Cereal Research Communications
Volume/Issue:
Volume 43: Issue 2
The Analysis of SKP1 Gene Expression in Physiological Male Sterility Induced by Chemical Hybridizing Agent SQ-1 in Wheat (Triticum aestivum L.)
Authors:
M.Y. Wang Key Laboratory of Crop Heterosis of Shaanxi Province / National Yangling Agricultural Biotechnology and Breeding Center / Yangling Branch of State Wheat Improvement Centre / Wheat Breeding Engineering Research Center, Ministry of Education / Northwest A&F University, Yangling, Shaanxi, 712100, PR China

Search for other papers by M.Y. Wang in
Current site
Google Scholar
PubMed Close
,
Y.L. Song Key Laboratory of Crop Heterosis of Shaanxi Province / National Yangling Agricultural Biotechnology and Breeding Center / Yangling Branch of State Wheat Improvement Centre / Wheat Breeding Engineering Research Center, Ministry of Education / Northwest A&F University, Yangling, Shaanxi, 712100, PR China

Search for other papers by Y.L. Song in
Current site
Google Scholar
PubMed Close
,
S.X. Zhang Institute of Crop Science, Ningxia Academy of Agricultural Sciences, Yongning, 750105, Ningxia, PR China

Search for other papers by S.X. Zhang in
Current site
Google Scholar
PubMed Close
,
X.L. Zhao Key Laboratory of Crop Heterosis of Shaanxi Province / National Yangling Agricultural Biotechnology and Breeding Center / Yangling Branch of State Wheat Improvement Centre / Wheat Breeding Engineering Research Center, Ministry of Education / Northwest A&F University, Yangling, Shaanxi, 712100, PR China

Search for other papers by X.L. Zhao in
Current site
Google Scholar
PubMed Close
,
J.W. Wang Key Laboratory of Crop Heterosis of Shaanxi Province / National Yangling Agricultural Biotechnology and Breeding Center / Yangling Branch of State Wheat Improvement Centre / Wheat Breeding Engineering Research Center, Ministry of Education / Northwest A&F University, Yangling, Shaanxi, 712100, PR China

Search for other papers by J.W. Wang in
Current site
Google Scholar
PubMed Close
,
N. Niu Key Laboratory of Crop Heterosis of Shaanxi Province / National Yangling Agricultural Biotechnology and Breeding Center / Yangling Branch of State Wheat Improvement Centre / Wheat Breeding Engineering Research Center, Ministry of Education / Northwest A&F University, Yangling, Shaanxi, 712100, PR China

Search for other papers by N. Niu in
Current site
Google Scholar
PubMed Close
, and
G.S. Zhang Key Laboratory of Crop Heterosis of Shaanxi Province / National Yangling Agricultural Biotechnology and Breeding Center / Yangling Branch of State Wheat Improvement Centre / Wheat Breeding Engineering Research Center, Ministry of Education / Northwest A&F University, Yangling, Shaanxi, 712100, PR China

Search for other papers by G.S. Zhang in
Current site
Google Scholar
PubMed Close
Pages:
204–212
Online Publication Date:
Jun 2015
Publication Date:
01 Jun 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/crc.43.2015.2.3
Keywords:
wheat; CHA; tapetum; RT-PCR
Restricted access

Physiological male sterility induced by the chemical hybridizing agent (CHA) overcomes problems of maintenance of sterile lines and restorers. However, the mechanism of sterility is unclear. The process of tapetum of CHA-treated ‘Xi’nong 2611’ at uninucleate, binucleate and trinucleate were compared with control to determine if tapetum varying differently during developmental stages. Tapetal degradation in CHA-treated ‘Xi’nong 2611’ began at late uninucleate stage, somewhat earlier than control plants. Cytological observations indicated that the gradual degradation of the tapetum in CHA-treated ‘Xi’nong 2611’ was initiated and terminated earlier than in the control. These findings implied that CHA-induced male sterility was related to abnormally early tapetal degradation. In order to indicate the role of the SKP1 gene in fertility/sterility in wheat, its expression was assessed in anthers at uninucleate, binucleate and trinucleate stages. SKP1 expression was reduced in the later developmental stages, and there was an obvious decrease from the uninucleate to trinucleate stages. Higher expression of the SKP1 gene occurred in ‘Xi’nong 2611’ compared to CHA-treated ‘Xi’nong 2611’. This implied that SKP1 gene expression was inhibited during the fertility transformation process and was related to transformation from fertility to sterility. Moreover, the results from this study suggest that SKP1 plays an essential role of conducting fertility in physiological male sterility.

  • Aarts, M.G., Hodge, R., Kalantidis, K., Florack, D., Wilson, Z.A., Mulligan, B.J., Stiekema, W.J., Scott, R., Pereira, A. 1997. The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes. The Plant Journal 12:615–623.

