Cereal Research Communications
Volume/Issue:
Volume 38: Issue 3
Heterologous expression and dough mixing studies of a novel cysteine-rich avenin-like protein
Authors:
P. ChenGuangxi University College of Agriculture Daxue Road 100 Nanning 530004 China

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R. LiGuangxi University College of Life Science and Technology Daxue Road 100 Nanning 530004 China

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R. ZhouGuangxi University College of Agriculture Daxue Road 100 Nanning 530004 China

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G. HeHuazhong University of Science & Technology China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory Luoyu Road 1037 Wuhan 430074 China

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P. ShewryRothamsted Research Harpenden, Hertfordshire AL5 2JQ UK

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Pages:
406–418
Online Publication Date:
27 Aug 2010
Publication Date:
01 Sep 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.38.2010.3.11
Keywords:
Avenin-like; wheat; Mixograph test; dough properties
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The Avenin-like gene (EU096532) was cloned from Aegilops biuncialis (2n = 4X, UUMM) in our previously study, the encoded gluten protein contained 19 cysteine residues, much more than that in all other glutenin subunits characterized so far. In present study, the protein was expressed in E. coli in large scale and purified in high purity through His-binding affinity chromatography. The purified protein was simply added or incorporated into a base flour and conducted with a 2 g Mixograph in order to investigate the functional properties including mixing time (MT), peak dough resistance (PR) and breakdown in resistance (RBD). Both 10 mg and 15 mg Avenin-like protein could cause significant increases in MT and PR, and decrease in RBD, compared to the control, when incorporated into dough. But the latter showed larger effect on functional properties. Size exclusion high-performance liquid chromatography (SE-HPLC) analysis confirmed that Avenin-like protein was chemically incorporated into polymeric subunits by intermolecular disulphide bonds.

  • Anderson, O.D., Hsia, C.C., Adalsteins, A.E., Lew, E.J.L., Kasarda, D.D. 2001. Identification of several new classes of low-molecular-weight wheat gliadin-related proteins and genes. Theor. Appl. Genet. 103 :307–315.

    Kasarda D.D. , 'Identification of several new classes of low-molecular-weight wheat gliadin-related proteins and genes ' (2001 ) 103 Theor. Appl. Genet. : 307 -315 .

    • Search Google Scholar

  • Barro, F., Barceló, P., Lazzeri, P.A., Shewry, P.R., Ballesteros, J., Martín, A. 2003. Functional properties of flours from field grown transgenic wheat lines expressing the HMW glutenin subunit 1Ax1 and 1Dx5 genes. Mol. Breeding 12 :223–229.

    Martín A. , 'Functional properties of flours from field grown transgenic wheat lines expressing the HMW glutenin subunit 1Ax1 and 1Dx5 genes ' (2003 ) 12 Mol. Breeding : 223 -229 .

    • Search Google Scholar

  • Barro, F., Barcelo, P., Rooke, L., Tatham, A.S., Bekes, F., Shewry, P.R., Lazzeri, P. 1997. Improvement of the processing properties of wheat by transformation with HMW subunits of glutenin. Nature Biotech. 15 :1295–1299.

    Lazzeri P. , 'Improvement of the processing properties of wheat by transformation with HMW subunits of glutenin ' (1997 ) 15 Nature Biotech. : 1295 -1299 .

    • Search Google Scholar

  • Batey, I.L., Gupta, R.B., MacRitchie, F. 1991. Use of size-exclusion high-performance liquid chromatography in the study of wheat flour proteins: an improved chromatographic procedure. Cereal Chem. 68 :207–209.

    MacRitchie F. , 'Use of size-exclusion high-performance liquid chromatography in the study of wheat flour proteins: an improved chromatographic procedure ' (1991 ) 68 Cereal Chem. : 207 -209 .

    • Search Google Scholar

  • Bean, S.R., Lookhart, G.L. 2001. Factors influencing the characterization of gluten proteins by size-exclusion chromatography and multiangle laser light scattering (SEC-MALLS). Cereal Chem. 78 :608–618.

