Phenotypic and epigenetic changes occurred during the autopolyploidization of Aegilops tauschii

Z. Zeng; T. Zhang; G. Li; C. Liu; Z. Yang

doi:10.1556/crc.40.2012.0014

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Cereal Research Communications

Volume/Issue:: Volume 40: Issue 4

Phenotypic and epigenetic changes occurred during the autopolyploidization of Aegilops tauschii

Authors:

Z. Zeng

Z. Zeng University of Electronic Science and Technology of China School of Life Science and Technology 4 Jianshe Road Chengdu 610054 China

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T. Zhang

T. Zhang University of Electronic Science and Technology of China School of Life Science and Technology 4 Jianshe Road Chengdu 610054 China

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G. Li

G. Li University of Electronic Science and Technology of China School of Life Science and Technology 4 Jianshe Road Chengdu 610054 China

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C. Liu

C. Liu University of Electronic Science and Technology of China School of Life Science and Technology 4 Jianshe Road Chengdu 610054 China

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Z. Yang

Z. Yang University of Electronic Science and Technology of China School of Life Science and Technology 4 Jianshe Road Chengdu 610054 China

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Pages:: 476–485

Online Publication Date:: 20 Nov 2014

Publication Date:: 01 Dec 2012

Article Category:: Research Article

DOI:: https://doi.org/10.1556/crc.40.2012.0014

Keywords:: autopolyploidization; DNA methylation; Aegilops tauschii

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A synthetic autopolyploid was developed from diploid Aegilops tauschii, D genome progenitor of common wheat (Triticum aestivum). The tetraploid Ae. tauschii displayed a markedly larger organ size than the diploid donor. Fluorescence in situ hybridization (FISH) and DNA marker analysis revealed that there is no clear variation at either the chromosomal or DNA level between the diploid and tetraploid plants. We analyzed the variation in cytosine methylation patterns between the diploid and tetraploid plants by methylation-sensitive amplified polymorphism (MSAP) and detected 228 and 232 methylated sites in diploid and tetraploid plants, respectively. Statistical comparison indicated that the tetraploid Ae. tauschii genotype displayed no significant difference in polymorphic methylation level compared to the diploid ones. Twenty-two different genomic fragments displaying different methylation behavior during the ploidy conversions were isolated and sequenced. It demonstrated that alterations in the level of methylation have the most profound effects on coding genes. We demonstrated that there are some genes expressions modified by DNA methylation may be correlated with phenotypic alteration after autotetraploidization.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)