Cereal Research Communications
Volume/Issue:
Volume 43: Issue 3
Sensitive detection of aflatoxin B1 molecules on gold SPR chip surface using functionalized gold nanoparticles
Authors:
A. Majzik University of Szeged, Dóm tér 8, H-6720 Szeged, Hungary

Search for other papers by A. Majzik in
Current site
Google Scholar
PubMed Close
,
V. Hornok University of Szeged, Dóm tér 8, H-6720 Szeged, Hungary

Search for other papers by V. Hornok in
Current site
Google Scholar
PubMed Close
,
D. Sebők University of Szeged, Dóm tér 8, H-6720 Szeged, Hungary

Search for other papers by D. Sebők in
Current site
Google Scholar
PubMed Close
,
T. Bartók University of Szeged, Moszkvai krt. 5–7, H-6725 Szeged, Hungary

Search for other papers by T. Bartók in
Current site
Google Scholar
PubMed Close
,
L. Szente CycloLab Ltd,, Illatos út 7, H-1097 Budapest, Hungary

Search for other papers by L. Szente in
Current site
Google Scholar
PubMed Close
,
K. Tuza CycloLab Ltd,, Illatos út 7, H-1097 Budapest, Hungary

Search for other papers by K. Tuza in
Current site
Google Scholar
PubMed Close
, and
I. Dékány University of Szeged, Dóm tér 8, H-6720 Szeged, Hungary
University of Szeged, Dóm tér 8, H-6720 Szeged, Hungary

Search for other papers by I. Dékány in
Current site
Google Scholar
PubMed Close
Pages:
426–437
Online Publication Date:
Sep 2015
Publication Date:
01 Sep 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/0806.43.2015.3.8
Keywords:
aflatoxin; gold nanoparticles; cyclodextrin; biosensor; SPR
Restricted access

Due to the warm and favourably humid climate of Southern Hungary, the maize is one of the most important crops. The protection against crop damage caused by fusarium and Aspergillus species is essential. Detection of aflatoxin B1 (AFB1) molecules in cereal crops by selective sensors is important, while they can cause serious diseases in humans and animals if they enter the food chain. Our main objective was to develop selective AFB1 sensor with increased sensitivity applying βCD-functionalized gold nanoparticles (AuβCD NPs) in surface plasmon resonance (SPR) measuring apparatus. The nanoparticles ca. 10 nm in diameter were prepared in the presence of thiol-modified cyclodextrin. The adsorption isotherms of AFB1 on bare, thiol-modified cyclodextrin and AuβCD NPs covered Au film surface were calculated using SPR platform. The AFB1 concentration can be quantitatively determined in the 0.001–23.68 ng/mL range. The AuβCD NPs were found to be highly sensitive and exhibited a remarkably low limit of detection (LOD; 1 pg/mL) without using other analytical reagents.

  • ChemAxon 2013. Marvin 6.0.0. http://www.chemaxon.com

  • Daly, S.J., Keating, G.J., Dillon, P.P., Manning, B.M., O’Kennedy, R., Lee, H.A., Morgan, M.R. 2000. Development of surface plasmon resonance-based immunoassay for aflatoxin B(1). Agric. Food Chem. 48:5097–5104.

    • Search Google Scholar
    • Export Citation

  • Du, Y., Luo, X.L., Xu, J.J., Chen, H.Y. 2007. A simple method to fabricate a chitosan-gold nps film and its application in glucose biosensor. Bioelectrochem. 70:342–347.

    • Search Google Scholar
    • Export Citation

  • Dunne, L., Daly, S., Baxter, A., Haughey, S., O’Kennedy, R. 2005. Surface plasmon resonance-based immunoassay for the detection of aflatoxin B1 using single-chain antibody fragments. Spectroscopy Letters 38:229–245.

    • Search Google Scholar
    • Export Citation

  • Feijter, J.A., Benjamins, J., Veer, F.A. 1978. Ellipsometry as a tool to study the adsorption behavior of synthetic and biopolymers at the air-water interface. Biopolymers 17:1759–1772.

    • Search Google Scholar
    • Export Citation

  • Ho, C., Robinson, A., Miller, D., Davis, M. 2005. Overview of sensors and needs for environmental monitoring. Sensors 5:4–37.

  • Hodnik, V., Anderluh, G. 2009. Toxin detection by surface plasmon resonance. Sensors 9:1339–1354.