    Lookhart G.L. , 'Factors influencing the characterization of gluten proteins by size-exclusion chromatography and multiangle laser light scattering (SEC-MALLS) ' (2001 ) 78 Cereal Chem. : 608 -618 .

    • Search Google Scholar

  • Bekes, F., Anderson, O.D., Gras, P.W., Gupta, R.B., Tam, A., Wrigley, C.W., Appels, R. 1994. The contribution to mixing properties of 1D HMW glutenin subunits expressed in a bacterial system. In: Henry, J.R., Ronalds, J.A. (eds), Improvement of Cereal Quality by Genetic Engineering. Marcel Dekker, New York, USA, pp. 97–104.

    Appels R. , '', in Improvement of Cereal Quality by Genetic Engineering , (1994 ) -.

  • Bekes, F., Gras, P.W. 1992. Demonstration of the 2-gram mixograph as a research tool. Cereal Chem. 69 :229–230.

    Gras P.W. , 'Demonstration of the 2-gram mixograph as a research tool ' (1992 ) 69 Cereal Chem. : 229 -230 .

    • Search Google Scholar

  • Blechl, A., Lin, J., Nguyen, S., Chan, R., Anderson, O.D., Dupont, F.M. 2007. Transgenic wheats with elevated levels of Dx5 and/or Dy10 high-molecular-weight glutenin subunits yield doughs with increased mixing strength and tolerance. J. Cereal Sci. 45 :172–183.

    Dupont F.M. , 'Transgenic wheats with elevated levels of Dx5 and/or Dy10 high-molecular-weight glutenin subunits yield doughs with increased mixing strength and tolerance ' (2007 ) 45 J. Cereal Sci. : 172 -183 .

    • Search Google Scholar

  • Clarke, B., Phongkham, T., Gianibelli, M., Beasley, H., Bekes, F. 2003. The characterisation and mapping of a family of LMW-gliadin genes: Effects on dough properties and bread volume. Theor. Appl. Genet. 106 :629–635.

    Bekes F. , 'The characterisation and mapping of a family of LMW-gliadin genes: Effects on dough properties and bread volume ' (2003 ) 106 Theor. Appl. Genet. : 629 -635 .

    • Search Google Scholar

  • Chen, P., Wang, C., Li, K., Chang, J., Wang, Y., Yang, G., Shewry, P.R., He, G. 2008. Cloning, expression and characterization of novel avenin-like genes in wheat and related species. J. Cereal Sci. 48 :734–740

    He G. , 'Cloning, expression and characterization of novel avenin-like genes in wheat and related species ' (2008 ) 48 J. Cereal Sci. : 734 -740 .

    • Search Google Scholar

  • Don, C., Lichtendonk, W.J., Plijter, J.J., Hamer, R.J. 2003. Understanding the link between GMP and dough:from glutenin particles in flour towards developed dough. J. Cereal Sci. 38 :157–165.

    Hamer R.J. , 'Understanding the link between GMP and dough:from glutenin particles in flour towards developed dough ' (2003 ) 38 J. Cereal Sci. : 157 -165 .

    • Search Google Scholar

  • Dupont, F.M., Chan, R., Lopez, R., Vensel, W.H. 2005. Sequential extraction and quantitative recovery of gliadins, glutenins and other proteins from small samples of wheat flour. J. Agr. Food Chem. 53 :1575–1584.

    Vensel W.H. , 'Sequential extraction and quantitative recovery of gliadins, glutenins and other proteins from small samples of wheat flour ' (2005 ) 53 J. Agr. Food Chem. : 1575 -1584 .

    • Search Google Scholar

  • Ferrante, P., Cristina Gianibelli, M., Larroque, O., Volpi, C., D’Ovidio, R., Lafiandra, D., Masci, S. 2006. Effect of incorporation of an i-type low-molecular-weight glutenin subunit and a modified g-gliadin in durum and in bread wheat doughs as measured by micro-mixographic analyses. J. Cereal Sci. 44 :194–202.

    Masci S. , 'Effect of incorporation of an i-type low-molecular-weight glutenin subunit and a modified g-gliadin in durum and in bread wheat doughs as measured by micro-mixographic analyses ' (2006 ) 44 J. Cereal Sci. : 194 -202 .

