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D.J. Liu
kaku2008@hotmail.com
D.J. Liu
Harbin Normal University, Harbin 150025, China
Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
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Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
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Y.B. Wang
Y.B. Wang
Harbin Normal University, Harbin 150025, China
Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
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Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
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C.H. Guo
C.H. Guo
Harbin Normal University, Harbin 150025, China
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Q. Cong
Q. Cong
Harbin Normal University, Harbin 150025, China
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X. Gong
X. Gong
Harbin Normal University, Harbin 150025, China
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H.J. Zhang
H.J. Zhang
Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
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Iron deficiency is the most common nutritional disorder, affecting over 30% of the world’s human population. The primary method used to alleviate this problem is nutrient biofortification of crops so as to improve the iron content and its availability in food sources. The over-expression of ferritin is an effective method to increase iron concentration in transgenic crops. For the research reported herein, sickle alfalfa (Medicago falcata L.) ferritin was transformed into wheat driven by the seed-storage protein glutelin GluB-1 gene promoter. The integration of ferritin into the wheat was assessed by PCR, RT-PCR and Western blotting. The concentration of certain minerals in the transgenic wheat grain was determined by inductively coupled plasma-atomic emission spectrometry, the results showed that grain Fe and Zn concentration of transgenic wheat increased by 73% and 44% compared to nontransformed wheat, respectively. However, grain Cu and Cd concentration of transgenic wheat grain decreased significantly in comparison with non-transformed wheat. The results suggest that the over-expression of sickle alfalfa ferritin, controlled by the seed-storage protein glutelin GluB-1 gene promoter, increases the grain Fe and Zn concentration, but also affects the homeostasis of other minerals in transgenic wheat grain.
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-
Amiri, R., Bahraminejad, S., Sasani, S., Jalali-Honarmand, S., Fakhri, R. 2015. Bread wheat genetic variation for grain’s protein, iron and zinc concentrations as uptake by their genetic ability. Eur. J. Agron. 67:20–26.
-
Borg, S., Brinch-Pedersen. H., Tauris, B., Madsen, L.H., Darbani, B., Noeparvar, S., Holm, P.B. 2012. Wheat ferritins: Improving the iron content of the wheat grain. J. Cereal Sci. 56:204–213.
-
Cui, S.P., Kang, Z.S., Jie, Z.H., Yu, X.M. 2006. A method of quickly extracting total RNA from wheat leaves. Acta Bot. Boreali-Occident. Sin. 26:314–318. (in Chinese)
-
Drakakaki, G., Christou, P., Stöger, E. 2000. Constitutive expression of soybean ferritin cDNA in transgenic wheat and rice results in increased iron levels in vegetative tissues but not in seeds. Transgenic Res. 9:445–452.
-
Drakakaki, G., Marcel, S., Glahn, R.P., Lund, E.K., Pariagh, S., Fischer, R., Christou, P., Stoger, E. 2005. Endosperm-specific co-expression of recombinant soybean ferritin and aspergillus phytase in maize results in significant increases in the levels of bioavailable iron. Plant Mol. Biol. 59:869–880.
-
Eagling, T., Neal, A.L., McGrath, S.P., Fairweather-Tait, S., Shewry, P.R., Zhao, F.J. 2014. Distribution and speciation of iron and zinc in grain of two wheat genotypes. J. Agric. Food Chem. 62:708–716.
-
Fernando, N., Panozzo, J., Tausz, M., Norton, R.M., Neumann, N., Fitzgerald, G.J. 2014. Elevated CO2 alters grain quality of two bread wheat cultivars grown under different environmental conditions. Agr. Ecosyst. Environ. 185:24–33.
-
Gomez-Galera, S., Rojas, E., Sudhakar, D., Zhu, C., Pelacho, A.M., Capell, T., Christou, P. 2010. Critical evaluation of strategies for mineral fortification of staple food crops. Transgenic Res. 19:165–180.
-
Goto, F., Yoshihara, T., Saiki, H. 1998. Iron accumulation in tobacco plants expressing soybean ferritin gene. Transgenic Res. 7:173–180.
-
Goto, F., Yoshihara, T., Saiki, H. 2000. Iron accumulation and enhanced growth in transgenic lettuce plants expressing the iron-binding protein ferritin. Theor. Appl. Genet. 100:658–664.
-
Goto, F., Yoshihara, T., Shigemoto, N., Toki, S., Takaiwa, F. 1999. Iron fortification of rice seed by the soybean ferritin gene. Nat. Biotechnol. 17:282–286.
-
Guo, C.H, Wang, Y.B., Li, L.Y., Sha, Y.Q. 2008. Cloning and sequence analyzing of ferritin cDNA from Medicago falcate L. J. of Harbin Institute of Technol. 41:141–145. (in Chinese)
-
Gupta, P.K., Mir, R.R., Mohan, A., Kumar, J. 2008. Wheat genomics: present status and future prospects. Int. J. Plant Genomics. Free PMC article. doi: 10.1155/2008/896451. pp. 1–36. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2397558/
-
Kanobe, M.N., Rodermel, S.R., Bailey, T., Scott, M.P. 2013. Changes in endogenous gene transcript and protein levels in maize plants expressing the soybean ferritin transgene. Front Plant Sci. 4:196.
