Crown rot caused by Fusarium pathogens is one of the most economically destructive diseases of wheat. The objective of this study was to investigate defense-related enzyme responses in wheat seedlings infected with Fusarium graminearum. Three wheat varieties were employed to the experiment. Physiological analysis of varieties indicated that defensive-related enzymes were induced by F. graminearum infection. The superoxide dismutase and peroxidase activities increased dramatically and the phenylalanine ammonia-lyase activity was stimulated to a higher level in resistant variety Hongyouzi than in susceptible variety Wo0102. However, the chitinase activity was higher in susceptible variety Wo0102 than in Hongyouzi after infection. These results suggest that the defense enzymes could be stimulated in wheat seedlings by Fusarium infection.
Akinsanmi, O.A., Mitter, V., Simpfendorfer, S., Backhouse, D., Chakraborty, S. 2004. Identity and pathogenicity of Fusarium spp. Isolated from wheat fields in Queensland and northern New South Wales. Aust. J. Agric. Res. 55:97–107.
Chakraborty S. , 'Identity and pathogenicity of Fusarium spp. Isolated from wheat fields in Queensland and northern New South Wales ' (2004 ) 55 Aust. J. Agric. Res. : 97 -107.
Bai, G.H., Plattner, R., Desjardins, A., Kolb, F.L. 2001. Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat. Plant Breeding 120:1–6.
Kolb F.L. , 'Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat ' (2001 ) 120 Plant Breeding : 1 -6.
Beauchamp, C., Fridovich, I. 1971. Superoxide dismutase: Improved assays and assay applicable to acrylamide gels. Anal. Biochem. 44:276–287.
Fridovich I. , 'Superoxide dismutase: Improved assays and assay applicable to acrylamide gels ' (1971 ) 44 Anal. Biochem. : 276 -287.
Benhamou, N. 1996. Elicitor-induced plant defense pathways. Trends Plant Sci. 1:233–240.
Benhamou N. , 'Elicitor-induced plant defense pathways ' (1996 ) 1 Trends Plant Sci. : 233 -240.
Bol, J.F., Linthorst, J.M., Cornelissen, B.J.C. 1990. Plant pathogenesis — related proteins induced by virus infection. Annu. Rev. Phytopathol. 28:113–138.
Cornelissen B.J.C. , 'Plant pathogenesis — related proteins induced by virus infection ' (1990 ) 28 Annu. Rev. Phytopathol. : 113 -138.
Boller, T., Metraux, J.P. 1988. Colorimentric assay for chitinase. Method Enzymol. 161:430–435.
Metraux J.P. , 'Colorimentric assay for chitinase ' (1988 ) 161 Method Enzymol. : 430 -435.
Brisson, L.F., Tenhaken, R., Lamb, C.J. 1994. Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance. The Plant Cell 6:1703–1712.
Lamb C.J. , 'Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance ' (1994 ) 6 The Plant Cell : 1703 -1712.
Desmond, O.J., Edgar, C.I., Manners, J.M. 2006. Methyl jasmonate induced gene expression in wheat delays symptom development by the crown rot pathogen Fusarium pseudograminearum. Physiol. Mol. Plant P 67:171–179.
Manners J.M. , 'Methyl jasmonate induced gene expression in wheat delays symptom development by the crown rot pathogen Fusarium pseudograminearum ' (2006 ) 67 Physiol. Mol. Plant P : 171 -179.
Hammerschmidt, R., Nuckles, E.M., Kuc, J. 1982. Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiol. Plant Path. 20:73–82.
Kuc J. , 'Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium ' (1982 ) 20 Physiol. Plant Path. : 73 -82.
Jetiyanon, K. 2007. Defensive-related enzyme response in plants treated with a mixture of Bacillus strains (IN937a and IN937b) against different pathogens. Biology Control 42:178–185.