    • Search Google Scholar

  • Fido, R.J., Békés, F., Gras, P.W., Tatham, A.S. 1997. Effects of α-, β-, γ- and ω -gliadins on the dough mixing properties of wheat flour. J. Cereal Sci. 26 :271–277.

    Tatham A.S. , 'Effects of α-, β-, γ- and ω-gliadins on the dough mixing properties of wheat flour ' (1997 ) 26 J. Cereal Sci. : 271 -277 .

    • Search Google Scholar

  • Fredy, A., Vimla, V., Vibha, S., Indra, K.V. 1996. Integration and expression of the high-molecular-weight glutenin subunit 1Ax1 gene into wheat. Nature Biotech. 14 :1155–1159.

    Indra K.V. , 'Integration and expression of the high-molecular-weight glutenin subunit 1Ax1 gene into wheat ' (1996 ) 14 Nature Biotech. : 1155 -1159 .

    • Search Google Scholar

  • Gras, P.W., Anderssen, R.S., Keentok, M., Békés, F., Appels, R. 2001. Gluten protein functionality in wheat flour processing: A review. J. Agric. Res. 52 :1311–1323.

    Appels R. , 'Gluten protein functionality in wheat flour processing: A review ' (2001 ) 52 J. Agric. Res. : 1311 -1323 .

    • Search Google Scholar

  • Gras, P.W., Bekes, F. 1996. Small-scale testing: The development of instrumentation and application as a research tool. In: Wrigley, C.W. (ed.), Proc. 6th Int Gluten Workshop. Royal Australian Chemical Institute, Sydney, pp. 506–510.

    Bekes F. , '', in Proc. 6th Int Gluten Workshop , (1996 ) -.

  • He, G.Y., Jones, H.D., D’Ovidio, R., Masci, S., Chen, M.J., West, J., Butow, B., Anderson, O.D., Lazzeri, P., Fido, R., Shewry, P.R. 2005. Expression of an extended HMW subunit in transgenic wheat and the effect on dough mixing properties. J. Cereal Sci. 42 :225–231.

    Shewry P.R. , 'Expression of an extended HMW subunit in transgenic wheat and the effect on dough mixing properties ' (2005 ) 42 J. Cereal Sci. : 225 -231 .

    • Search Google Scholar

  • Johansson, E., Nilsson, H., Mazhar, H., Skerritt, J., MacRitchie, F., Svensson, G. 2002. Seasonal effects on storage proteins and gluten strength in four Swedish wheat cultivars. J. Sci. Food and Agriculture 82 :1305–1311.

    Svensson G. , 'Seasonal effects on storage proteins and gluten strength in four Swedish wheat cultivars ' (2002 ) 82 J. Sci. Food and Agriculture : 1305 -1311 .

    • Search Google Scholar

  • Kan, Y., Wan, Y., Beaudoin, F., Leader, D.J., Edwards, K., Poole, R., Wang, D., Mitchell, R.A.C., Shewry, P.R. 2006. Transcriptome analysis reveals differentially expressed storage protein transcripts in seeds of Aegilops and wheat. J. Cereal Sci. 44 :75–85.

    Shewry P.R. , 'Transcriptome analysis reveals differentially expressed storage protein transcripts in seeds of Aegilops and wheat ' (2006 ) 44 J. Cereal Sci. : 75 -85 .

    • Search Google Scholar

  • Kasarda, D.D. 1989. Glutenin structure in relation to wheat quality. In: Pomeranz, Y. (ed.), Wheat is Unique, American Association of Cereal Chemistry. St Paul, pp. 277–302.

  • Kasarda, D.D. 1999. Glutenin polymers: The in vitro to in vivo transition. Cereal Foods World 44 :566–571.

    Kasarda D.D. , 'Glutenin polymers: The in vitro to in vivo transition ' (1999 ) 44 Cereal Foods World : 566 -571 .

    • Search Google Scholar

  • Kuktaite, R., Larsson, H., Johansson, E. 2004. Variation in protein composition of wheat flour and its relationship to dough mixing behaviour. J. Cereal Sci. 40 :31–39.

