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  • 1 University College Dublin School of Biology and Environmental Science Belfield, Dublin 4 Ireland
  • | 2 Umeå University Umeå Plant Science Centre, Department of Plant Physiology SE-901 87 Umeå Sweden
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Brachypodium distachyon is emerging as the model species for temperate monocotyledonous grasses of the Pooideae, and the genome of the B. distachyon community inbred line Bd21 has recently been sequenced. Here, we report the development of a procedure for the efficient establishment of a cell suspension culture derived from calli. We show that embryogenic potential is maintained in 3-month-old cultures as the cells were positively labelled by the monoclonal antibody, JIM8 which recognizes a carbohydrate epitope often present in arabinogalactan proteins found in the cell walls of cells in embryogenic cultures. Additionally, we were able to regenerate plants from these cell suspension cultures. The cell suspension culture we have established can also be used in studies on plant programmed cell death (PCD). Our results clearly demonstrate that B. distachyon cells can undergo apoptosis-like PCD (AL-PCD) as visualised by the characteristic retraction of the protoplast from the cell wall. As B. distachyon is genetically related to important temperate cereal grass crops like wheat and barley, the ability to utilise cell suspension cultures of B. distachyon to dissect the underlying mechanisms PCD will have important implications for understanding developmental processes in economically important cereal crops.

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Editor(s)-in-Chief: Pauk, János

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Cereal Research Communications
Language English
Size B5
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
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Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)