Cereal Research Communications
Volume/Issue:
Volume 41: Issue 4
Genetic variation of wheat landraces from China as revealed by gliadin and microsatellite markers
Authors:
X. Li

Search for other papers by X. Li in
Current site
Google Scholar
PubMed Close
,
X. Xu

Search for other papers by X. Xu in
Current site
Google Scholar
PubMed Close
,
X. Li Chinese Academy of Agricultural Sciences The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences Beijing 100081 China

Search for other papers by X. Li in
Current site
Google Scholar
PubMed Close
,
X. Yang Chinese Academy of Agricultural Sciences The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences Beijing 100081 China

Search for other papers by X. Yang in
Current site
Google Scholar
PubMed Close
,
W. Liu Chinese Academy of Agricultural Sciences The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences Beijing 100081 China

Search for other papers by W. Liu in
Current site
Google Scholar
PubMed Close
, and
L. Li Chinese Academy of Agricultural Sciences The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences Beijing 100081 China

Search for other papers by L. Li in
Current site
Google Scholar
PubMed Close
Pages:
573–582
Online Publication Date:
14 Oct 2013
Publication Date:
01 Dec 2013
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.41.2013.4.7
Keywords:
genetic diversity; gliadin; microsatellite; wheat landrace
Restricted access

A reborn interest has occurred during the last decade toward wheat landraces for broadening genetic basis of modern wheat cultivars. The investigation of molecular traits within and between existing landraces of wheat can help scientists to develop appropriate strategies for their efficient maintenance and exploitation. The present study dealt with the gliadin characterization of forty-seven wheat landraces collected from wheat mainly planted areas of China, each of which was represented by a sample of at least 43 individuals. Twelve accessions selected on the basis of gliadin analysis were investigated further using 21 SSR markers. The results proved that landraces of wheat are a mixture of variable individuals genetically distinguishable from each other. Twelve of the analyzed 47 accessions were observed to be homogeneous, while 35 (74.5%) of them were heterogeneous in their gliadin composition. In total, 122 gliadin pattern were observed. On average, 10.1% (Gst) of the total variation arose from differentiation among regions, and 89.9% was attributed to within-region variation. Furthermore, nineteen of the 21 SSR markers were polymorphic across all the populations. The total number of the amplified DNA products was 110, with a mean of 6.11 alleles per locus. The values of genetic diversity within each landrace population varied from 0.006 to 0.351. Implications for the management of this valuable genetic resource are discussed.

  • Amirouche, R., Misset, M.T. 2003. Hordein polymorphism in diploid and tetraploid mediterranean populations of the Hordeum murinum L. complex. Plant Syst. Evol. 242:83–99.

    Misset M.T. , 'Hordein polymorphism in diploid and tetraploid mediterranean populations of the Hordeum murinum L. complex ' (2003 ) 242 Plant Syst. Evol. : 83 -99 .

    • Search Google Scholar

  • Caballero, L., Martýn, L.M., Alvarez, J.B. 2004. Variation and genetic diversity for gliadins in Spanish spelt wheat accessions. Genet. Resour. Crop Evol. 51:679–686.

    Alvarez J.B. , 'Variation and genetic diversity for gliadins in Spanish spelt wheat accessions ' (2004 ) 51 Genet. Resour. Crop Evol. : 679 -686 .

    • Search Google Scholar

  • Chwedorzewska, K.J., Bednarek, P.T., Puchalski, J. 2002. Studies on changes in specific rye genome regions due to seed aging and regeneration. Cell Mol. Biol. Lett. 7:569–576.

    Puchalski J. , 'Studies on changes in specific rye genome regions due to seed aging and regeneration ' (2002 ) 7 Cell Mol. Biol. Lett. : 569 -576 .

    • Search Google Scholar

  • Gregová, E., Hermuth, J., Kraic, J., Dotlačil, L. 1999. Protein heterogeneity in European wheat landraces and obsolete cultivars. Genet. Resour. Crop Evol. 46:521–528.

    Dotlačil L. , 'Protein heterogeneity in European wheat landraces and obsolete cultivars ' (1999 ) 46 Genet. Resour. Crop Evol. : 521 -528 .

