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A. Chumthong Songkhla Rajabhat University, Muang, Songkhla 90000, Thailand

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R. Wiwattanapatapee Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

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H. Viernstein University of Vienna, Althanstr.14, A-1090 Vienna, Austria

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A. Pengnoo Prince of Songkla University, HatYai, Songkhla 90112, Thailand

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M. Kanjanamaneesathian Silpakorn University, Phetchaburi IT campus, Cha-Am, Phetchaburi 76120, Thailand

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A spray-dried powder containing Bacillus megaterium was developed and tested for control of rice sheath blight disease in the greenhouse. The formulation consisted of 20 ml of an endospore suspension of B. megaterium, 20% w/v of skim milk powder and 1.25% w/v of polyvinyl pyrrolidone k-90, that were mixed and spray dried at 120 °C. The powder displayed good physical characteristics, such as a low-moisture content and a high solubility in water. Bacterial viability in the powder was 3.5±0.1 × 1011 cfu/g after production and remained relatively stable (at 2.2±0.1 × 1010 cfu/g) after 12 months of storage at room temperature. In the laboratory, a 0.1% (w/v) aqueous solution of the formulation was effective in inhibiting the mycelia growth of Rhizoctonia solani (98.5±0.1% inhibition). Under greenhouse conditions, a 0.1% (w/v) aqueous solution applied by either spraying 1 day before inoculating R. solani or spraying 1, 7 and 15 days after inoculation of rice plants with R. solani was more effective in suppressing sheath blight disease than the blank formulation but was less effective than a chemical fungicide control.

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  • Wiwattanapatapee, R., Chumthong, A., Pengnoo, A., Kanjanamaneesathian, M. 2007. Effervescent fast-disintegrating bacterial formulation for biological control of rice sheath blight. J. of Controlled Release 119:229235.

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  • Yánez-Mendizábal, V., Viñas, I., Usall, J., Torres, R., Solsona, C., Abadias, M., Teixidó, N. 2012. Formulation development of the biocontrol agent Bacillus subtilis strain CPA-8 by spray-drying. J. Appl. Microbiol. 112:954965.

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  • Zuberer, D.A. 1994. Recovery and enumeration of viable bacteria. In: Weaver, R.W., Angle, S., Bottomley, P., Bezdicek, D., Smith, S., Tabatabai, A., Wollum, A. (eds), Methods of Soil Analysis: Part 2, Microbiological and Biochemical Properties, Number 5. In Soil Science Society of America Book Series. Soil Science Soc. of America Inc. Wisconsin, USA. pp. 119143.

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Cereal Research Communications
Language English
Size A4
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)