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  • 1 Institute of Field and Vegetable Crops Maksima Gorkog 30 Novi Sad Serbia
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A Serbian government introduced a labeling system for products which contained genetically modified organisms (GMO) with threshold of 0.1% for grain and 0.9% for food and feed.In order to detect the presence of GM maize in the Serbian market, a triplex PCR was applied to maize grains and processed maize samples. In total 145 samples, 125 samples of maize seed and 25 processed maize were analysed in tree years period. The number of positive samples varied from 12% in 2006 year, to 14.5% in 2008. The estimated amounts of GM maize using Real Time PCR in 17 samples ranged between 0.1% and 0.9%, for most of them 0.2% or 0.3%. In two maize seed samples GMO content was higer than >0.9%, in 2006. Obtained results showed that maize seed has been contaminated with GM seed but in most cases under labeling treshold.Monitoring for transgenic crop plants is necessary, especially in cases, like in Serbia, when the crop has not been approved for release into the environment.

  • Agbios, 2007. Biotech Crops Database. Available at http://www.agbios.com/dbase.php. AGBIOS, Merrickville, Ontario, Canada

    Agbios , '', in Biotech Crops Database , (2007 ) -.

  • Bush, U., Mühlbauer, B., Böhm, U., Liebl, B. 2001. Genetically changed maize and soy — qualitative and quantitative proof in food. German Food Review 97:125–129.

    Liebl B. , 'Genetically changed maize and soy — qualitative and quantitative proof in food ' (2001 ) 97 German Food Review : 125 -129.

    • Search Google Scholar
  • Farid, E.A. 2002. Detection of genetically modified organisms in food. Trends Biotechnol. 20:215–223.

    Farid E.A. , 'Detection of genetically modified organisms in food ' (2002 ) 20 Trends Biotechnol. : 215 -223.

    • Search Google Scholar
  • Greiner, R., Konietzny, U., Villavicencio, A.L.C.H. 2005. Qualitative and quantitative detection of genetically modified maize and soy in processed foods sold commercially in Brazil by PCR-based methods. Food Control 16:753–759.

    Villavicencio A.L.C.H. , 'Qualitative and quantitative detection of genetically modified maize and soy in processed foods sold commercially in Brazil by PCR-based methods ' (2005 ) 16 Food Control : 753 -759.

    • Search Google Scholar
  • Holst-Jensen, A., Ronning, S.B., Lovseth, A., Berdal, K. 2003. PCR technology for screening and quantification of genetically modified organisms (GMOs). Analytical and Bioanalytical Chem. 375:958–993.

    Berdal K. , 'PCR technology for screening and quantification of genetically modified organisms (GMOs) ' (2003 ) 375 Analytical and Bioanalytical Chem. : 958 -993.

    • Search Google Scholar
  • James, C. 2009. Global Status of Commercialized Biotech/GM Crops: 2009. ISAAA Brief 41. ISAAA, Ithaca, NY, USA.

    James C. , '', in Global Status of Commercialized Biotech/GM Crops: 2009 , (2009 ) -.

  • Lin, Y., Chiang, L.C., Shih, Y.C. 2001. Detection of genetically modified soy beans by PCR method and immunoassay kit. J. Food and Drug Anal. 8:200–207.

    Shih Y.C. , 'Detection of genetically modified soy beans by PCR method and immunoassay kit ' (2001 ) 8 J. Food and Drug Anal. : 200 -207.

    • Search Google Scholar
  • Lipp, M., Brodmann, P., Pietsch, K., Pauwels, J., Anklam, E. 1999. IUPAc collaborative trial study of a method to detect genetically modified soybeans and maize in dried powder. J. AOAC Int. 82:923–928.

    Anklam E. , 'IUPAc collaborative trial study of a method to detect genetically modified soybeans and maize in dried powder ' (1999 ) 82 J. AOAC Int. : 923 -928.

    • Search Google Scholar
  • Lipp, M., Bluth, A., Eyquem, F., Kruse, L., Schimmel, H., Van den Eede, G., Anklam, E. 2001. Validation of a method based on polymerase chain reaction for the detection of genetically modified organism in various procesed foodstuffs. Eur. Food Res. Technol. 212:497–504.

    Anklam E. , 'Validation of a method based on polymerase chain reaction for the detection of genetically modified organism in various procesed foodstuffs ' (2001 ) 212 Eur. Food Res. Technol. : 497 -504.

    • Search Google Scholar
  • Lipp, M., Shillito, R., Giroux, R., Spiegelhalter, F., Charlton, S., Pinero, D., Song, P. 2005. Polymerase chain reaction technology as analytical tool in agricultural biotechnology. J. of AOAC Internat. 88:136–155.

