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  • 1 Mendel University in Brno Faculty of Agronomy Zemědělská 1 613 00 Brno Czech Republic
  • 2 Tomas Bata University in Zlín Department of Food Technology and Microbiology, Faculty of Technology nám. T. G. Masaryka 5555 760 01 Zlín Czech Republic
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The research was conducted to describe distribution of protein fractions in wheat (Triticum aestivum L.) mill products (semolina, flour and bran) and evaluate the possibility of prediction of protein fractions distribution from values of bread-making quality (protein and gluten content, Zeleny sedimentation volume) evaluated on wholemeal and specific flour. The content of protein fractions was determined by size-exclusion high performance liquid chromatography. Significantly highest glutenin content was found in flour (5.01%). The investigated mill products did not differ in gliadin content, the content of albumin/globulin fraction reached the highest values in bran (3.60%). The model of prediction of glutenin and gliadin content in mill products explained 31–62% and 83–92% of the original variability. The protein fractions distribution in wheat mill products could be satisfactorily predicted from known values of protein and gluten content evaluated on wholemeal and Zeleny sedimentation volume evaluated on specific flour.

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