  • Homola, J. 2008. Surface plasmon resonance sensors for detection of chemical and biological species. Chem. Review 108:462–493.

  • Huang, X., El-Sayed, I. H., Qian, W., El-Sayed, M. A. 2006. Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. JACS 128:2115–2120.

    • Search Google Scholar
    • Export Citation

  • Kham, K., Guerrouache, M., Carbonnier, B., Laterges, M., Perrot, H., Millot, M.C. 2007. Supramolecular interactions between b-cyclodextrin and hydrophobically end-capped poly(ethylene glycol)s: A quartz crystal microbalance study. J. Coll. Interf. Sci. 315: 800–804.

    • Search Google Scholar
    • Export Citation

  • Kim, J.H., Kim, K.S., Manesh, K.M., Santhosh, P., Gopalan, A.I., Lee, K.-P. 2008. Self-assembly directed synthesis of gold nanostructures. Colloids and Surfaces A 313–314:612–616.

    • Search Google Scholar
    • Export Citation

  • Li, P., Zhang, Q. 2009. Immunoassays for aflatoxins. Trends Analyt. Chem. 28:1115–1126.

  • Li, Y., Liu, X., Lin, Z. 2012. Recent developments and applications of surface plasmon resonance biosensors for the detection of mycotoxins is foodsuffs. Food Chem. 132:1549–1554.

    • Search Google Scholar
    • Export Citation

  • Liedberg, B., Lundström, I. 1993. Principles of biosensing with an extended coupling matrix and surface plasmon resonance. Sensors and Actuators A 11:63–72.

    • Search Google Scholar
    • Export Citation

  • Lu, Z., Chen, X., Wand, Y., Zheng, X., Li, C.M. 2015. Aptamer based fluorescence recovery assay for aflatoxin B1 using a quencher system composed of quantum dots and graphene oxide. Microchimica Acta 182:571–578.

    • Search Google Scholar
    • Export Citation

  • Luan, Y.X., Chen, Z.B., Xie, G., Chen, J.Y., Lu, A.X., Li, C., Fu, H.L., Ma, Z.H., Wang, J.H. 2015. Rapid visual detection of aflatoxin B1 by label-free aptasensor using unmodified gold nanoparticles. J. of Nanoscience and Nanotechnology 15:1357–1361.

    • Search Google Scholar
    • Export Citation

  • Mandal, A.K., Das, D.K., Das, A.K., Sen Mojumdar, S., Bhattacharyya, K. 2010. Study of gamma-cyclodextrin host-guest complex and nanotube aggregate by fluorescence correlation spectroscopy. J. Phys. Chem. B 115:10456–10461.

    • Search Google Scholar
    • Export Citation

  • Manetta, A.C., Di Giuseppe, L., Giammarco, M., Fusaro, I., Simonella, A., Gramenzi, A. 2005. Highperformance liquid chromatography with post-column derivatisation and fluorescence detection for sensitive determination of aflatoxin M1 in milk and cheese. J. Chromatography A. 1083:219–222.

    • Search Google Scholar
    • Export Citation

  • Moricz, A., Fater, Z., Otta, K.H., Tyihak, E., Mincsovics, E. 2007. Over pressured layer chromatographic determination of aflatoxin B1, B2, G1 and G2 in red paprika. Microch. J. 85:140–144.

    • Search Google Scholar
    • Export Citation

  • Moon, J., Kim, G., Lee, S. 2012. A gold nanoparticle and aflatoxin B1-BSA conjugates based lateral flow assay method for the analysis aflatoxin B1. Materials 5:634–643.

    • Search Google Scholar
    • Export Citation

  • Ogoshi, T., Harada, A. 2008. Chemical sensors based on cyclodextrin derivatives. Sensors 8:4961–4982.

  • Pál, L., Dublecz, K., Weber, M., Balogh, K., Erdélyi, M., Szigeti, G., Mézes, M. 2009. Effect of combined treatment with aflatoxin B1 and T-2 toxin and metabolites on some production traits and lipid peroxide status parameters of broiled chickens. Acta Veterinaria Hungarica 57:75–84.

    • Search Google Scholar
    • Export Citation

  • Peiwu, L., Qi, Z., Wen, Z., Jinyang, Z., Xiaomei, C., Jun, J., Lihua, X., Daohong, Z. 2009. Development of a class-specific monoclonal antibody-based ELISA for aflatoxins in peanut. Food Chem. 115:313–317.

