Cereal Research Communications
Volume/Issue:
Volume 42: Issue 4
A new HMW-GS 1Bx23* containing an amino acid segment similar to collagen
Authors: L. Wei, S.G. Bai 4 , X.J. Hou 2 , J.M. Li 2 , B. Zhang, W.J. Chen, D.C. Liu, B.L. Liu and H.G. Zhang
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  • 1 Chinese Academy of Sciences Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology Qinghai Xining 810001 China
  • 2 Qinghai Normal University College of Biologic and Geographic Sciences Qinghai Xining 810001 China
  • 3 Qinghai Province Key Laboratory of Crop Molecular Breeding Xining 810001 China
  • 4 Grassland Station of Huangzhong County Xining 811600 China
  • 5 State Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm Xining 810001 China
DOI:
https://doi.org/10.1556/crc.2014.0012
Page Count:
658–667
Publication Date:
01 Dec 2014
Online Publication Date:
15 Jun 2014
Article Category:
Research Article
Restricted access

Among 20 awnless Tibetan wheat cultivars analyzed by SDS-PAGE, the migration rate of an HMW-GS in XM001584 and XM001593, named 1BX23*. was shown to be slightly faster than 1Bx6. and slower than Bx7. Its nucleotide sequence was isolated based on homology clones. In a phylogenetic tree of 1Bx genes, 1Bx23* was apparently clustered with 1Bx23. Compared with 1Bx23. eight single nucleotide replacements caused four single amino acid replacements in 1Bx23*. The deletion of “G” at base pair 1463 and insertion of “A” at 1509 bps induced a 42-nucleotide frame shift. “GQRQQAGQWQRPGQ” was replaced by “DKGNRQDNGNDRDK”. The new segment cannot be found in other HMW-GSs, and it is very similar to a segment found in collagen. Moreover, an 18-nucleotide deletion made 1Bx23* six amino acids shorter than 1Bx23. The cultivar XM001593 had 28 chromosomes, which signifies that it was tetraploid wheat, and that the new HMW-GS 1Bx23* cannot be used directly for breeding in common wheat.

  • Anjum F.M., Khan M.R., Din A., Saeed M., Pasha I., Arshad M.U. 2007. Wheat gluten: High molecular weight glutenin subunits — Structure, genetics, and relation to dough elasticity. J. Food Sci. 72:R56–R63.

    Arshad M.U. , 'Wheat gluten: High molecular weight glutenin subunits — Structure, genetics, and relation to dough elasticity ' (2007 ) 72 J. Food Sci. : R56 -R63.

    • Search Google Scholar

  • Buonocore F., Bertin L., Ronchi C., Bekes F., Caporale C., Lafiandra D., Gras P., Tatham A.S., Greenfield J.A., Halford N.G. 1998. Expression and functional analysis of M-r 58 000 peptides derived from the repetitive domain of high molecular weight glutenin subunit 1 Dx5. J. Cereal Sci. 27:209–215.

    Halford N.G. , 'Expression and functional analysis of M-r 58 000 peptides derived from the repetitive domain of high molecular weight glutenin subunit 1 Dx5 ' (1998 ) 27 J. Cereal Sci. : 209 -215.

    • Search Google Scholar

  • Harberd N.P., Bartels D., Thompson R.D. 1986. DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochem. Genet. 24:579–596.

    Thompson R.D. , 'DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat ' (1986 ) 24 Biochem. Genet. : 579 -596.

    • Search Google Scholar

  • Juhasz A., Tamas L., Karsai I., Vida G., Lang L., Bedo Z. 2001. Identification, cloning and characterisation of a HMW-glutenin gene from an old Hungarian wheat variety, Bankuti 1201. Euphytica 119:75–79.

    Bedo Z. , 'Identification, cloning and characterisation of a HMW-glutenin gene from an old Hungarian wheat variety, Bankuti 1201 ' (2001 ) 119 Euphytica : 75 -79.

    • Search Google Scholar

  • Kumar S., Nei M., Dudley J., Tamura K. 2008. MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform. 9:299–306.

    Tamura K. , 'MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences ' (2008 ) 9 Brief Bioinform. : 299 -306.

    • Search Google Scholar

  • Li X., Zhang Y., Gao L., Wang A., Ji K., He Z., Appels R., Ma W., Yan Y. 2007. Molecular cloning, heterologous expression, and phylogenetic analysis of a novel y-typeHMWglutenin subunit gene from the G genome of Triticum timopheevi. Genome 50:1130–1140.

