Cereal Research Communications
Volume/Issue:
Volume 39: Issue 2
Development of TaqMan assays for 3ADON, 15ADON and NIV Fusarium genotypes based on Tri12 gene
Author:
T. KulikUniversity of Warmia and Mazury in Olsztyn Department of Diagnostics and Plant Pathophysiology Plac Łódzki 5 Olsztyn 10-957 Poland

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Pages:
200–214
Online Publication Date:
21 May 2011
Publication Date:
01 Jun 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.39.2011.2.4
Keywords:
Fusarium culmorum ; F. graminearum ; 3ADON; 15ADON and NIV producers; TaqMan assays
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Fungi of the genus Fusarium are important pathogens of numerous crops including cereals. Infection of the grain with Fusarium spp. often results in the accumulation of various mycotoxins which poses a significant risk to food safety. The Fusarium graminearum species complex, together with F. culmorum, are the main source of trichothecenes in cereal grains. They are comprised of fungal strains producing one of three specific trichothecenes: either 3ADON (3-acetyldeoxynivalenol), 15ADON (15-acetyldeoxynivalenol) (not present in F. culmorum) or NIV (nivalenol). Recently, qualitative PCR approaches have been developed for rapid determination of 3ADON, 15ADON and NIV genotypes within F. graminearum/F. culmorum. This paper presents the development of three TaqMan genotyping assays for quantitative diagnostics of 3ADON, 15ADON and NIV genotypes within F. graminearum/F. culmorum. Primers and probes were designed on the basis of 95 nucleotide site within the Tri12 gene which allowed for specific determination of trichothecene producers. Additionally, to address the problem of detecting inhibitors, an internal positive control (IPC) was developed employing primers and a probe derived from conserved fungal sequence of 5.8S rDNA. The assays developed were successfully applied for direct determination of trichothecene genotypes from Fusarium-damaged wheat kernels (FDK).

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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