  • Ba, Q.S., Zhang, G.S., Wang, J.S., Niu, N., Ma, S.C., Wang, J.W. 2014. Gene expression and DNA methylation alterations in chemically induced male sterility anthers in wheat (Triticum aestivum L.). Acta Physiol. Plant. 36:503–512.

    • Search Google Scholar
    • Export Citation

  • Bai, C., Sen, P., Hofmann, K., Ma, L., Goebl, M., Harper, J.W., Elledge, S.J. 1996. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86:263–274.

    • Search Google Scholar
    • Export Citation

  • Chai, L.J., Biswas, M.K., Ge, X.X., Deng, X.X. 2010. Isolation, characterization, and expression analysis of an SKP1-like gene from ‘Shatian’ pummelo (Citrus grandis Osbeck). Plant Mol. Biol. Rep. 28:569–577.

    • Search Google Scholar
    • Export Citation

  • Chai, L.J., Ge, X.X., Biswas, M.K., Deng, X.X. 2011. Molecular analysis and expression of a floral organ-relative F-box gene isolated from ‘Zigui shatian’ pummel (Citrus grandis Osbeck). Mol. Biol. Rep. 38:4429–4436.

    • Search Google Scholar
    • Export Citation

  • Cheng, C.S., Lu, Y.F., Zhang, G.S., Zhang, Z.J., Li, P., Zhang, B.Y. 2004. The synthesis and biological of lamicital acid on chemical hybridizing agent. Pepsiticides 43:315–317. (In Chinese with English abstract)

    • Search Google Scholar
    • Export Citation

  • Drouaud, J., Marrocco, K., Ridel, C., Pelletier, G., Guerche, P. 2000. A Brassica napus skp1-like gene promoter drives GUS expression in Arabidopsis thaliana male and female gametophytes. Sex. Plant Reprod. 13:29–35.

    • Search Google Scholar
    • Export Citation

  • Farras, R., Ferrando, A., Jasik, J., Kleinow, T., Okresz, L., Tiburcio, A., Salchert, K., del Pozo, C., Schell, J., Koncz, C. 2001. SKP1-SnRK protein kinase interactions mediate proteasomal binding of a plant SCF ubiquitin ligase. EMBO J. 20:2742–2756.

    • Search Google Scholar
    • Export Citation

  • Gagne, J.M., Downes, B.P., Shiu, S.H., Durski, A.M., Vierstra, R.D. 2002. The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis. Proc. Natl Acad. Sci. USA 99:11519–11524.

    • Search Google Scholar
    • Export Citation

  • Hellmann, H., Estelle, M. 2002. Plant development: regulation by protein degradation. Science 297:793–797.

  • Hershko, A., Heller, H., Elias, S., Ciechanover, A. 1983. Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown. J. Biol. Chem. 258:8206–8214.

    • Search Google Scholar
    • Export Citation

  • Hershko, A., Ciechanover, A. 1998. The ubiquitin system. Annu. Rev. Biochem. 67:425–479.

  • Hong, M.J., Kim, D.Y., Seo, Y.W. 2013. SKP1-like-related genes interact with various F-box proteins and may form SCF complexes with Cullin–F-box proteins in wheat. Mol. Biol. Rep. 40:969–981.

    • Search Google Scholar
    • Export Citation

  • Kong, H.Z., Leebens-Mack, J., Ni, W., dePamphilis, C.W., Ma, H. 2004. Highly heterogeneous rates of evolution in the SKP1 gene family in plants and animals: Functional and evolutionary implications. Mol. Biol. and Evol. 21:117–128.

    • Search Google Scholar
    • Export Citation

  • Kong, H.Z., Landherr, L.L., Frohlich, M.W., Leebens-Mack, J., Ma, H., dePamphilis, C.W. 2007. Patterns of gene duplication in the plant SKP1 gene family in angiosperms: Evidence for multiple mechanisms of rapid gene birth. Plant J. 50:873–885.

    • Search Google Scholar
    • Export Citation

  • Li, C.J., Liang, Y., Chen, C.B., Li, J.H., Xu, Y.Y., Xu, Z.H., Ma, H., Chong, K. 2006. Cloning and expression analysis of TSK1, a wheat SKP1 homologue, and functional comparison with Arabidopsis ASK1 in male meiosis and auxin signaling. Functional Plant Biol. 33:381–390.

    • Search Google Scholar
    • Export Citation

  • Liu, H.W., Zhang, G.S., Wang, J.W., Wang, X.L., Fang, Z.W. 2003. Effect of male sterility on different wheat genotype induced by SQ-1. Northwest Univ. Agric. Forestry Nat. Sci. 31:15–18. (In Chinese with English abstract)

    • Search Google Scholar
    • Export Citation

  • Liu, H.Z., Zhang, G.S., Zhu, W.W., Wu, W.K.K., Ba, Q.S., Zhang, L., Zhang, L.Y., Niu, N., Ma, S.C., Wang, J.W. 2014. Differential proteomic analysis of polyubiquitin-related proteins in chemical hybridization agent-induced wheat (Triticum aestivum L.) male sterility. Acta Physiol. Plant. 36:1473–1489.

