Authors: J. Li, G. He 2 and W. Cram 2
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  • 1 Taizhou University School of Life Science Linhai, Zhejiang 317000 China
  • 2 Huazhong University of Science & Technology (HUST) China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, the Genetic Engineering International Cooperation base of Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Luoyu Road 1037 Wuhan, Hubei 430074 China
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To identify feedback signal(s) controlling nitrate uptake by wheat roots, the effects of 13 amino acids on time course of net nitrate influx have been investigated. A new non-invasive technique of measuring net nitrate influx with a high time resolution of minutes has enabled us to make this comparison. The results showed that glutamate caused a rapid and large decrease of net nitrate influx by roots. Aspartic acid, asparagine, glutamine caused a relatively slow decrease of net nitrate influx with a lag of 0.5–1.5 h. Other amino acids had a small effect or even no effect. It is speculated that glutamate is most likely to be a negative feedback signal.

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