    Johansson E. , 'Variation in protein composition of wheat flour and its relationship to dough mixing behaviour ' (2004 ) 40 J. Cereal Sci. : 31 -39 .

    • Search Google Scholar

  • Lee, Y.K., Bekes, F., Gras, P., Ciaffi, M., Morell, M.K., Appels, R. 1999. The low-molecular-weight glutenin subunit proteins of primitive wheats. IV. Functional properties of products from individual genes. Theor. Appl. Genet. 98 :149–155.

    Appels R. , 'The low-molecular-weight glutenin subunit proteins of primitive wheats ' (1999 ) 98 IV. Functional properties of products from individual genes. Theor. Appl. Genet. : 149 -155 .

    • Search Google Scholar

  • Li, W., Wan, Y., Liu, Z., Liu, K., Liu, X., Li, B., Li, Z., Zhang, X., Dong, Y., Wang, D. 2004. Molecular characterization of HMW glutenin subunit allele 1Bx14: further insights into the evolution of Glu-B1-1 alleles in wheat and related species. Theor. Appl. Genet. 109 :1093–1104.

    Wang D. , 'Molecular characterization of HMW glutenin subunit allele 1Bx14: further insights into the evolution of Glu-B1-1 alleles in wheat and related species ' (2004 ) 109 Theor. Appl. Genet. : 1093 -1104 .

    • Search Google Scholar

  • MacRitchie, F., Gupta, R.B. 1993. Functionality-composition relationships of wheat flour as a result of variation in sulfur availability. J. Agric. Res. 44 :1767–1774.

    Gupta R.B. , 'Functionality-composition relationships of wheat flour as a result of variation in sulfur availability ' (1993 ) 44 J. Agric. Res. : 1767 -1774 .

    • Search Google Scholar

  • Maforimbo, E., Skurray, G., Uthayakumaran, S., Wrigley, C. 2008. Incorporation of soy proteins into the wheat-gluten matrix during dough mixing. J. Cereal Sci. 47 :380–385.

    Wrigley C. , 'Incorporation of soy proteins into the wheat-gluten matrix during dough mixing ' (2008 ) 47 J. Cereal Sci. : 380 -385 .

    • Search Google Scholar

  • Nieto-Taladriz, M.T., Perretant, M.R., Rousset, M. 1994. Effect of gliadins and HMW and LMW subunits of glutenin on dough properties in the F6 recombinant inbred lines from a bread wheat cross. Theor. Appl. Genet. 88 :81–88.

    Rousset M. , 'Effect of gliadins and HMW and LMW subunits of glutenin on dough properties in the F6 recombinant inbred lines from a bread wheat cross ' (1994 ) 88 Theor. Appl. Genet. : 81 -88 .

    • Search Google Scholar

  • Payne, P.I. 1987. Genetics of wheat storage protein and the effect of allelic variation on breadmaking quality. An. Review Plant Physiol. 38 :141–153.

    Payne P.I. , 'Genetics of wheat storage protein and the effect of allelic variation on breadmaking quality ' (1987 ) 38 An. Review Plant Physiol. : 141 -153 .

    • Search Google Scholar

  • Primo-Martin, C., Valera, R., Martinez-Anaya, M.A. 2003. Effect of pentosanase and oxidases on the characteristics of doughs and the glutenin macropolymer (GMP). J. Agric. and Food Chem. 51 :4673–4679.

    Martinez-Anaya M.A. , 'Effect of pentosanase and oxidases on the characteristics of doughs and the glutenin macropolymer (GMP) ' (2003 ) 51 J. Agric. and Food Chem. : 4673 -4679 .

    • Search Google Scholar

  • Rakszegi, M., Békés, F., Láng, L., Tamás, L., Shewry, P.R., Bedõ, Z. 2005. Technological quality of transgenic wheat expressing an increased amount of a HMW glutenin subunit. J. Cereal Sci. 42 :15–23.

    Bedõ Z. , 'Technological quality of transgenic wheat expressing an increased amount of a HMW glutenin subunit ' (2005 ) 42 J. Cereal Sci. : 15 -23 .

