Characterization of intergeneric hybrid between common wheat and Aegilops sharonensis (Eig) and transfer of alien high molecular weight glutenin subunits into wheat

Q.Z. Zhao; Q.T. Jiang; Q. Yang; J. Ma; J.R. Wang; G.Y. Chen; Y.X. Liu; Z.X. Lu; Y.M. Wei; Y.L. Zheng

doi:10.1556/crc.2014.0001

Cereal Research Communications

Volume/Issue:: Volume 42: Issue 4

Characterization of intergeneric hybrid between common wheat and Aegilops sharonensis (Eig) and transfer of alien high molecular weight glutenin subunits into wheat

Authors:

Q.Z. Zhao

Q.Z. Zhao Sichuan Agricultural University Triticeae Research Institute Chengdu, Sichuan 611130 China

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Q.T. Jiang

Q.T. Jiang Sichuan Agricultural University Triticeae Research Institute Chengdu, Sichuan 611130 China

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Q. Yang

Q. Yang Sichuan Agricultural University College of Agronomy Chengdu, Sichuan 611130 China

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J. Ma

J. Ma Sichuan Agricultural University Triticeae Research Institute Chengdu, Sichuan 611130 China

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J.R. Wang

J.R. Wang Sichuan Agricultural University Triticeae Research Institute Chengdu, Sichuan 611130 China

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G.Y. Chen

G.Y. Chen Sichuan Agricultural University Triticeae Research Institute Chengdu, Sichuan 611130 China

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Y.X. Liu

Y.X. Liu Sichuan Agricultural University Triticeae Research Institute Chengdu, Sichuan 611130 China

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Z.X. Lu

Z.X. Lu Agriculture and Agri-Food Canada Lethbridge Research Centre Lethbridge T1J 4B1 Canada

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Y.M. Wei

Y.M. Wei Sichuan Agricultural University Triticeae Research Institute Chengdu, Sichuan 611130 China

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Y.L. Zheng

Y.L. Zheng

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Pages:: 640–647

Online Publication Date:: 15 Jun 2014

Publication Date:: 01 Dec 2014

Article Category:: Research Article

DOI:: https://doi.org/10.1556/crc.2014.0001

Keywords:: Aegilops sharonensis ; common wheat; cytology; HMW-GS; SSR marker

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Aegilops sharonensis (Sharon goatgrass) is a valuable source of novel high molecular weight glutenin subunits, resistance to wheat rust, powdery mildew, and insect pests. In this study, we successfully hybridized Ae. sharonensis as the pollen parent to common wheat and obtained backcross derivatives. F1 intergeneric hybrids were verified using morphological observation and cytological and molecular analyses. The phenotypes of the hybrid plants were intermediate between Ae. sharonensis and common wheat. Observations of mitosis in root tip cells and meiosis in pollen mother cells revealed that the F1 hybrids possessed 28 chromosomes. Chromosome pairing at metaphase I of the pollen mother cells in the F1 hybrid plants was low, and the meiotic configuration was 25.94 I + 1.03 II (rod). Two pairs of primers were screened out from 150 simple sequence repeat markers, and primer WMC634 was used to identified the presence of the genome of Ae. sharonensis. Sequencing results showed that the F1 hybrids contained the S^sh genome of Ae. sharonensis. The sodium dodecyl sulfate polyacrylamide gel electrophoresis profile showed that the alien high molecular weight glutenin subunits of Ae. sharonensis were transferred into the F1 and backcross derivatives. The new wheat-Ae. sharonensis derivatives that we have produced will be valuable for increasing resistance to various diseases of wheat and for improving the quality of bread wheat.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)