In silico cloning and chromosomal localization of EST sequences that are related to leaf senescence using nulli-tetrasomes in wheat

J.W Guo; Q. Li; W.Q. Chen; X. Li; L.Q. Li; T.G. Liu; Z.L. Ren; P.G. Luo

doi:10.1556/0806.43.2015.014

Cereal Research Communications

Volume/Issue:: Volume 43: Issue 3

In silico cloning and chromosomal localization of EST sequences that are related to leaf senescence using nulli-tetrasomes in wheat

Authors:

J.W Guo

J.W Guo Sichuan Agricultural University, Chengdu, Sichuan 611130, China
Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China

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Q. Li

Q. Li Sichuan Agricultural University, Chengdu, Sichuan 611130, China

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W.Q. Chen

W.Q. Chen Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China

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X. Li

X. Li Sichuan Agricultural University, Chengdu, Sichuan 611130, China

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L.Q. Li

L.Q. Li Sichuan Agricultural University, Chengdu, Sichuan 611130, China

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T.G. Liu

T.G. Liu Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China

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Z.L. Ren

Z.L. Ren Sichuan Agricultural University, Chengdu, Sichuan 611130, China

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P.G. Luo

P.G. Luo Sichuan Agricultural University, Chengdu, Sichuan 611130, China
Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China

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Pages:: 364–373

Online Publication Date:: Sep 2015

Publication Date:: 01 Sep 2015

Article Category:: Research Article

DOI:: https://doi.org/10.1556/0806.43.2015.014

Keywords:: expression sequence tag (EST); leaf senescence; nullisomic-tetrasomic; in silico mapping; wheat

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Leaf senescence is a notably important trait that limits the yield and biomass accumulation of agronomic crops. Therefore, determining the chromosomal position of the expression sequence tags (ESTs) that are associated with leaf senescence is notably interesting in the manipulation of leaf senescence for crop improvement. A total of 32 ESTs that were previously identified during the delaying leaf senescence stage in the stay-green wheat cultivar CN17 were mapped to 42 chromosomes, a chloroplast, a mitochondrion, and a ribosome using in silico mapping. Then, we developed 19 pairs of primers based on these sequences and used them to determine the polymorphisms between the stay-green cultivars (CN12, CN17, and CN18) and the control cultivar MY11. Among the 19 pairs of primers, 5 pairs produced polymorphisms between the stay-green cultivar and the non-stay-green control. Further studies of Chinese Spring nullisomic-tetrasomics show that JK738991 is mapped to 3B, JK738983 is mapped to 5D, and JK738989 is mapped to 2A, 4A, and 3D. The other two ESTs, JK738994 and JK739003, were not assigned to a chromosome using the Chinese Spring nullisomic-tetrasomics, which indicates that these ESTs may be derived from rye DNA in the wide cross. In particular, the ESTs that produce polymorphisms are notably useful in identifying the stay-green cultivar using molecular marker-assisted selection. The results also suggest that the in silico mapping data, even from a comparison genomic analysis based on the homogeneous comparison, are useful at some points, but the data were not always reliable, which requires further investigation using experimental methods.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
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ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)