Molecular evidence of the haploid origin in wheat (Triticum aestivum L.) with Aegilops kotschyi cytoplasm and whole genome expression profiling after haploidization

J.L. Wang; Y.N. Wang; D.C. Liu; X.L. Guo; W.L. Yang; K.H. Zhan; X.J. Wang; A.M. Zhang

doi:10.1556/crc.2014.0024

Cereal Research Communications

Volume/Issue:: Volume 43: Issue 1

Molecular evidence of the haploid origin in wheat (Triticum aestivum L.) with Aegilops kotschyi cytoplasm and whole genome expression profiling after haploidization

Authors:

J.L. Wang

J.L. Wang

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Y.N. Wang

Y.N. Wang CAS State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology Beijing 100101 China

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D.C. Liu

D.C. Liu CAS State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology Beijing 100101 China

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X.L. Guo

X.L. Guo China Agricultural University College of Biology Beijing 100193 China

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W.L. Yang

W.L. Yang CAS State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology Beijing 100101 China

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K.H. Zhan

K.H. Zhan Henan Agricultural University The Collaborative Innovation Center for Grain Crops in Henan, College of Agronomy Zhengzhou 450002 China

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X.J. Wang

X.J. Wang CAS State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology Beijing 100101 China

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A.M. Zhang

A.M. Zhang

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Pages:: 1–11

Online Publication Date:: 25 Sep 2014

Publication Date:: 01 Mar 2015

Article Category:: Research Article

DOI:: https://doi.org/10.1556/crc.2014.0024

Keywords:: wheat; microsatellite; hexaploid; haploid; gene chip

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Aegiolops kotschyi cytoplasmic male sterile system often results in part of haploid plants in wheat (Triticum aestivum L.). To elucidate the origin of haploid, 235 wheat microsatellite (SSR) primers were randomly selected and screened for polymorphism between haploid (2n = 3x = 21 ABD) and its parents, male-sterile line YM21 (2n = 6x = 42 AABBDD) and male fertile restorer YM2 (2n = 6x = 42 AABBDD). About 200 SSR markers yielded clear bands from denatured PAGE, of which 180 markers have identifiable amplification patterns, and 20 markers (around 8%) resulted in different amplification products between the haploid and the restorer, YM2. There were no SSR markers that were found to be distinguishable between the haploid and the male sterile line YM21. In addition, different distribution of HMW-GS between endosperm and seedlings from the same seeds further confirmed that the haploid genomes were inherited from the maternal parent. After haploidization, 1.7% and 0.91% of total sites were up- and down-regulated exceeding twofold in the shoot and the root of haploid, respectively, and most of the differentially expressed loci were up/down-regulated about twofold. Out of the sensitive loci in haploid, 94 loci in the shoot, 72 loci in the root can be classified into three functional subdivisions: biological process, cellular component and molecular function, respectively.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
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ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)