    • Search Google Scholar

  • Rath, C.R., Gras, P.W., Wrigley, C.W., Walker, C.E. 1990. Evaluation of dough properties from two grams of flour using the mixograph principle. Cereal Foods World 35 :572–574.

    Walker C.E. , 'Evaluation of dough properties from two grams of flour using the mixograph principle ' (1990 ) 35 Cereal Foods World : 572 -574 .

    • Search Google Scholar

  • Rooke, L., Bekes, F., Fido, R., Barro, F., Gras, P., Tatham, A.S., Barcelo, P., Lazzeri, P., Shewry, P.R. 1999. Overexpression of a gluten protein in transgenic wheat results in greatly increased dough strength. J. Cereal Sci. 30 :115–120.

    Shewry P.R. , 'Overexpression of a gluten protein in transgenic wheat results in greatly increased dough strength ' (1999 ) 30 J. Cereal Sci. : 115 -120 .

    • Search Google Scholar

  • Salcedo, G., Prada, J., Aragoncillo, C. 1979. LowMWgliadin-like proteins from wheat endosperm. Phytochem. 18 :725–727.

    Aragoncillo C. , 'LowMWgliadin-like proteins from wheat endosperm ' (1979 ) 18 Phytochem. : 725 -727 .

    • Search Google Scholar

  • Schober, T.J., Bean, S.R., Kuhn, M. 2006. Gluten proteins from spelt ( Triticum aestivum ssp. spelta ) cultivars: A rheological and size-exclusion high-performance liquid chromatography study. J. Cereal Sci. 44 :161–173.

    Kuhn M. , 'Gluten proteins from spelt (Triticum aestivum ssp. spelta) cultivars: A rheological and size-exclusion high-performance liquid chromatography study ' (2006 ) 44 J. Cereal Sci. : 161 -173 .

    • Search Google Scholar

  • Shewry, P.R. 1995. Plant storage proteins. Biol. Rev. Camb. Philos. Soc. 70 :375–426.

    Shewry P.R. , 'Plant storage proteins ' (1995 ) 70 Biol. Rev. Camb. Philos. Soc. : 375 -426 .

    • Search Google Scholar

  • Shewry, P.R., Halford, N.G. 2002. Cereal seed storage proteins: Structures, properties and role in grain utilization. J. Exp. Botany 53 :947–958.

    Halford N.G. , 'Cereal seed storage proteins: Structures, properties and role in grain utilization ' (2002 ) 53 J. Exp. Botany : 947 -958 .

    • Search Google Scholar

  • Shewry, P.R., Tatham, A.S., Lazzeri, P. 1997. Biotechnology of wheat quality. J. Sci. Food and Agric. 73 :397–406.

    Lazzeri P. , 'Biotechnology of wheat quality ' (1997 ) 73 J. Sci. Food and Agric. : 397 -406 .

    • Search Google Scholar

  • Skerritt, J.H., Hac, L., Bekes, F. 1999. Depolymerization of the glutenin macropolymer during dough mixing: I. Changes in levels, molecular weight distribution, and overall composition. Cereal Chem. 76 :395–401.

    Bekes F. , 'Depolymerization of the glutenin macropolymer during dough mixing: I. Changes in levels, molecular weight distribution, and overall composition ' (1999 ) 76 Cereal Chem. : 395 -401 .

    • Search Google Scholar

  • Suchy, J., Lukow, O.M., Ingelin, M.E. 2000. Dough microextensibility method using a 2-g mixograph and a texture analyzer. Cereal Chem. 77 :39–43.

    Ingelin M.E. , 'Dough microextensibility method using a 2-g mixograph and a texture analyzer ' (2000 ) 77 Cereal Chem. : 39 -43 .

    • Search Google Scholar

  • Tamtam, L., Shewry, P.R. 2006. Heterologous expression and protein engineering of wheat gluten proteins. J. Cereal Sci. 43 :259–274.

    Shewry P.R. , 'Heterologous expression and protein engineering of wheat gluten proteins ' (2006 ) 43 J. Cereal Sci. : 259 -274 .

