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T. Sumíková

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J. Remešová

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L. LeišováCrop Research Institute Prague 6 161 06 Czech Republic

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L. KuceraCrop Research Institute Prague 6 161 06 Czech Republic

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J. ChrpováCrop Research Institute Prague 6 161 06 Czech Republic

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V. ŠípCrop Research Institute Prague 6 161 06 Czech Republic

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Amplified fragment length polymorphism (AFLP) analysis was applied to genotype 186 Czech isolates of the Fusarium species responsible for head blight disease in wheat. Using 15 primer combinations, 694 polymorphic fragments were detected. The effective number of patterns was dependent on both the identity of the selective primer combination and on which Fusarium species was involved. The most informative primer combinations were CAT + AT, CAT + AC and CTA + AG, and a triplex assay based on CAT was able to distinguish all 186 isolates from each other. A principal component analysis identified six phylogenetic clusters, and this genetic architecture was supported by an analysis of population structure. AFLP profiling is an appropriate method for uncovering differences between these groups of Fusarium species.

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Cereal Research Communications
Language English
Size A4
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)