    • Search Google Scholar
    • Export Citation

  • Perez-Juste, J., Pastoriza-Santos, I., Liz-Marzan, L.M., Mulvaney, P. 2005. Gold nanorods: Synthesis, characterization and applications. Coordination Chem. Review 249:1870–1901.

    • Search Google Scholar
    • Export Citation

  • Pestka, J.J., Gaur, P.K., Chu, F.S. 1980. Quantitation of aflatoxin B1 and aflatoxin B1 antibody by an enzymelinked immunosorbent microassay. Appl. Envir. Microb. 40:1027–1031.

    • Search Google Scholar
    • Export Citation

  • Sebők, D., Csapó, E., Preocanin, T., Bohus, G., Kallay, N., Dékány, I. 2013. Adsorption of ibuprofen and dopamine on functionalized gold using surface plasmon resonance spectroscopy at solid-liquid interface. Croatia Chemica Acta 86:287–295.

    • Search Google Scholar
    • Export Citation

  • Sharma, A., Matharu, Z., Sumana, G., Solanki, P.R., Kim, C.G., Malhotra, B.D. 2010. Antibody immobilized cysteamine functionalized-gold nanoparticles for aflatoxin detection. Thin Solid Films 519:1213–1218.

    • Search Google Scholar
    • Export Citation

  • Shim, W.-B., Mun, H., Joung, H.-A., Ofori, J.A., Chung, D.-H., Kim, M.-G. 2014. Chemoluminescence competitive aptamer assay for the detection of aflatoxin B1 in corn samples. Food Control 36:30–35.

    • Search Google Scholar
    • Export Citation

  • Soft Flow Hungary 2013. ELISA Kit for determination of total aflatoxin (B1, B2, G1, G2). Technical documentation 301013.

  • Szejtli, J. 1995. Selectivity/structure correlation in cyclodextrin chemistry. Supramolecular Chem. 6:217–223.

  • Szejtli, J. 2004. Past, present, and future of cyclodextrin research. Pure and Appl. Chem. 76:1825–1845.

  • Tumolo, T., Angnes, L., Baptista, M.S. 2004. Determination of the refractive index increment (dn/dc) of molecule and macromolecule solutions by surface plasmon resonance. Anal. Biochem. 333:273–279.

    • Search Google Scholar
    • Export Citation

  • Turkevich, J. 1985. Colloidal Gold. Part I. Gold Bulletin 18:86–91.

  • Urusov, A.E., Zherdev, A.V., Dzantiev, B.B. 2014a. Use of gold nanopatricle-labeled secondary antibodies to improve the sensitivity of an immunochromatographic assay for aflatoxin. Microchim Acta 181:1939–1946.

    • Search Google Scholar
    • Export Citation

  • Urusov, A.E., Petrakova, A.V., Vozniak, M.V., Zherdev, A.V., Dzantiev, B.B. 2014b. Rapid immunoenzyme assay of aflatoxin B1 using magnetic nanoparticles. Sensors 14:21843–21857.

    • Search Google Scholar
    • Export Citation

  • Yuan, J., Deng, D., Lauren, D.R., Aguilar, M.I., Wu, Y. 2009. Surface plasmon resonance biosensor for the detection of ochratoxin A in cereals and beverages. Analytica Chimica Acta 656:63–71.

    • Search Google Scholar
    • Export Citation

  • Var, I., Kabak, B., Gok, F. 2007. Survey of aflatoxin B1 in helva, a traditional Turkish food, by TLC. Food Control 18:59–62.

  • Varga, J., Frisvad, J.C., Samson, R.A. 2009. A reappraisal of fungi producing aflatoxins. World Mycotoxin J. 2:263–277.

  • Wang, X., Niessner, R., Knopp, D. 2014. Magnetic bead-based colorimetric immunoassay for aflatoxin B1 using gold nanoparticles. Sensors 14:21535–21548.

    • Search Google Scholar
    • Export Citation
  • Collapse
  • Expand
Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

 

 

For subscription options, please visit the website of Springer Nature.

Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2024 33 0 0
Sep 2024 38 0 0
Oct 2024 155 0 0
Nov 2024 189 0 0
Dec 2024 223 0 0
Jan 2025 62 0 0
Feb 2025 0 0 0