-
Koorts, A.M., Viljoen, M. 2007. Ferritin and ferritin isoforms I: Structure–function relationships, synthesis, degradation and secretion. Arch. Physiol. Biochem. 113:30–54.
-
Kutman, U.B., Yildiz, B., Ozturk, L., Cakmak, I. 2010. Biofortification of durum wheat with zinc through soil and foliar applications of nitrogen. Cereal Chem. 87:1–9.
-
Liu, H., Wang, Z.H., Li, F., Li, K., Yang, N., Yang, Y. 2014. Grain iron and zinc concentrations of wheat and their relationships to yield in major wheat production areas in China. Field Crop Res. 156:151–160.
-
Lucca, P., Hurrell, R., Potrykus, I. 2001. Genetic engineering approaches to improve the bioavailability and the level of iron in rice grains. Theor. Appl. Genet. 102:392–397.
-
Lucca, P., Hurrell, R., Potrykus, I. 2002. Fighting iron deficiency anemia with iron-rich rice. J. Am. Coll. Cardiol. 21:184S–190S.
-
Masuda, H., Ishimaru, Y., Aung, M.S., Kobayashi, T., Kakei, Y., Takahashi, M., Higuchi, K., Nakanishi, H., Nishizawa, N.K. 2012. Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition. Sci. Rep. 2:543.
-
Masuda, H., Kobayashi, T., Ishimaru, Y., Takahashi, M., Aung, M.S., Nakanishi, H., Mori, S., Nishizawa, N.K. 2013. Iron-biofortification in rice by the introduction of three barley genes participated in mugineic acid biosynthesis with soybean ferritin gene. Front Plant Sci. 4:132.
-
Oury, F.X., Leenhardt, F., Rémésy, C., Chanliaud, E., Duperrier, B., Balfourier, F., Charmet, G., 2006. Genetic variability and stability of grain magnesium, zinc and iron concentrations in bread wheat. Eur. J. Agron. 25:177–185.
-
Ozturk, L., Yazici, M.A., Yucel, C., Torun, A., Cekic, C., Bagci, A., Ozkan, H., Braun, H.-J., Sayers, Z., Cakmak, I. 2006. Concentration and localization of zinc during seed development and germination in wheat. Physiologia Plantarum 128:144–152.
-
Paul, S., Ali, N., Gayen, D., Datta, S.K., Datta, K. 2012. Molecular breeding of Osfer 2 gene to increase iron nutrition in rice grain. GM Crops and Food 3:310–316.
-
Qu, L., Yoshihara, T., Ooyama, A. Goto, F. Takaiwa, F. 2005. Iron accumulation does not parallel the high expression level of ferritin in transgenic rice seeds. Planta 222:225–233.
-
Quan, J.L., He, Y.K., Chen, Y.f., Li, C.L., Guo, D.W., Han, D.J., Ling, Y. 2007. Establishment of efficient acceptor system for gene transformation by microprojectile bombardment in wheat. J. Northwest A&F Univ. (Natural Science Edition). 35:117–122. (in Chinese)
-
Regvar, M., Eichert, D., Kaulich, B., Gianoncelli, A., Pongrac, P., Vogel-Mikus, K., Kreft, I. 2011. New insights into globoids of protein storage vacuoles in wheat aleurone using synchrotron soft X-ray microscopy. J. Exp. Bot. 62:3929–3939.
-
Shi, R.l., Zou, C.Q., Rui, Y.K., Zhang, X.Y., Xia, X.P., Zhang, F.S. 2009. Application of ICP-AES to detecting nutrients in grain of wheat core collection of China. Spectroscopy and Spectral Analysis 29:1104–1107.
-
Shi, R.L., Tong, Y.P., Jing, R.L., Zhang, F.S., Zou, C.Q. 2013. Characterization of quantitative trait loci for grain minerals in hexaploid wheat (Triticum aestivum L.). J. Integr. Agr. 12:1512–1521.
-
Sui, X., Zhao, Y., Wang, S., Duan, X., Xu, L., Liang, R. 2012. Improvement Fe content of wheat (Triticum aestivum) grain by soybean ferritin expression cassette without vector backbone sequence. J. Agric. Biotechnol. 20:766–773. (in Chinese)
-
Syltie, P.W., Dahnke, W.C. 2005. Mineral and protein content, test weight and yield variations of hard spring wheat grain as influenced by fertilization and cultivar. Plant Food Human Nutri. 32:32–39.
-
Vasconcelos, M., Datta, K., Oliva, N., Khalekuzzaman, M., Torrizo, L., Krishnan, S., Oliveira, M., Goto, F., Datta, S.K. 2003. Enhanced iron and zinc accumulation in transgenic rice with the ferritin gene. Plant Sci. 164:371–378.
-
WHO 2014. Micronutrient deficiencies. http://www.who.int/nutrition/topics/ida/en/
-
Zhang, Y. , Wang, D.S., Zhang, Y., He, Z.H. 2007. Variation of major mineral elements concentration and their relationships in grain of Chinese wheat. Scientia Agricultura Sinica. 40:1871–1876.
-
Zhao, F.J., Su, Y.H., Dunham, S.J., Rakszegi, M., Bedo, Z., McGrath, S.P., Shewry, P.R., 2009. Variation in mineral micronutrient concentrations in grain of wheat lines of diverse origin. J. Cereal Sci. 49:290–295.
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| Cereal Research Communications | |
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