Jetiyanon K. , 'Defensive-related enzyme response in plants treated with a mixture of Bacillus strains (IN937a and IN937b) against different pathogens ' (2007 ) 42 Biology Control : 178 -185.
Joosten, M.H.A.J., Witt, P.J.G.M. de. 1989. Identification of several pathogenesis-related proteins in tomato leaves inoculated with Cladosporium fulvum (syn. Fulvia fulva) as b-1,3-glucanases and chitinases. Plant Physiol. 89:945–951.
Witt P.J.G.M. , 'Identification of several pathogenesis-related proteins in tomato leaves inoculated with Cladosporium fulvum (syn. Fulvia fulva) as b-1,3-glucanases and chitinases ' (1989 ) 89 Plant Physiol. : 945 -951.
Marasas, W.F.O., Voigt, W.G.J., Lamprecht, S.C., Van Wyk, P.S. 1988. Crown rot and head blight of wheat caused by Fusarium graminearum groups 1 and 2 in the southern Cape province. Phytophylactica 20:385–389.
Wyk P.S. , 'Crown rot and head blight of wheat caused by Fusarium graminearum groups 1 and 2 in the southern Cape province ' (1988 ) 20 Phytophylactica : 385 -389.
Mitter, V., Zhang, M.C., Liu, C.J., Ghosh, R., Ghosh, M., Chakraborty, S. 2006. A high-throughput glasshouse bioassay to detect crown rot resistance in wheat germplasm. Plant Pathol. 55:433–441.
Chakraborty S. , 'A high-throughput glasshouse bioassay to detect crown rot resistance in wheat germplasm ' (2006 ) 55 Plant Pathol. : 433 -441.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7:405–410.
Mittler R. , 'Oxidative stress, antioxidants and stress tolerance ' (2002 ) 7 Trends Plant Sci. : 405 -410.
Roldán Serrano, A., Luna del Castillo, J., Jorrín Novo, J., Fernández Ocaña, A., Gómez Rodríguez, M.V. 2007. Chitinase and peroxidase activities in sunflower hypocotyls: Effects of BTH and inoculation with Plasmopara halstedii. Biology Plantarum 51:149–152.
Gómez Rodríguez M.V. , 'Chitinase and peroxidase activities in sunflower hypocotyls: Effects of BTH and inoculation with Plasmopara halstedii ' (2007 ) 51 Biology Plantarum : 149 -152.
Shi, Z.Q., Wang, F., Zhou, W., Zhang, P., Fan, Y.J. 2007. Application of osthol induces a resistance response against powdery mildew in pumpkin leaves. Int. J. Molecular Sci. 8:1001–1012.
Fan Y.J. , 'Application of osthol induces a resistance response against powdery mildew in pumpkin leaves ' (2007 ) 8 Int. J. Molecular Sci. : 1001 -1012.
Wildermuth, G.B., McNamara, R.B. 1994. Testing wheat seedlings for resistance to crown rot caused by Fusarium graminearum group 1. Plant Disease 78:949–953.
McNamara R.B. , 'Testing wheat seedlings for resistance to crown rot caused by Fusarium graminearum group 1 ' (1994 ) 78 Plant Disease : 949 -953.
Wildermuth, G.B., McNamara, R.B., Quick, J.S. 2001. Crown depth and susceptibility to crown rot in wheat. Euphytica 122:397–405.
Quick J.S. , 'Crown depth and susceptibility to crown rot in wheat ' (2001 ) 122 Euphytica : 397 -405.
Zhang, X., Zhou, M.P., Ren, L., Bai, G.H., Ma, H.X., Scholten, O.E., Guo, P.G., Lu, W.Z. 2004. Molecular characterization of Fusarium head blight resistance from wheat cultivar Wangshuibai. Euphytica 139:59–64.
Lu W.Z. , 'Molecular characterization of Fusarium head blight resistance from wheat cultivar Wangshuibai ' (2004 ) 139 Euphytica : 59 -64.