Authors:
S. Wang Capital Normal University Key Laboratory of Genetics and Biotechnology, College of Life Science 100048 Beijing China

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D. Chen Capital Normal University Key Laboratory of Genetics and Biotechnology, College of Life Science 100048 Beijing China

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G. Guo Capital Normal University Key Laboratory of Genetics and Biotechnology, College of Life Science 100048 Beijing China

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T. Zhang Capital Normal University Key Laboratory of Genetics and Biotechnology, College of Life Science 100048 Beijing China

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S. Jiang Capital Normal University Key Laboratory of Genetics and Biotechnology, College of Life Science 100048 Beijing China

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X. Shen Capital Normal University Key Laboratory of Genetics and Biotechnology, College of Life Science 100048 Beijing China

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D. Perovic Julius Kuehn-Institute (JKI) Erwin-Baur-Str. 27 D-06484 Quedlinburg Germany

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S. Prodanovic University of Belgrade Faculty of Agriculture Nemanjina 6 11080 Belgrade Serbia

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Y. Yan Capital Normal University Key Laboratory of Genetics and Biotechnology, College of Life Science 100048 Beijing China

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In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.

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Cereal Research Communications
Language English
Size A4
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)