Cereal Research Communications
Volume/Issue:
Volume 38: Issue 4
New approaches for the study of osmotic stress induced by polyethylene glycol (PEG) in cereal species
Authors:
A. Comeau Agriculture and Agri-Food Canada Quebec City QC G1V 2J3 Canada

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L. Nodichao Centre de Recherches Agricoles Plantes Pérennes BP01 Pobè Bénin

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J. Collin Laval University Dept. Phytology Quebec City QC G1V 0A6 Canada

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M. Baum ICARDA PO Box 5466 Aleppo Syria

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J. Samsatly ICARDA PO Box 5466 Aleppo Syria

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D. Hamidou Nouakchott University Biology Department Nouakchott Mauritania

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F. Langevin Agriculture and Agri-Food Canada Quebec City QC G1V 2J3 Canada

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A. Laroche Agriculture and Agri-food Canada Lethbridge AB T1J 4B1 Canada

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E. Picard Université Paris Sud XI F-91405 Orsay Cedex 1 France

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Pages:
471–481
Online Publication Date:
16 Dec 2010
Publication Date:
01 Dec 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.38.2010.4.3
Keywords:
drought; osmotic stress; maize; wheat; barley; rye; triticale; oats; Pennisetum
Restricted access

Literature confirms that using polyethylene glycol (PEG) as an osmotic agent to imitate water shortage was not so easy in practice, due to PEG toxicity effects and frequent contaminations. Two new approaches were developed to alleviate those problems, one using a raft covered with a membrane to prevent PEG entry in roots, and one using solidified PEG media. The raft trials were done on corn, hexaploid and tetraploid wheat, rye, triticale, oats, barley, Agrotricum; those in solid media, with corn, hexaploid and tetraploid wheat, barley, sorghum and pearl millet. Different species respond differently to PEG-induced osmotic stress. In our trials, the most sensitive cereal was corn, and this finding correlates with the lower osmotic pressure of the sap (a constitutive trait in corn seedlings). Corn responded to osmotic stress by a very poor rate of elongation of the coleoptile, especially when the highest stress (32% PEG) was used. This behavior was also observed in the field in dry years, for example in the Sahel area. Compared to this sensitive cereal species, all other cereals tested were more resistant. Hexaploid and tetraploid wheat, triticale, and Agrotricum kept capacity to elongate roots when submitted to a high osmotic stress, but the higher stress reduced root length considerably. Barley kept rooting ability like other cereals, but was able to develop more aerial biomass, seminal roots, and ramifications. Barley root hair was also longer and covered a higher proportion of the root. Those adaptive features likely explain part of the good adaptation of barley to dry Mediterranean areas. Preliminary results on solid media also showed relationships between drought resistance and the osmoresistance response, at least when comparing species. Roots of species adapted to hot climate, like pearl millet and sorghum, had few seminal roots but displayed a strong gravitropism under osmotic stress. The ease of use of solidified PEG media shows promise for future larger scale trials. Applications of solidified PEG media for research beyond cereal crops is envisioned.

  • Attree, S.M. 2003. Increasing levels of growth regulator and/or water stress during embryo development. US patent 6627441.

  • Barnabás, B., Jäger, K., Fehér, A. 2008. The effect of drought and heat on reproductive processes in cereals. Plant Cell Environ. 31:11–38.

    Fehér A. , 'The effect of drought and heat on reproductive processes in cereals ' (2008 ) 31 Plant Cell Environ : 11 -38 .

    • Search Google Scholar

  • Baum, M., Grando, S., Backes, G., Jahoor, A., Sabbagh, A., Ceccarelli, S. 2003. QTLs for agronomic traits in the Mediterranean environment identified in recombinant inbred lines of the cross ‘Arta’ × H. spontaneum 41-1. Theor. Appl. Genet. 107:1215–1225.

    Ceccarelli S. , 'QTLs for agronomic traits in the Mediterranean environment identified in recombinant inbred lines of the cross ‘Arta’ × H. spontaneum 41-1 ' (2003 ) 107 Theor. Appl. Genet. : 1215 -1225 .

