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  • 1 Institute of Field and Vegetable Crops 30 Maksima Gorkog 21000 Novi Sad Serbia
  • | 2 Biogranum Novi Sad Serbia
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The microsatellites, as one of the most robust markers for identification of wheat varieties, were used for assessment of genetic diversity and population structure to promote effective use of genetic resources. In this study, the set of 284 wheat varieties were genotyped using 30 microsatellite markers. The chosen SSR markers were located among almost all linkage groups and covered all three genomes. The genotypes used originate from 24 different breeding centers worldwide and are included in an extensive core collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. The total number of detected alleles was 349 at all analyzed loci. The average number of detected allelic variant per locus was 11.5. The mean value of polymorphic information content was 0.68. According to the probability of data obtained by program Structure, the results have shown presence of 6 subpopulations within the studied set of genotypes. The population structure positively correlated to some extent with geographic origin. The available pedigree data were included for additional explanation of population structure. The results of this study should provide valuable information for future association studies using the diverse wheat breeding material.

  • Borojevic, K., Borojevic, K. 2005. Historic role of the wheat variety Akakomughi in Southern and Central European wheat breeding programs. Breeding Sci. 55:253–256.

    Borojevic K. , 'Historic role of the wheat variety Akakomughi in Southern and Central European wheat breeding programs ' (2005 ) 55 Breeding Sci. : 253 -256.

    • Search Google Scholar
  • Chao, S.M., Zhang, W.J., Dubcovsky, J., Sorrels, M. 2007. Evaluation of genetic diversity and genome-wide likage disequilibrium among U.S. wheat (Triticum aestivum L.) germplasm representing different market classes. Crop Sci. 47:1018–1030.

    Sorrels M. , 'Evaluation of genetic diversity and genome-wide likage disequilibrium among U.S. wheat (Triticum aestivum L.) germplasm representing different market classes ' (2007 ) 47 Crop Sci. : 1018 -1030.

    • Search Google Scholar
  • Chen, X., Min, D., Yasir, T.A., Hu, Y.G. 2012. Genetic diversity, population structure and linkage disequilibrium in elite Chinese winter wheat investigated with SSR markers. PLoS one 7(9):e44510. doi: 10.1371/journal.pone 0044510

    Hu Y.G. , 'Genetic diversity, population structure and linkage disequilibrium in elite Chinese winter wheat investigated with SSR markers ' (2012 ) 7 PLoS one : e44510 -.

    • Search Google Scholar
  • Chen, X.M., He, Z.H., Shi, J.R., Xia, L.Q., Ward, R., Zhou, Y., Jiang, G.L. 2003. Genetic diversity of high quality winter wheat varieties (lines) based on SSR marker. Acta Agronomica Sinica 29:13–19.

    Jiang G.L. , 'Genetic diversity of high quality winter wheat varieties (lines) based on SSR marker ' (2003 ) 29 Acta Agronomica Sinica : 13 -19.

    • Search Google Scholar
  • Couviour, F.L., Faure, S., Poupard, B., Flodrops, Y., Dubreuil, P., Praud, S. 2011. Analysis of genetic structure in a panel of elite wheat varieties and relevance for association mapping. Theor. Appl. Genet. 123:715–727.

    Praud S. , 'Analysis of genetic structure in a panel of elite wheat varieties and relevance for association mapping ' (2011 ) 123 Theor. Appl. Genet. : 715 -727.

    • Search Google Scholar
  • Doyle, J.J., Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13–15.

    Doyle J.L. , 'Isolation of plant DNA from fresh tissue ' (1990 ) 12 Focus : 13 -15.

  • Flint-Garcia, S.A., Thornsberry, J.M., Buckler, E.S. 4th 2003. Structure of linkage disequilibrium in plants. Annu. Rev. Plant Biol. 54:357–374.

    Buckler E.S. , 'Structure of linkage disequilibrium in plants ' (2003 ) 54 Annu. Rev. Plant Biol. : 357 -374.

    • Search Google Scholar
  • Huang, X.Q., Börner, A., Röder, M.S., Ganal, M.W. 2002. Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Theor. Appl. Genet. 105:699–707.

    Ganal M.W. , 'Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers ' (2002 ) 105 Theor. Appl. Genet. : 699 -707.

    • Search Google Scholar
  • Kobiljski, B., Dencic, S., Kondic-Spika, A., Lohwasser, U., Börner, A. 2009. Locating stable across environment QTL involved in the determination of agronomic characters in wheat. Cereal Res. Commun. 37:327–333.

    Börner A. , 'Locating stable across environment QTL involved in the determination of agronomic characters in wheat ' (2009 ) 37 Cereal Res. Commun. : 327 -333.

    • Search Google Scholar
  • Liu, K., Muse, S.V. 2005. PowerMarker: An integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129.

