BGTAG12 and NC06BGTAG13 powdery mildew-resistant wheat germplasm. J. Plant Regist. 1 :75–77.

    Gaines C.S. , 'Registration of NC06BGTAG12 and NC06BGTAG13 powdery mildew-resistant wheat germplasm ' (2007 ) 1 J. Plant Regist. : 75 -77.

    • Search Google Scholar
  • Pestsova, E., Ganal, M.W., Röder, M.S. 2000. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43 :689–697.

    Röder M.S. , 'Isolation and mapping of microsatellite markers specific for the D genome of bread wheat ' (2000 ) 43 Genome : 689 -697.

    • Search Google Scholar
  • Plaschke, J., Ganal, M.W., Röder, M.S. 1995. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 91 :1001–1007.

    Röder M.S. , 'Detection of genetic diversity in closely related bread wheat using microsatellite markers ' (1995 ) 91 Theor. Appl. Genet. : 1001 -1007.

    • Search Google Scholar
  • Prasad, M., Varshney, R.K., Roy, J.K., Balyan, H.S., Gupta, P.K. 2000. The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor. Appl. Genet. 100 :584–592.

    Gupta P.K. , 'The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat ' (2000 ) 100 Theor. Appl. Genet. : 584 -592.

    • Search Google Scholar
  • Röder, M.S., Plaschke, J., König, S.U., Börner, A., Sorrels, M.E., Tanksley, S.D., Ganal, M.W. 1995. Abundance, variability and chromosomal location of microsatellites in wheat. Mol. Gen. Genet. 246 :327–333.

    Ganal M.W. , 'Abundance, variability and chromosomal location of microsatellites in wheat ' (1995 ) 246 Mol. Gen. Genet. : 327 -333.

    • Search Google Scholar
  • Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149 :2007–2023.

    Ganal M.W. , 'A microsatellite map of wheat ' (1998 ) 149 Genetics : 2007 -2023.

  • Roelfs, A.P., Singh, R.P., Saari, E.E. 1992. Rust diseases of wheat: concepts and methods of disease management. CYMMYT, Mexico, D. F.

    Saari E.E. , '', in Rust diseases of wheat: concepts and methods of disease management , (1992 ) -.

  • Salina, E.A., Leonova, I.N., Efremova, T.T., Röder, M.S. 2006. Wheat genome structure: translocations during the course of polyploidization. Funct. Integr. Genomics 6 :71–80.

    Röder M.S. , 'Wheat genome structure: translocations during the course of polyploidization ' (2006 ) 6 Funct. Integr. Genomics : 71 -80.

    • Search Google Scholar
  • Sayre, K.D., Singh, R.P., Huerto-Espino, J., Rajaram, S. 1998. Genetic progress in reducing losses to leaf rust in CIMMIT-derived Mexican spring wheat cultivars. Crop Sci. 38 :654–659.

    Rajaram S. , 'Genetic progress in reducing losses to leaf rust in CIMMIT-derived Mexican spring wheat cultivars ' (1998 ) 38 Crop Sci. : 654 -659.

    • Search Google Scholar