    • Search Google Scholar

  • Veraverbeke, W.S., Verbruggen, I.M., Delcour, J.A. 2001. Effects of gliadin fractions on functional properties of wheat dough depending on molecular size and hydrophobicity. Cereal Chem. 78 :138–141.

    Delcour J.A. , 'Effects of gliadin fractions on functional properties of wheat dough depending on molecular size and hydrophobicity ' (2001 ) 78 Cereal Chem. : 138 -141 .

    • Search Google Scholar

  • Verbruggen, I.M., Veraverbeke, W.S., Delcour, J.A. 2001. Significance of LMW-GS and HMW-GS for dough extensibility: “Addition” versus “incorporation” protocols. J. Cereal Sci. 33 :253–260.

    Delcour J.A. , 'Significance of LMW-GS and HMW-GS for dough extensibility: “Addition” versus “incorporation” protocols ' (2001 ) 33 J. Cereal Sci. : 253 -260 .

    • Search Google Scholar

  • Weegels, P.L., Hamer, R.J., Schofield, J.D. 1997. Depolymerisation and re-polymerisation of wheat glutenin during dough processing. II. Changes in composition. J. Cereal Sci. 25 :155–163.

    Schofield J.D. , 'Depolymerisation and re-polymerisation of wheat glutenin during dough processing. II. Changes in composition ' (1997 ) 25 J. Cereal Sci. : 155 -163 .

    • Search Google Scholar

  • Wieser, H. 2007. Chemistry of gluten proteins. Food Microbiol. 24 :115–119.

    Wieser H. , 'Chemistry of gluten proteins ' (2007 ) 24 Food Microbiol. : 115 -119 .

  • Wieser, H., Bushuk, W., MacRitchie, F. 2006. The polymeric glutenins. In: Wrigley, C., Bekes, F., Bushuk, W. (eds.), Gliadin and Glutenin: The Unique Balance of Wheat Quality. St. Paul, Am. Ass. Cereal Chem., pp. 213–240.

    MacRitchie F. , '', in Gliadin and Glutenin: The Unique Balance of Wheat Quality , (2006 ) -.

  • Wieser, H., Kieffer, R. 2001. Correlations of the amount of gluten protein types to the technological properties of wheat flours determined on a micro-scale. J. Cereal Sci. 34 :19–27.

    Kieffer R. , 'Correlations of the amount of gluten protein types to the technological properties of wheat flours determined on a micro-scale ' (2001 ) 34 J. Cereal Sci. : 19 -27 .

    • Search Google Scholar

  • William, H.V., Charlene, K.T., Nick, C., Joshua, H.W., Buchanan, B.B., Hurkman, W.J. 2005. Developmental changes in the metabolic protein profiles of wheat endosperm. Proteomics 5 :1594–1611.

    Hurkman W.J. , 'Developmental changes in the metabolic protein profiles of wheat endosperm ' (2005 ) 5 Proteomics : 1594 -1611 .

    • Search Google Scholar

  • Xu, H., Wang, R., Shen, X., Zhao, Y., Sun, G., Zhao, H., Guo, A. 2006. Functional properties of a new low-molecular-weight glutenin subunit gene from a bread wheat cultivar. Theor. Appl. Genet. 113 :1295–1303.

    Guo A. , 'Functional properties of a new low-molecular-weight glutenin subunit gene from a bread wheat cultivar ' (2006 ) 113 Theor. Appl. Genet. : 1295 -1303 .

    • Search Google Scholar

  • Zhu, Y.F., Li, Y.W., Chen, Y., Li, H., Liang, H., Yue, S.J., Zhang, A.M., Zhang, X.Q., Wang, D.W., Jia, X. 2005. Generation and characterization of a high molecular weight glutenin 1Bx14-deficient mutant in common wheat. Plant Breeding 124 :421–427.

    Jia X. , 'Generation and characterization of a high molecular weight glutenin 1Bx14-deficient mutant in common wheat ' (2005 ) 124 Plant Breeding : 421 -427 .

    • Search Google Scholar
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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
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Cereal Research Communications
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ISSN 0133-3720 (Print)
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