Authors:
J. Lu Shenyang Agricultural University, Shenyang 110866, China
Ministry of Agriculture, Tianjin 300191, China

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G.Z. Ji Shenyang Agricultural University, Shenyang 110866, China
Ministry of Agriculture, Tianjin 300191, China

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G. Li Ministry of Agriculture, Tianjin 300191, China
Tianjin Engineering Research Center of Agricultural Ecological & Environmental Remediation, Tianjin 300191, China

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Y.F. Wu Ministry of Agriculture, Tianjin 300191, China

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J. Yang Ministry of Agriculture, Tianjin 300191, China

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S.L. Lin Ministry of Agriculture, Tianjin 300191, China

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D.L. Yang Ministry of Agriculture, Tianjin 300191, China
Tianjin Engineering Research Center of Agricultural Ecological & Environmental Remediation, Tianjin 300191, China

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J.N. Zhao Shenyang Agricultural University, Shenyang 110866, China
Ministry of Agriculture, Tianjin 300191, China
Tianjin Engineering Research Center of Agricultural Ecological & Environmental Remediation, Tianjin 300191, China

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W.M. Xiu Shenyang Agricultural University, Shenyang 110866, China
Ministry of Agriculture, Tianjin 300191, China
Tianjin Engineering Research Center of Agricultural Ecological & Environmental Remediation, Tianjin 300191, China

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Global rice supplies have been found contaminated with unapproved varieties of genetically modified (GM) rice in recent years, which has led to product recalls in several of countries. Faster and more effective detection of GM contamination can prevent adulterated food, feed and seed from being consumed and grown, minimize the potential environmental, health or economic damage. In this study, a simple, reliable and cost-effective multiplex polymerase chain reaction (PCR) assay for identifying genetic modifications of TT51-1, Kemingdao1 (KMD1) and Kefeng6 (KF6) rice was developed by using the event-specific fragment. The limit of detection (LOD) for each event in the multiplex PCR is approximately 0.1%. Developed multiplex PCR assays can provide a rapid and simultaneous detection of GM rice.

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Cereal Research Communications
Language English
Size A4
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)