    • Search Google Scholar

  • He, D.X., Li, H.J., Xu, S.C., Duan, X.Y., Zhou, Y.L., Li, L.H. 2007. Reaction to powdery mildew and stripe rust in related species and landraces of wheat. Genet. Resour. Crop Evol. 54:213–219.

    Li L.H. , 'Reaction to powdery mildew and stripe rust in related species and landraces of wheat ' (2007 ) 54 Genet. Resour. Crop Evol. : 213 -219 .

    • Search Google Scholar

  • Hoisington, D., Khairallah, M., Reeves, T., Ribaut, J.M., Skovmand, B., Taba, S., Warburton, M. 1999. Plant genetic resources: What can they contribute toward increased crop productivity? Proc. Natl. Acad. Sci. USA 96:5937–5943.

    Warburton M. , 'Plant genetic resources: What can they contribute toward increased crop productivity? ' (1999 ) 96 Proc. Natl. Acad. Sci. USA : 5937 -5943 .

    • Search Google Scholar

  • Huang, X.Q., Börner, A., Röder, M.S., Ganal, M.W. 2002. Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Theor. Appl. Genet. 105:699–707.

    Ganal M.W. , 'Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers ' (2002 ) 105 Theor. Appl. Genet. : 699 -707 .

    • Search Google Scholar

  • Li, X.J., Xu, X., Yang, X.M., Li, X.Q., Liu, W.H., Gao, A.N., Li, L.H. 2012. Genetic diversity of the wheat landrace Youzimai from different geographic regions investigated with morphological traits, seedling resistance to powdery mildew, gliadin and microsatellite markers. Cereal Res. Commun. 40:95–106.

    Li L.H. , 'Genetic diversity of the wheat landrace Youzimai from different geographic regions investigated with morphological traits, seedling resistance to powdery mildew, gliadin and microsatellite markers ' (2012 ) 40 Cereal Res. Commun. : 95 -106 .

    • Search Google Scholar

  • Liu, Y., Xiong, Z.Y., He, Y.G., Shewry, P.R., He, G.Y. 2007. Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province. Genet. Resour. Crop Evol. 54:865–874.

    He G.Y. , 'Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province ' (2007 ) 54 Genet. Resour. Crop Evol. : 865 -874 .

    • Search Google Scholar

  • Metakovsky, E.V., Branlard, G. 1998. Genetic diversity of French common wheat germplasm based on gliadin alleles. Theor. Appl. Genet. 96:209–218.

    Branlard G. , 'Genetic diversity of French common wheat germplasm based on gliadin alleles ' (1998 ) 96 Theor. Appl. Genet. : 209 -218 .

    • Search Google Scholar

  • Metakovsky, E.V., Novoselskaya, A.Yu. 1991. Gliadin allele identification in common wheat. I. Methodological aspects of the analysis of gliadin patterns by one-dimensional polyacrylamide gel electrophoresis. J. Genet. Breed. 5:317–324.

    Novoselskaya A.Yu. , 'Gliadin allele identification in common wheat. I. Methodological aspects of the analysis of gliadin patterns by one-dimensional polyacrylamide gel electrophoresis ' (1991 ) 5 J. Genet. Breed. : 317 -324 .

    • Search Google Scholar

  • Nevo, E., Pagnotta, M.A., Beiles, A., Poreeddu, E. 1995. Wheat storage proteins: glutenin DNA diversity in wild emmer wheat, Triticum dicoccoides, in Israel and Turkey. 3. Environmental correlates and allozymic associations. Theor. Appl. Genet. 91:415–420.

    Poreeddu E. , 'Wheat storage proteins: glutenin DNA diversity in wild emmer wheat, Triticum dicoccoides, in Israel and Turkey. 3. Environmental correlates and allozymic associations ' (1995 ) 91 Theor. Appl. Genet. : 415 -420 .

    • Search Google Scholar

  • Obert, D.E., Fritz, A.K., Moran, J.L., Singh, S., Rudd, J.C., Menz, M.A. 2005. Identification and molecular tagging of a gene from PI 289824 conferring resistance to leaf rust (Puccinia triticina) in wheat. Theor. Appl. Genet. 110:1439–1444.

    Menz M.A. , 'Identification and molecular tagging of a gene from PI 289824 conferring resistance to leaf rust (Puccinia triticina) in wheat ' (2005 ) 110 Theor. Appl. Genet. : 1439 -1444 .