    Muse S.V. , 'PowerMarker: An integrated analysis environment for genetic marker analysis ' (2005 ) 21 Bioinformatics : 2128 -2129.

    • Search Google Scholar
  • Neumann, K., Kobiljski, B., Dencic, S., Varshney, R.K., Börner, A. 2011. Genome-wide association mapping: A case study in bread wheat (Triticum aestivum L.). Mol. Breed. 27:37–58.

    Börner A. , 'Genome-wide association mapping: A case study in bread wheat (Triticum aestivum L.) ' (2011 ) 27 Mol. Breed. : 37 -58.

    • Search Google Scholar
  • Pritchard, J.K., Stephens, M., Donnelly, P. 2000. Inference of population structure using multilocus genotype data. Genetics 155:945–959.

    Donnelly P. , 'Inference of population structure using multilocus genotype data ' (2000 ) 155 Genetics : 945 -959.

    • Search Google Scholar
  • Randhawa, H.S., Muhammad, A., Pozniak, C., Clarke, J.M., Graf, R.J., Fox, S.L., Humphreys, D.G., Knox, R.E., Depauw, R.M., Singh, A.K., Cuthbert, R.D., Hucl, P., Spaner, D. 2013. Application of molecular markers to wheat breeding in Canada. Plant Breed. 132:458–471.

    Spaner D. , 'Application of molecular markers to wheat breeding in Canada ' (2013 ) 132 Plant Breed. : 458 -471.

    • Search Google Scholar
  • Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M-H., Leroz, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 194:2007–2023.

    Ganal M.W. , 'A microsatellite map of wheat ' (1998 ) 194 Genetics : 2007 -2023.

  • Röder, M.S., Wendehake, K., Korzun, V., Bredemeijer, G., Laborie, D., Bertrand, L., Isaac, P., Rendell, S., Jackson, J., Cooke, R.J., Vosman, B., Ganal, M. 2002. Construction and analysis of a microsatellite-based database of European wheat varieties. Theor. Appl. Genet. 106:67–73.

    Ganal M. , 'Construction and analysis of a microsatellite-based database of European wheat varieties ' (2002 ) 106 Theor. Appl. Genet. : 67 -73.

    • Search Google Scholar
  • Stachel, M., Lelley, T., Grausgruber, H., Vollmann, J. 2000. Application of microsatellites in wheat (Triticum aestivum L.) for studying genetic differention caused by selection for adaptation and use. Theor. Appl. Genet.100:242–248.

    Vollmann J. , 'Application of microsatellites in wheat (Triticum aestivum L.) for studying genetic differention caused by selection for adaptation and use ' (2000 ) 100 Theor. Appl. Genet. : 242 -248.

    • Search Google Scholar
  • Varshney, R.K., Thudi, M., Aggarwal, R., Börner, A. 2007. Genic molecular markers in plants: Development and applications. In: Varshney, R.K., Tuberosa, R. (eds), Genomics-assisted Crop Improvement: Genomics Approaches and Platforms. Vol. 1. Springer, Dordrecht, The Netherlands, pp. 13–29.

    Börner A. , '', in Genomics-assisted Crop Improvement: Genomics Approaches and Platforms , (2007 ) -.

  • Wang, Y., Wang, C., Zhang, H., Yue, Z., Liu, X., Ji, W. 2013. Genetic analysis of wheat (Triticum aestivum L.) and related species with SSR markers. Genetic Res. and Crop Evol. 60:1105–1117.

    Ji W. , 'Genetic analysis of wheat (Triticum aestivum L.) and related species with SSR markers ' (2013 ) 60 Genetic Res. and Crop Evol. : 1105 -1117.

    • Search Google Scholar
  • Yu, J., Buckler, E.S. 2006. Genetic association mapping and genome organization of maize. Current Opinion in Biotechnol. 17:155–160.

    Buckler E.S. , 'Genetic association mapping and genome organization of maize ' (2006 ) 17 Current Opinion in Biotechnol. : 155 -160.

    • Search Google Scholar
  • Zhang, D., Bai, G., Zhu, C., Yu, J., Carver, B.F. 2010. Genetic diversity, population structure, and linkage disequilibrium in U.S. elite winter wheat. The Plant Genome 3:117–127.

    Carver B.F. , 'Genetic diversity, population structure, and linkage disequilibrium in U.S. elite winter wheat ' (2010 ) 3 The Plant Genome : 117 -127.

    • Search Google Scholar
  • Zhang, L., Liu, D.C., Guo, X.L., Yang, W.L., Sun, J.Z., Wang, D.W., Sourdille, P., Zhang, A.M. 2011. Investigation of genetic diversity and population structure of common wheat cultivars in northern China using DArT markers. BMC Genetics 12:42.

    Zhang A.M. , 'Investigation of genetic diversity and population structure of common wheat cultivars in northern China using DArT markers ' (2011 ) 12 BMC Genetics : 42 -.

    • Search Google Scholar