    • Search Google Scholar
    • Export Citation

  • Marrocco, K., Lecureuil, A., Nicolas, P., Guerche, P. 2003. The Arabidopsis SKP1-like genes present a spectrum of expression profiles. Plant Mol. Biol. 52:715–727.

    • Search Google Scholar
    • Export Citation

  • Murray, M.G., Thompson, W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8:4321–43254.

  • Pickart, C.M. 2001. Mechanisms underlying ubiquitination. Annu. Rev. Biochem. 70:503–533.

  • Risseeuw, E.P., Daskalchuk, T.E., Banks, T.W., Liu, E., Cotelesage, J., Hellmann, H., Estelle, M., Somers, D.E., Crosby, W.L. 2003. Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis. Plant J. 34:753–767.

    • Search Google Scholar
    • Export Citation

  • Santner, A., Estelle, M. 2010. The ubiquitin-proteasome system regulates plant hormone signaling. Plant J. 61:1029–1040.

  • Sasaki, A., Itoh, H., Gomi, K., Ueguchi-Tanaka, M., Ishiyama, K. 2003. Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant. Science 299:1896–1898.

    • Search Google Scholar
    • Export Citation

  • Sheng, Y., Zhang, G.S., Li, Y.X., Zhang, L.Y., Wang, S.P., Zhao, X.L., Wang, L.M., Song, Y.L. 2011. The relationship of anther tapetum, sporopollenin and expression of RAFTIN1 in physiological male sterile wheat. Scientia Agricultura Sinica 44:3937–3944. (In Chinese with English abstract)

    • Search Google Scholar
    • Export Citation

  • Smalle, J., Vierstra, R.D. 2004. The ubiquitin 26S proteasome preteolytic pathway. Annu. Rev. Plant Biol. 55:555–590.

  • Stone, S.L., Callins, J. 2007. Ubiquitin ligases mediate growth and development by promoting protein death. Curr. Opin. Plant Biol. 10:624–632.

    • Search Google Scholar
    • Export Citation

  • Takahashi, N., Kuroda, H., Kuromori, T., Hirayama, T., Seki, M., Shinozaki, K., Shimada, H., Matsui, M. 2004. Expression and interaction analysis of Arabidopsis Skp1-related genes. Plant Cell Physiol. 45:83–91.

    • Search Google Scholar
    • Export Citation

  • Taylor, P.E., Glover, J.A., Lavithis, M., Craig, S., Singh, M.B., Knox, R.B., Dennis, E.S., Chaudhury, A.M. 1998. Genetic control of male fertility in Arabidopsis thaliana. Structural analyses of postmeiotic developmental mutants. Planta 205:492–505.

    • Search Google Scholar
    • Export Citation

  • Wang, F., Deng, X.W. 2011. Plant ubiquitin-proteasome pathway and its role in gibberellin signaling. Cell Res. 21:1286–1294.

  • Yang, M., Hu, Y., Lodhi, M., McCombie, W.R., Ma, H. 1999. The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation. Proc. Natl Acad. Sci. USA 96:11416–11421.

    • Search Google Scholar
    • Export Citation

  • Zhang, C.Y., Xu, S.G., Huang, X.X. 2005. A novel and convenient relative quantitative method of fluorescence real time RT-PCR assay based on slope of standard curve. Prog. Biochem. Biophys. 9:883–887. (In Chinese with English abstract)

    • Search Google Scholar
    • Export Citation

  • Zhao, D., Han, T., Risseeuw, E., Crosby, W.L., Ma, H. 2003a. Conservation and divergence of ASK1 and ASK2 gene functions during male meiosis in Arabidopsis thaliana. Plant Mol. Biol. 53:163–173.

    • Search Google Scholar
    • Export Citation

  • Zhao, D., Ni, W., Feng, B., Han, T., Petrasek, M.G., Ma, H. 2003b. Members of the Arabidopsis-SKP1-like gene family exhibit a variety of expression patterns and may play diverse roles in Arabidopsis. Plant Physiol. 133:203–217.

    • Search Google Scholar
    • Export Citation
  • Collapse
  • Expand
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

 

 

For subscription options, please visit the website of Springer Nature.

Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Mar 2024 20 1 0
Apr 2024 14 0 0
May 2024 10 0 0
Jun 2024 27 0 0
Jul 2024 47 0 0
Aug 2024 43 1 0
Sep 2024 6 0 0