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  • 1 Iranian Research Institute of Plant Protection Tehran P.O. Box 19395-1454 Iran
  • | 2 University of Guilan Department of Plant Protection, Faculty of Agriculture Rasht 41635-1314 P.O. Box 41889 Iran
  • | 3 University of Tehran Department of Plant Protection, Faculty of Agriculture Karaj 3158711167 Iran
  • | 4 University of Szeged Department of Microbiology Közép fasor 52 H-6726 Szeged Hungary
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As a first step of a project aimed at the identification of potential biocontrol agents of Rhizoctonia solani, the rice sheath blight fungus, we surveyed the biodiversity of the genus Trichoderma based on sequence of the internal transcribed spacer (ITS) 1 and 2 of the ribosomal RNA gene cluster in paddy fields in Mazandaran province, Northern Iran. Amongst the six obtained species of Trichoderma, T. harzianum and T. virens proved to be the most frequent species in this habitat. Sequence alignment and phylogenetic analysis revealed that the T. harzianum isolates can be divided into 14 different ITS genotypes clustering in four groups. Our results are in agreement with previous molecular studies, which also revealed that T. harzianum is a complex species comprising more or less different ITS genotypes. T. virens was not as diverse as T. harzianum and three different genotypes were distinguished which constituted only one cluster. All T. atroviride and T. hamatum strains had identical ITS sequences.

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