View More View Less
  • 1 Urmia University, Urmia, Iran
Restricted access

Drought is one of most important environmental factors inhibiting photosynthesis and decreasing growth and productivity of plants. The sensitivity of crop plants such as wheat to soil drought is particularly serious during reproductive phase is extremely sensitive to plant water status. The aim of this work was to study the effects of drought stress on photosynthesis, photosynthetic pigments, soluble proteins, a-tocopherol and abscisic acid content in six wheat genotypes, two tolerant (Daric and 92 Zhong), two moderately tolerant (Sabalan and DH-2049-3) and two sensitive (Shark and Tevee’s’). Total chlorophyll content, relative water content and chlorophyll a/b ratio decreased after long-time drought stress, that decrease in sensitive genotypes was higher than others. Net photosynthesis and stomatal conductance decreased significantly (P < 0.05) in flag leaves of our genotypes under drought stress after pollination, that decrease in sensitive genotypes was higher, too. Abscisic acid content, soluble protein content and a-tocopherol increased under drought stress, that increase in tolerant genotypes was higher. There was a significant negative correlation (P < 0.05) between photosynthesis factors and abscisic acid content in flag leaves of all genotypes. It can be concluded that Daric and 92 Zhong had a better photosynthesis factors compared to other genotypes and showed a higher capacity to tolerate drought stress.

  • Abdel-Nasser, L.E., Abdel-Aal, A.E. 2002. Effect of elevated CO2 and drought on proline metabolism and growth of safflower (Carthamus mareoticus L.) seedlings without improving water status. Pakist. J. Biol. Sci. 5:523528.

    • Search Google Scholar
    • Export Citation
  • Afzal, A., Gulzar, I., Shahbaz, M., Ashraf, M. 2014. Water deficit-induced regulation of growth, gas exchange, chlorophyll fluorescence, inorganic nutrient accumulation and antioxidative defense mechanism in mung bean [Vigna radiata (L.)Wilczek]. J. Appl. Bot. Food Qual. 87:147156.

    • Search Google Scholar
    • Export Citation
  • Agastian, P., Kingsley, S.J., Vivekanandan, M. 2000. Effect of salinity on photosynthesis and biochemical characteristics in mulberry genotypes. Photosynthetica 38:287290.

    • Search Google Scholar
    • Export Citation
  • Allahverdiyev, T. 2015. Effect of drought stress on some physiological traits of durum (Triticum durum Desf.) and bread (Triticum aestivum L.) wheat genotypes. J. Stress Physiol. Biochem. 11:2938.

    • Search Google Scholar
    • Export Citation
  • Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts, polyphennoloxidase in Beta vulgaris. Plant Physiol. 24:115.

  • Ashraf, M., Harris, P.J.C. 2013. Photosynthesis under stressful environments: An overview. Photosynthetica 51:163190.

  • Azhand, M., Saeidi, M., Abdoli, M. 2015. Evaluation of the relationship between gas exchange variables with grain yield in barley genotypes under terminal drought stress. Int. J. Biosci. 6:366374.

    • Search Google Scholar
    • Export Citation
  • Bradford, M.M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248254.

    • Search Google Scholar
    • Export Citation
  • Ciscato, M., Valcke, R., Van Loven, K., Clijsters, H., Navari-Izzo, F. 1997. Effect of in vivo copper treatment on the photosynthetic apparatus of two Triticum durum cultivars with different stress sensitivity. Physiol. Plant. 100:901908.

    • Search Google Scholar
    • Export Citation
  • Fang, Z., Bouwkamp, J., Solomos, T. 1998. Chlorophyllase activities and chlorophyll degradation during leaf senescence in nonyellowing mutant and wild type of Phaseolus vulgaris L. J. Exp. Bot. 49:503510.

    • Search Google Scholar
    • Export Citation
  • Guan, X.K., Song, L., Wang, T.C., Turner, N.C., Li, F.M. 2015. Effect of drought on the gas exchange, chlorophyll fluorescence and yield of six different-era spring wheat cultivars. J. Agron. Crop Sci. 201:241320.

    • Search Google Scholar
    • Export Citation
  • Hussien, H.A., Salem, H., Mekki, B.E.D. 2015. Ascorbate-glutathione-a-tocopherol triad enhances antioxidant systems in cotton plants grown under drought stress. Int. J. Chem. Tech. Res. 8:14631472.

    • Search Google Scholar
    • Export Citation
  • Jiang, Y., Huang, B. 2002. Protein alterations in tall festcue in response to drought stress and abscisic acid. Crop Sci. 42:202207.