    • Search Google Scholar

  • Blum, A. 2000. Use of PEG to induce and control plant water deficit in experimental hydroponics culture. http://www.plantstress.com/methods/PEG.htm

  • Blum, A. 2005. Drought resistance, water use efficiency, and yield potential — are they compatible, dissonant, or mutually exclusive? Aust. J. Agric. Res. 56:1159–1168.

    Blum A. , 'Drought resistance, water use efficiency, and yield potential — are they compatible, dissonant, or mutually exclusive? ' (2005 ) 56 Aust. J. Agric. Res. : 1159 -1168 .

    • Search Google Scholar

  • Castonguay, Y., Markhart III, A.H. 1992. Leaf exchange in water-stressed common bean and tepary bean. Crop Sci. 32:980–986.

    Markhart A.H. , 'Leaf exchange in water-stressed common bean and tepary bean ' (1992 ) 32 Crop Sci. : 980 -986 .

    • Search Google Scholar

  • Ceccarelli, S., Grando, S., Baum, M., Udupa, S.M. 2004. Breeding for drought resistance in a changing climate. In: Challenges and strategies for dryland agriculture. CSSSA Special Publ. no. 32. pp. 167–190.

  • Grando, S., Ceccarelli, S. 2009. Breeding for resistance to abiotic stresses. In: Ceccarelli, S., Guiamares, E.P., Weltzien, E. (eds), Plant Breeding and Farmer Participation. FAO, Rome, Italy. pp. 391–417.

    Ceccarelli S. , '', in Plant Breeding and Farmer Participation , (2009 ) -.

  • Hao, X., de Jong, E. 1988. Growth of wheat and barley seedlings at different matric and osmotic potentials. Agron. J. 80:807–811.

    Jong E. , 'Growth of wheat and barley seedlings at different matric and osmotic potentials ' (1988 ) 80 Agron. J. : 807 -811 .

    • Search Google Scholar

  • Hasse, H., Kany, H.P., Tintinger, R., Maurer, G. 1995. Osmotic virial coefficients of aqueous poly(ethyleneglycol) from laser-light scattering and isopiestic measurements. Macromolecules 28:3540–3552.

    Maurer G. , 'Osmotic virial coefficients of aqueous poly(ethyleneglycol) from laser-light scattering and isopiestic measurements ' (1995 ) 28 Macromolecules : 3540 -3552 .

    • Search Google Scholar

  • Huang, B. 2000. Role of root morphological characteristics in drought resistance of plants. In: Wilkinson, RE. (ed.), Plant-Environment Interactions. Marcel Dekker, NY., USA. pp. 39–64.

    Huang B. , '', in Plant-Environment Interactions , (2000 ) -.

  • McClendon, J.H. 1981. The osmotic pressure of concentrated solutions of polyethylene glycol 6000, and its variation with temperature. J. Exp. Bot. 32:861–866.

    McClendon J.H. , 'The osmotic pressure of concentrated solutions of polyethylene glycol 6000, and its variation with temperature ' (1981 ) 32 J. Exp. Bot. : 861 -866 .

    • Search Google Scholar

  • Michel, B.E. 1983. Evaluation of the water potential of solutions of polyethylene glycol 8000 both in the absence and presence of other solutes. Plant Physiol. 72:66–70.

    Michel B.E. , 'Evaluation of the water potential of solutions of polyethylene glycol 8000 both in the absence and presence of other solutes ' (1983 ) 72 Plant Physiol : 66 -70 .

    • Search Google Scholar

  • Nodichao, L. 1997. Biodiversité racinaire et résistance à la sécheresse [Root system biodiversity and drought resistance]. M.S. thesis. Laval U. 168 pp. (in French)

  • Plaut, Z., Federman, E. 1985. A simple procedure to overcome polyethylene glycol toxicity on whole plants. Plant Physiol. 79:559–561.

