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  • 1 Mendel University in Brno, CEITEC MENDELU Department of Plant Biology Brno 61300 Czech Republic
  • 2 Mendel University in Brno, CEITEC MENDELU Central European Institute of Technology Brno 61300 Czech Republic
  • 3 Agrotest Fyto, Ltd. Kroměříž 76701 Czech Republic
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Polymerase chain reaction (PCR) was used to identify the allelic composition of Glu-A1, Glu-B1 and Glu-D1 loci that comprise the high-molecular-weight (HMW) glutenin subunits in 15 triticale genotypes. The Glu-A1b allele was detected most frequently (86.7%) at the Glu-A1 locus; the Glu-A1a and Glu-A1c alleles were only found, respectively, in the ‘Pawo’ and ‘Leontino’ varieties. Five allelic combinations were detected at Glu-B1 [Glu-B1-1a+2o (encoding Bx7+By8*), Glu-B1-1a+2s (Bx7+By18*), Glu-B1-1a+2z (Bx7+By20*), Glu-B1-1b+2a (Bx7*+By8) and Glu-B1-1b+2o (Bx7*+By8*)]. The Glu-D1d allele (HMW subunits 5+10) was observed in breeding lines that carried translocated segments of wheat chromosome 1D. Dough mixing results indicated the Glu-D1d allele provided slight improvements in bread-making quality. Three-year results with Glu-B1b+2o/2a encoding subunits Bx7*+By8/ By8* exhibited positive affects in bread-making quality. These subunits were detected in varieties ‘Mungis’ and ‘Pawo’.

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