    Song P. , 'Polymerase chain reaction technology as analytical tool in agricultural biotechnology ' (2005 ) 88 J. of AOAC Internat. : 136 -155.

    • Search Google Scholar
  • Mazzara, M., Querci, M. 2002. The analysis of food samples for the presence of genetically modified organisms. European Commission Joint Research Centre. Session 7, pp. 6–18.

  • Miraglia, M., Berdal, K.G., Corbisier, P., Holst-Jensen, A. 2004. Detection and traceability of genetically modified organisms in the food production chain. Food Chem. Toxicol. 42:1157–1180.

    Holst-Jensen A. , 'Detection and traceability of genetically modified organisms in the food production chain ' (2004 ) 42 Food Chem. Toxicol. : 1157 -1180.

    • Search Google Scholar
  • Nikolih, Z., Miloševih, M., Vujaković, M., Marinković, D., Jevtić, A., Balešević-Tubić, S. 2008. Qualitative triplex PCR for the detection of genetically modified soybean and maize. Biotechnology and Biotechnological Equipment 3:801–803.

    Balešević-Tubić S. , 'Qualitative triplex PCR for the detection of genetically modified soybean and maize ' (2008 ) 3 Biotechnology and Biotechnological Equipment : 801 -803.

    • Search Google Scholar
  • Official Record of the Federal Republic of Yugoslavia 2001. Law on Genetically Modified Organisms. The Public Enterprise Official Gazzete, Belgrade, Serbia, No. 21/2001.

  • Official Gazette of the Repuplic Serbia and Montenegro 2003. Rulebook on “Labeling of genetically modified products”. The Public Enterprise Official Gazzete, Belgrade, Serbia, No. 6/2003.

  • Official Gazette of the Republic of Serbia 2009. Law on Genetically Modified Organisms. The Public Enterprise Official Gazzete, Belgrade, Serbia, No. 41/2009.

  • Pauli, U., Liniger, M., Zimmerman, A., Schrott, M. 2000. Extraction and amplification of DNA from 55 foodstuffs. Mitteilungen aus Lebensmitteluntersuchung und Hygiene 91:491–501.

    Schrott M. , 'Extraction and amplification of DNA from 55 foodstuffs ' (2000 ) 91 Mitteilungen aus Lebensmitteluntersuchung und Hygiene : 491 -501.

    • Search Google Scholar
  • Querci, M., Van den Eede, G., Jermini, M. 2004. Overview, general introduction on genetically modified organisms (GMOs), EU legislation. In: Querci, M., Jermini, M., Van den Eede, G. (eds), The Analysis of Food Samples for the Presence of Genetically Modified Organisms. Special Publication No. I.03.114, European Commision, Joint Research Centre, Italy, Session 1, pp. 3–12.

    Jermini M. , '', in The Analysis of Food Samples for the Presence of Genetically Modified Organisms , (2004 ) -.

    • Search Google Scholar
  • Somma, M. 2004. Extraction and purification of DNA. In: Querci, M., Jermini, M., Van den Eede, G. (eds), The Analysis of Food Samples for the Presence of Genetically Modified Organisms. Special Publication No. I.03.114, European Commision, Joint Research Centre, Italy, Session 4, pp. 13–17.

    Somma M. , '', in The Analysis of Food Samples for the Presence of Genetically Modified Organisms , (2004 ) -.

    • Search Google Scholar
  • Studer, E., Dahinden, I., Lüthy, J., Hübner, P. 1997. Detection of the genetically engineered Maximizer Maize using the polymerase chain reaction (PCR). Mitt. Gebiete Lebensm. Hyg. 88: 515–524.

    Hübner P. , 'Detection of the genetically engineered Maximizer Maize using the polymerase chain reaction (PCR) ' (1997 ) 88 Mitt. Gebiete Lebensm. Hyg. : 515 -524.

    • Search Google Scholar
  • Waiblinger, H.U., Graf, N., Mäde, D., Woll, K., Busch, U., Holland, B., Pilsl, H., Naeumann, G., Reiting, R., Ehrentreich, B., Schulze, M., Tschirdewahn, B., Brünen-Nieweler, C., Hempel, G., Weidner, M., Winterstein, A.R. 2007. “Technically unavoidable” in terms of genetically modified organisms — an approach for food control. J. Verbr. Lebensm. 2:126–129.

    Winterstein A.R. , '“Technically unavoidable” in terms of genetically modified organisms — an approach for food control ' (2007 ) 2 J. Verbr. Lebensm. : 126 -129.

    • Search Google Scholar