Cereal Research Communications
Volume/Issue:
Volume 39: Issue 3
Sequence polymorphism and expression analysis of genes encoding gibberellin 20-oxidase from Dasypyrus villosum
Authors:
P. Cai

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H. Long Chinese Academy of Sciences Chengdu Institute of Biology Chengdu, Sichuan 610041 China

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J. Liang Chinese Academy of Sciences Chengdu Institute of Biology Chengdu, Sichuan 610041 China

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C. Wang Chinese Academy of Sciences Chengdu Institute of Biology Chengdu, Sichuan 610041 China

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G. Deng Chinese Academy of Sciences Chengdu Institute of Biology Chengdu, Sichuan 610041 China

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Z. Pan Chinese Academy of Sciences Chengdu Institute of Biology Chengdu, Sichuan 610041 China

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Z. Peng

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M. Yu Chinese Academy of Sciences Chengdu Institute of Biology Chengdu, Sichuan 610041 China

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Pages:
365–375
Online Publication Date:
12 May 2011
Publication Date:
01 Sep 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.2011.001
Keywords:
gibberellin 20-oxidase; Dasypyrum villosum ; wheat; gene cloning; SNP
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Based on the known GA20-oxidase (GA20ox) cDNAs of barley and wheat, oligonucleotide primers were designed to isolate GA20ox genes from genomic DNA of Dasypyrum villosum. A total of 19 clones were obtained. Each of them contained an open reading frame encoding a putative 40-KDa protein of 359 amino acid residues. Twenty-one SNPs and 4 InDels were found and could divide the 19 sequences into 2 classes, designated as DvGA20ox-1 and DvGA20ox-2, respectively. Q-PCR analyses showed that both DvGA20ox-1 and DvGA20ox-2 were in leaf blade, leaf sheath, stem, eustipes, root and developing spike. Similar expression levels were found between DvGA20ox-1 and DvGA20ox-2 in three stages. The total expression levels of DvGA20ox-1 and DvGA20ox-2 presented downtrend in leaf blade and ascend in stem, eustipes and developing spike along with the development of plants, respectively. However, they were firstly increased and then decreased in root from seeding stage to heading stage. These results revealed that the gene expression profile of DvGA20ox-1 and DvGA20ox-2 closely related to the growth and development of D. villosum.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
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Foundation		 1973
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per Year		 4
Founder Akadémiai Kiadó
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Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
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Springer Nature Switzerland AG
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ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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