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  • 1 Shenyang Agricultural University, Shenyang 110866, China
  • | 2 Ministry of Agriculture, Tianjin 300191, China
  • | 3 Tianjin Engineering Research Center of Agricultural Ecological & Environmental Remediation, Tianjin 300191, China
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Global rice supplies have been found contaminated with unapproved varieties of genetically modified (GM) rice in recent years, which has led to product recalls in several of countries. Faster and more effective detection of GM contamination can prevent adulterated food, feed and seed from being consumed and grown, minimize the potential environmental, health or economic damage. In this study, a simple, reliable and cost-effective multiplex polymerase chain reaction (PCR) assay for identifying genetic modifications of TT51-1, Kemingdao1 (KMD1) and Kefeng6 (KF6) rice was developed by using the event-specific fragment. The limit of detection (LOD) for each event in the multiplex PCR is approximately 0.1%. Developed multiplex PCR assays can provide a rapid and simultaneous detection of GM rice.

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Cereal Research Communications
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