    Federman E. , 'A simple procedure to overcome polyethylene glycol toxicity on whole plants ' (1985 ) 79 Plant Physiol. : 559 -561 .

    • Search Google Scholar

  • Rajaram, S., van Ginkel, M. 2001. Mexico: 50 years of International Wheat Breeding (chapter 22). In: Bonjean, A.P., Angus, W.J. (eds), The World Wheat Book. A history of wheat breeding. Lavoisier Publishing, Paris, France. pp. 579–608

    Ginkel M. , '', in The World Wheat Book. A history of wheat breeding , (2001 ) -.

  • Sharp, R.E., Silk, W.K., Hsiao, T.C. 1988. Growth of the maize primary root at low water potentials. I. Spatial distribution of expansive growth. Plant Physiol. 87:50–57.

    Hsiao T.C. , 'Growth of the maize primary root at low water potentials. I. Spatial distribution of expansive growth ' (1988 ) 87 Plant Physiol. : 50 -57 .

    • Search Google Scholar

  • Steuter, A.A., Mozafar, A., Goodin, J.R. 1981. Water potential of aqueous polyethylene glycol. Plant Physiol. 67:64–67.

    Goodin J.R. , 'Water potential of aqueous polyethylene glycol ' (1981 ) 67 Plant Physiol : 64 -67 .

    • Search Google Scholar

  • Szira, F., Bálint, A.F., Börner, A., Galiba, G. 2008. Evaluation of drought-related traits and screening methods at different developmental stages in barley. J. Agron. Crop Sci. 194:334–342.

    Galiba G. , 'Evaluation of drought-related traits and screening methods at different developmental stages in barley ' (2008 ) 194 J. Agron. Crop Sci. : 334 -342 .

    • Search Google Scholar

  • Tabuchi, T., Kawaguchi, Y., Azuma, T., Nanmori, T., Yasuda, T. 2005. Similar regulation patterns of choline monooxygenase, phosphoethanolamine n-methyltransferase and s-adenosyl-l-methionine synthetase in leaves of the halophyte Atriplex nummularia L. Plant Cell Physiol. 46:505–513.

    Yasuda T. , 'Similar regulation patterns of choline monooxygenase, phosphoethanolamine n-methyltransferase and s-adenosyl-l-methionine synthetase in leaves of the halophyte Atriplex nummularia L ' (2005 ) 46 Plant Cell Physiol : 505 -513 .

    • Search Google Scholar

  • Teulat, B., Rekika, D., Nachit, M., Monneveux, P. 1997. Comparative adjustment in barley and tetraploid wheats. Plant Breeding 116:519–523.

    Monneveux P. , 'Comparative adjustment in barley and tetraploid wheats ' (1997 ) 116 Plant Breeding : 519 -523 .

    • Search Google Scholar

  • Tingey, D.T., Stockwell, C. 1977. Semipermeable membrane system for subjecting plants to water-stress. Plant Physiol. 60:58–62.

    Stockwell C. , 'Semipermeable membrane system for subjecting plants to water-stress ' (1977 ) 60 Plant Physiol : 58 -62 .

    • Search Google Scholar

  • Walter, H. 1985. Vegetation of the Earth. 3rd ed. Heidelberg Sc. Lib. p. 138.

  • Winzor, D.J. 2004. Reappraisal of disparities between osmolality estimates by freezing point depression and vapor pressure deficit methods. Biophys. Chem. 107:317–323.

    Winzor D.J. , 'Reappraisal of disparities between osmolality estimates by freezing point depression and vapor pressure deficit methods ' (2004 ) 107 Biophys. Chem. : 317 -323 .

    • Search Google Scholar

  • Yan, W., Tinker, N.A. 2005. An integrated biplot analysis system for displaying, interpreting, and exploring genotype-environment interaction. Crop Sci. 45:1004–1016.

    Tinker N.A. , 'An integrated biplot analysis system for displaying, interpreting, and exploring genotype-environment interaction ' (2005 ) 45 Crop Sci. : 1004 -1016 .

    • Search Google Scholar
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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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