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  • 1 Plant Breeding and Acclimatization Institute - National Research Institute, Radzików Department of Plant Pathology 05-870 Bŀonie Poland
  • | 2 Plant Breeding and Acclimatization Institute - National Research Institute, Radzików Department of Plant Breeding and Genetics 05-870 Bŀonie Poland
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A partially automated method of DNA isolation (a Freedom Evo workstation coupled with a paramagnetic bead-based DNA isolation kit from Machery-Nagel) was evaluated in terms of DNA quantity, purity, and quality in comparison with a conventional manual method (the membrane-based Qiagen Plant DNeasy kit). In addition, the efficiency in terms of time and cost was evaluated. The partially automated method enabled the isolation from each sample of DNA of good A260/A280 ratio and sufficient quantity to serve as a template for dozens of PCR amplifications suitable for conventional marker-assisted selection applications. However, lower PCR efficiencies, presumably due to lower DNAquality, were obtained with DNA extracted by the partially automated method as compared with the Qiagen method. The partially automated method presented here was shown to be superior to the conventional Qiagen method in that the costs and time required were reduced by ca. 50%. The Freedom Evo workstation coupled with Machery-Nagel DNA isolation kit appeared advantageous for high-throughput applications required for plant molecular breeding.

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Cereal Research Communications
Language English
Size B5
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
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Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
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Address
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ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)