  • Kelen, M., Demiralay, E.C., Sen, S., Ozkan, G. 2004. Seperation of abscisic acid. Indole 3 acetic acid, gibberellic acid in 99 R and rose oil (Rasa damascene Mill.) by reverse phase chromatography. Ital. J. of Biochem. 28:603610.

    • Search Google Scholar
    • Export Citation
  • Lawlor, D.W. 2002. Limitation of photosynthesis in water-stressed leaves. Stomatal metabolism and the role of ATP. Ann. Bot. 89:871885.

    • Search Google Scholar
    • Export Citation
  • Lawlor, D.W., Cornic, G. 2002. Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environ. 25:275294.

    • Search Google Scholar
    • Export Citation
  • Monakhova, O.F., Chernyadev, I.I. 2002. Protective role of kartolin-4 in wheat plants exposed to soil drought. Appl. Environ. Microbiol. 38:373380.

    • Search Google Scholar
    • Export Citation
  • Munné-Bosch, S., Schwarz, K., Alegre, L. 1999. a-Tocopherol protection against drought-induced damage in Rosmarinus officinalis L. and Melissa officinalis L. Z. Naturforsch. 54:698703.

    • Search Google Scholar
    • Export Citation
  • Neslihan-Ozturk, Z., Talamél, V., Deyholos, M., Michalowski, C.B., Galbraith, D.W., Gozukirmizi, N., Tuberosa, R., Bohnert, H.J. 2002. Monitoring large-scale changes in transcript abundance in drought- and salt stressed barley. Plant Mol. Biol. 48:551573.

    • Search Google Scholar
    • Export Citation
  • Parida, A.K., Das, A.B., Mittra, B. 2003. Effects of NaCl stress on the structure, pigment complex composition and photosynthetic activity of mangrove Bruguiera parviflora chloroplasts. Photosynthetica 41:191200.

    • Search Google Scholar
    • Export Citation
  • Riccardi, F., Gazeau, P., Vienne, D.V., Zivy, M. 1998. Protein changes in responses to progressive water deficit in maize. Plant Physiol. 117:12531263.

    • Search Google Scholar
    • Export Citation
  • Rumelin, A., Humbert, T., Fauth, U. 2004. Determination of alpha-tocopherol in plasma by high performance liquid chromatography with fluorescence detection and stability of alpha-tocopherol under different conditions. Arzneimittel-Forschung-Drug Research 54:376381.

    • Search Google Scholar
    • Export Citation
  • Saeidi, M., Abdoli, M. 2015. Effect of drought stress during grain filling on yield and its components, gas exchange variables, and some physiological traits of wheat cultivars. J. Agr. Sci. Tech. 17:885898.

    • Search Google Scholar
    • Export Citation
  • Sairam, R.K., Srivastava, G.S. 2001. Water stress tolerance of wheat (Triticum aestivum L.): Variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes. J. Agron. Crop Sci. 186:6370.

    • Search Google Scholar
    • Export Citation
  • Selote, D.S., Khana-Chopra, R. 2004. Drought-induced spikelet sterility is associated with an inefficient antioxidant defense in rice plants. Physiol. Plant. 121:462467.

    • Search Google Scholar
    • Export Citation
  • Tabaeizadeh, Z. 1998. Drought-induced responses in plant cells. Inter. Rev. Cytology 182:193247.

  • Travaglia, C., Cohen, A.C., Reinoso, H., Castillo, C., Bottini, R. 2007. Exogenous abscisic acid increases carbohydrate accumulation and redistribution to the grains in wheat grown under field conditions of soil water restriction. J. Plant Growth Regul. 26:285289.

    • Search Google Scholar
    • Export Citation
  • Yang, J., Zhang, J., Wang, Z., Zhu, Q., Liu, L. 2001. Water deficit induced senescence and its relationship to the remobilization of pre-stored carbon in wheat during gain filling. J. of Agron. 93:196206.

    • Search Google Scholar
    • Export Citation
  • Yang, J., Zhang, J., Wang, Z., Zhu, Q., Liu, L. 2002. Abscisic acid and cytokinins in the root exudates and leaves and their relationship to senescence and remobilization of carbon reserves in rice subjected to water stress during grain filling. Planta 215:645652.

    • Search Google Scholar
    • Export Citation
  • Wahid, A., Rasul, E. 2005. Photosynthesis in leaf, stem, flower and fruit. In: Pessarakli, M. (ed.), Hand Book of Photosynthesis, 2nd Ed. CRC Press. Florida, USA. pp. 479497.

    • Search Google Scholar
    • Export Citation