Celiac disease-specific prolamin peptide content of wheat relatives and wild species determined by ELISA assays and bioinformatics analyses

Gy. Gell; K. Kovács; I. Molnár; Zs. Bugyi; S. Tömösközi; A. Juhász

doi:10.1556/crc.43.2015.1.13

Cereal Research Communications

Volume/Issue:: Volume 43: Issue 1

Celiac disease-specific prolamin peptide content of wheat relatives and wild species determined by ELISA assays and bioinformatics analyses

Authors:

Gy. Gell

Gy. Gell HAS Applied Genomics Department, Agricultural Institute, Centre for Agricultural Research Martonvásár Hungary

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K. Kovács

K. Kovács HAS Applied Genomics Department, Agricultural Institute, Centre for Agricultural Research Martonvásár Hungary

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I. Molnár

I. Molnár HAS Department of Plant Genetic Resources and Organic Breeding, Agricultural Institute, Centre for Agricultural Research Martonvásár Hungary

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Zs. Bugyi

Zs. Bugyi Budapest University of Technology and Economics Department of Applied Biotechnology and Food Science Budapest Hungary

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S. Tömösközi

S. Tömösközi Budapest University of Technology and Economics Department of Applied Biotechnology and Food Science Budapest Hungary

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, and

A. Juhász

A. Juhász HAS Applied Genomics Department, Agricultural Institute, Centre for Agricultural Research Martonvásár Hungary

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Pages:: 133–143

Online Publication Date:: 09 Feb 2015

Publication Date:: 01 Mar 2015

Article Category:: Research Article

DOI:: https://doi.org/10.1556/crc.43.2015.1.13

Keywords:: ELISA; R5 epitope; G12 epitope; celiac disease; cereal species

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Enzyme-linked immunosorbent assays (ELISAs) are widely used to determine gluten contamination in gluten-free and low gluten food samples. ELISA assays developed using monoclonal antibodies against known toxic peptides have an advantage in the identification of toxic prolamin content in protein extracts of different food samples, as well as raw materials. R5 and G12 monoclonal antibodies specific for two known toxic peptides used in commercially available gluten ELISA assays were applied to test toxic peptide contents in wheat relatives and wild wheat species with different genome composition and complexity. Although the R5 peptide content showed some correlation with ploidy levels in Triticum species, there was a high variance among Aegilops species. Some of the analysed diploid Aegilops species showed extremely high R5 peptide contents. Based on the bioinformatics analyses, the R5 peptide was present in most of the sulphur rich prolamins in all the analysed species, whereas the G12 epitope was exclusively present in alpha gliadins. High variation was detected in the position and frequency of epitopes in sequences originating from the same species, thus highlighting the importance of genotypic variation within species. Identification of new prolamin alleles of wheat relatives and wild wheat species is of great importance in order to find germplasm for special end-use quality purposes as well as development of food with reduced toxicity.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)