Comparative proteomic analysis of wheat developing grains between Chinese Spring and 1Sl/1B substitution line

Y. Liu; S. Wang; C. Wang; G. Chen; H. Cao; Y. Wang; W. Ma; Y. Hu; Y. Yan

doi:10.1556/0806.43.2015.040

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Cereal Research Communications

Volume/Issue:: Volume 44: Issue 1

Comparative proteomic analysis of wheat developing grains between Chinese Spring and 1S^l/1B substitution line

Authors:

Y. Liu

Y. Liu Capital Normal University, 100048 Beijing, China

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S. Wang

S. Wang Capital Normal University, 100048 Beijing, China
Murdoch University, A. Perth WA 6150, Australia

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C. Wang

C. Wang Capital Normal University, 100048 Beijing, China

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G. Chen

G. Chen Capital Normal University, 100048 Beijing, China

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H. Cao

H. Cao Capital Normal University, 100048 Beijing, China

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Y. Wang

Y. Wang Capital Normal University, 100048 Beijing, China

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W. Ma

W. Ma Murdoch University and Australian Export Grains Innovation Centre, Perth, WA 6150, Australia

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Y. Hu

Y. Hu Capital Normal University, 100048 Beijing, China

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Y. Yan

Y. Yan Capital Normal University, 100048 Beijing, China
Hubei Collaborative Innovation Center for Grain Industry (HCICGI), 434025 Jingzhou, China

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Pages:: 13–23

Online Publication Date:: Mar 2016

Publication Date:: 01 Mar 2016

Article Category:: Research Article

DOI:: https://doi.org/10.1556/0806.43.2015.040

Keywords:: wheat; differentially expressed proteins; 1Sl/1B substitution; comparative; proteome; expression patterns

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A comparative proteomic analysis of grain proteins during five grain developmental stages of wheat cultivar Chinese Spring (CS) and its 1S^l/1B substitution line CS-1S^l(1B) was carried out in the current study. A total of 78 differentially expressed protein (DEP) spots with at least 2-fold expression difference were detected by two-dimensional electrophoresis (2-DE). Among these, 73 protein spots representing 55 differentially expressed proteins (DEPs) were successfully identified by matrix-assisted laser desorption/ionization time-offlight tandem mass spectrometry (MALDI-TOF/TOF-MS). Differential protein spots between the two genotypes were analyzed by cluster software, which revealed significant proteome differences. There were 39 common spots (including 33 DEPs) that showed significant difference between the two lines across five grain developmental stages, of which 14 DEP spots (including 11 DEPs) were mainly involved in carbohydrate metabolism that were encoded by the genes on 1B chromosome while 25 DEP spots (including 12 DEPs) were mainly related to stress response and gluten quality that were encoded by 1S¹ chromosome. These results indicated that the S^l genome harbors more stress and quality related genes that are potential valuable for improving wheat stress resistance and product quality.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)