    Yan Y. , 'Molecular cloning, heterologous expression, and phylogenetic analysis of a novel y-typeHMWglutenin subunit gene from the G genome of Triticum timopheevi ' (2007 ) 50 Genome : 1130 -1140.

    • Search Google Scholar

  • Liu S.W., Gao X., Xia G.M. 2008. Characterizing HMW-GS alleles of decaploid Agropyron elongatum in relation to evolution and wheat breeding. Theor. Appl. Genet. 116:325–334.

    Xia G.M. , 'Characterizing HMW-GS alleles of decaploid Agropyron elongatum in relation to evolution and wheat breeding ' (2008 ) 116 Theor. Appl. Genet. : 325 -334.

    • Search Google Scholar

  • Payne, PI. 1987. Genetics of wheat storage proteins and the effect of allelic variation on bread making quality. Annu. Rev. Plant Physiol. 38:141–153.

    Payne P.I. , 'Genetics of wheat storage proteins and the effect of allelic variation on bread making quality ' (1987 ) 38 Annu. Rev. Plant Physiol. : 141 -153.

    • Search Google Scholar

  • Shewry P.R., Halford N.G. 2003. Genetics of wheat gluten proteins. Advances in Genetics 49:111–184.

    Halford N.G. , 'Genetics of wheat gluten proteins ' (2003 ) 49 Advances in Genetics : 111 -184.

    • Search Google Scholar

  • Shewry P.R., Tatham A.S. 1997. Disulphide bonds in wheat gluten proteins. J. Cereal Sci. 25:207–227.

    Tatham A.S. , 'Disulphide bonds in wheat gluten proteins ' (1997 ) 25 J. Cereal Sci. : 207 -227.

    • Search Google Scholar

  • Shewry P., Halford N., Tatham Yves A. 2003. The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv. Food Nutr. Res. 45:219–302.

    Tatham Yves A. , 'The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties ' (2003 ) 45 Adv. Food Nutr. Res. : 219 -302.

    • Search Google Scholar

  • Tatham A.S., Drake A.F., Shewry P.R. 1990. Conformational studies of synthetic peptides corresponding to the repetitive regions of the high-molecular-weight (Hmw) glutenin subunits of wheat. J. Cereal Sci. 11:189–200.

    Shewry P.R. , 'Conformational studies of synthetic peptides corresponding to the repetitive regions of the high-molecular-weight (Hmw) glutenin subunits of wheat ' (1990 ) 11 J. Cereal Sci. : 189 -200.

    • Search Google Scholar

  • Tatham A.S., Miflin B.J., Shewry P.R. 1985. The beta-turn conformation in wheat gluten proteins — Relationship to gluten elasticity. Cereal Chem. 62:405–412.

    Shewry P.R. , 'The beta-turn conformation in wheat gluten proteins — Relationship to gluten elasticity ' (1985 ) 62 Cereal Chem. : 405 -412.

    • Search Google Scholar

  • Tilley K.A., Benjamin R.E., Bagorogoza K.E., Okot-Kotber B.M., Prakash O., Kwen H. 2001. Tyrosine cross-links: Molecular basis of gluten structure and function. J. of Agricultural and Food Chem. 49:2627–2632.

    Kwen H. , 'Tyrosine cross-links: Molecular basis of gluten structure and function ' (2001 ) 49 J. of Agricultural and Food Chem. : 2627 -2632.

    • Search Google Scholar

  • Yan Y.M., Jiang Y., An X.L., Pei Y.H., Li X.H., Zhang Y.Z., Wang A.L., He Z., Xia X., Bekes F. 2009. Cloning, expression and functional analysis of HMW glutenin subunit 1By8 gene from Italy pasta wheat (Triticum turgidum L. ssp. durum). J. Cereal Sci. 50:398–406.

    Bekes F. , 'Cloning, expression and functional analysis of HMW glutenin subunit 1By8 gene from Italy pasta wheat (Triticum turgidum L. ssp. durum) ' (2009 ) 50 J. Cereal Sci. : 398 -406.

    • Search Google Scholar

  • Yang Z.J., Li G.R., Shu H.L., Liu C., Feng J., Chang Z.J., Ren Z.L. 2006. Molecular characterization of high molecular weight glutenin subunit allele 1Bx23 in common wheat introduced from hexaploid triticale. Hereditas 143:159–166.

    Ren Z.L. , 'Molecular characterization of high molecular weight glutenin subunit allele 1Bx23 in common wheat introduced from hexaploid triticale ' (2006 ) 143 Hereditas : 159 -166.

    • Search Google Scholar
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170
Keywords:
Tibetan wheat cultivar; HMW-GS; Glu-Bx; allelic variation

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