    • Search Google Scholar

  • Pagnotta, M.A., Impiglia, A., Tanzarella, O.A., Nachit, M.M., Porceddu, E. 2004. Genetic variation of the durum wheat landrace Haurani from different agro-ecological regions. Genet. Resour. Crop Evol. 51:863–869.

    Porceddu E. , 'Genetic variation of the durum wheat landrace Haurani from different agro-ecological regions ' (2004 ) 51 Genet. Resour. Crop Evol. : 863 -869 .

    • Search Google Scholar

  • Pestsova, E., Röder, M. 2002. Microsatellite analysis of wheat chromosome 2D allows the reconstruction of chromosomal inheritance in pedigrees of breeding programmes. Theor. Appl. Genet. 106:84–91.

    Röder M. , 'Microsatellite analysis of wheat chromosome 2D allows the reconstruction of chromosomal inheritance in pedigrees of breeding programmes ' (2002 ) 106 Theor. Appl. Genet. : 84 -91 .

    • Search Google Scholar

  • Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S., Rafalski, A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breeding 2:225–238.

    Rafalski A. , 'The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis ' (1996 ) 2 Mol. Breeding : 225 -238 .

    • Search Google Scholar

  • Routray, P., Basha, O., Garg, M., Singh, N.K., Dhaliwal, H.S. 2007. Genetic diversity of landraces of wheat (Triticum aestivum L.) from hilly areas of Uttaranchal, India. Genet. Resour. Crop Evol. 54:1315–1326.

    Dhaliwal H.S. , 'Genetic diversity of landraces of wheat (Triticum aestivum L.) from hilly areas of Uttaranchal, India ' (2007 ) 54 Genet. Resour. Crop Evol. : 1315 -1326 .

    • Search Google Scholar

  • Saghai Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA 81:8014–8018.

    Allard R.W. , 'Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics ' (1984 ) 81 Proc. Natl. Acad. Sci. USA : 8014 -8018 .

    • Search Google Scholar

  • Wu, J., Yang, X., Wang, H., Li, H., Li, L., Li, X., Liu, W. 2006. The introgression of chromosome 6P specifying for increased numbers of florets and kernels from Agropyron cristatum into wheat. Theor. Appl. Genet. 114:13–20.

    Liu W. , 'The introgression of chromosome 6P specifying for increased numbers of florets and kernels from Agropyron cristatum into wheat ' (2006 ) 114 Theor. Appl. Genet. : 13 -20 .

    • Search Google Scholar

  • Zhang, L., Li, H., Wang, H., Li, L. 2007. Genetic diversification of the Chinese wheat landrace Mazhamai as revealed by morphological characteristics, seed storage proteins, and microsatellite markers. Can. J. Plant Sci. 87:763–777.

    Li L. , 'Genetic diversification of the Chinese wheat landrace Mazhamai as revealed by morphological characteristics, seed storage proteins, and microsatellite markers ' (2007 ) 87 Can. J. Plant Sci. : 763 -777 .

    • Search Google Scholar

  • Zillman, R.R., Bushuk, W. 1979a. Wheat cultivar identification by gliadin electrophoregrams. II. Effects of environmental and experimental factors on the gliadin electrophoregrams. Can. J. Plant Sci. 59:281–286.

    Bushuk W. , 'Wheat cultivar identification by gliadin electrophoregrams. II. Effects of environmental and experimental factors on the gliadin electrophoregrams ' (1979 ) 59 Can. J. Plant Sci. : 281 -286 .

    • Search Google Scholar

  • Zillman, R.R., Bushuk, W. 1979b. Wheat cultivar identification by gliadin electrophoregrams. III. Catalogue of electrophoregram formulas of Canadian wheat cultivars. Can. J. Plant Sci. 59:287–298.

    Bushuk W. , 'Wheat cultivar identification by gliadin electrophoregrams. III. Catalogue of electrophoregram formulas of Canadian wheat cultivars ' (1979 ) 59 Can. J. Plant Sci. : 287 -298 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

 

 

For subscription options, please visit the website of Springer Nature.

Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Dec 2024 200 0 0
Jan 2025 117 0 0
Feb 2025 111 0 0
Mar 2025 152 0 0
Apr 2025 26 0 0
May 2025 2 0 0
Jun 2025 0 0 0