View More View Less
  • 1 Institute of Plant Biology and Biotechnology, 45 Timiryazev Street, 050040, Almaty, Kazakhstan
  • 2 Kazakh National Agricultural University, 8 Abay Av., Almaty, Kazakhstan
  • 3 Kazakh Research Institute of Farming, Almalybak, Almaty reg., Kazakhstan
  • 4 International Maize and Wheat Improvement Center (CIMMYT),, Emek, Ankara, Turkey
  • 5 Cereal Research Non-Profit Ltd. Co., H-6726, Alsó kikötő sor 9, Szeged, Hungary
Restricted access

Leaf rust, caused by Puccinia recondita f. sp. tritici, is one of the major diseases of wheat in Kazakhstan. To effectively use leaf rust resistance genes (Lr), it is important for breeders to know the resistance genotype in current cultivars. In this study, 30 winter wheat entries grown and/or produced in Kazakhstan were investigated using molecular markers to determine the presence and absence of eight important Lr genes. Molecular screening of these genotypes showed contrasting differences in the frequencies of these genes. Among the 30 entries, 17 carried leaf rust resistance gene Lr1, six had Lr26 and Lr34, and Lr10 and Lr37 were found in three cultivars. Two single cultivars separately carried Lr19 and Lr68, while Lr9 was not detected in any genotypes in this study. Field evaluation demonstrated that two of the most frequent two genes (Lr1 and Lr26) to be ineffective. While Lr34 provided some protection, the remaining effective Lr genes were found only in few genotypes: Lr37 occurred in Kazakh genotypes L-1090 and Krasnovodapadskaya 210 and in the US cultivar Madsen; Lr19 and Lr68 were likely present only in Russian and Kazakh cultivars, Pallada and Yegemen, respectively. The highest resistance over three years of leaf rust testing was found in Kazakh cultivars, Karasay, Krasnovodapadskaya 210, L-1090, Arap and Yegmen, foreign cultivars Madsen, Pallada and the control Parula (Lr68). Data may assist breeders to incorporate effective Lr genes into new cultivars.

  • Bartoš, P., Ovesna, J., Hanzalova, A., Chrpova, J., Dumalasova, V., Skorpik, M., Sip, V. 2004. Presence of a translocation from Aegilops ventricosa in wheat cultivars registered in the Czech Republic. Czech J. Genet. Plant Breed. 40:3135.

    • Search Google Scholar
    • Export Citation
  • Feuillet, C., Messmer, M., Schachermayr, G., Keller, B. 1995. Genetic and physical characterization of the Lr1 leaf rust resistance locus in wheat (Triticum aestivum L.). Mol. Gen. Genet. 248:553562.

    • Search Google Scholar
    • Export Citation
  • Gultyaeva, E.I. 2012. Methods for the identification of genes for resistance of wheat to leaf rust using DNA markers and characteristics of effective Lr genes. Russian Academy of Agricultural Sciences: VIZR. Saint- Petersburg, Russia. 72 p.

    • Search Google Scholar
    • Export Citation
  • Helguera, M., Khan, I.A., Kolmer, J., Lijavetzky, D., Zhong-qi, L., Dubcovsky, J. 2003. PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Sci. 43:18391847.

    • Search Google Scholar
    • Export Citation
  • Herrera-Foessel, S.A., Singh, R.P., Huerta-Espino, J., Rosewarne, G.M., Periyannan, S.K., Viccar, L., Calvo-Salazar, V., Lan, C., Lagudah, E.S. 2012. Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor. Appl. Genet. 124:14751486.

    • Search Google Scholar
    • Export Citation
  • Koishybayev, M.K., Zhanarbekova, A.B., Kokhmetova, A.V., Rsaliev, Sh.S. 2010. Genetic study of wheat resistance to leaf rust. Bulletin of National Academy of Sciences of Kazakhstan 6:1015.

    • Search Google Scholar
    • Export Citation
  • Kokhmetova, A., Sapakhova, Z., Madenova, A., Yessenbekova, G. 2014. Identification of carriers of yellow Yr5, Yr10, Yr15 and leaf Lr26, Lr34 rust resistance genes under molecular screening of wheat entries. Biotechnology. Theory and Practice 1:7178.

    • Search Google Scholar
    • Export Citation
  • Kosman, E., Pardes, E., Anikster, Y., Manisterski, J., Yehuda, P.B., Szabo, L.J., Sharon, A. 2004. Genetic variation and virulence on Lr26 in Puccinia triticina. Phytopathol. 94:632640.

    • Search Google Scholar
    • Export Citation
  • Lagudah, E.S., McFadden, H., Singh, R.P., Huerta-Espino, J., Bariana, H.S., Spielmeyer, W. 2006. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor. Appl. Genet. 114:2130.

    • Search Google Scholar
    • Export Citation
  • Lillemo, M., Singh, R.P., William, M., Herrera-Foessel, S.A., Huerta Espino, J., German, S., Campos, P., Chaves, M., Madriaga, R., Xia, X.C., Liang, S.S., Liu, D., Li, Z.F., Lagudah, E.S. 2011. Multiple rust resistance and gene additivity in wheat: Lessons from multi-location case studies in the cultivars Parula and Saar. BGRI Technical Workshop. St. Paul, Minnesota, USA. pp. 111120.

    • Search Google Scholar
    • Export Citation
  • McIntosh, R.A., Wellings, C.R., Park, R.F. 1995. Wheat Rusts: An Atlas of Resistance Genes. CSIRO. Sydney, Australia. pp. 234237.

  • McIntosh, R.A., Dubcovsky, J., Rogers, J., Morris, C., Appels, R., Xia, X. 2010. Catalogue of gene symbols for wheat: 2010 supplement. http:www.shigen.nig.ac.jp/wheat/komugi/genes/macgene 2010

    • Search Google Scholar
    • Export Citation
  • Moreno-Sevilla, B., Baenziger, P.S., Peterson C.J., Graybosch, R.A., McVey, D.V. 1995. The 1BL/1RS translocation: Agronomic performance of F3-derived lines from a winter wheat cross. Crop Sci. 35:10511055.

    • Search Google Scholar
    • Export Citation
  • Pathan, A.K., Park, R.F. 2006. Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars. Euphytica 149:327342.

    • Search Google Scholar
    • Export Citation
  • Peterson, R.F., Campbell, A.B., Hannah, A.E. 1948. A diagrammatic scale for estimating rust intensity of leaves and stem of cereals. Can. J. Res. Sect. C 26:496500.

    • Search Google Scholar
    • Export Citation
  • Pretorius, Z.A. 1988. First report of virulence to wheat leaf rust resistance gene Lr26 in South Africa. Plant Dis. 72:175.

  • Prins, R., Groenewald, J.Z., Marais, G.F., Snape, J.W., Koebner, R.M.D. 2001. AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor. Appl. Genet. 103:618624.

    • Search Google Scholar
    • Export Citation
  • Riede, C.R., Anderson, J.A. 1996. Linkage of RFLP markers to an aluminum tolerance gene in wheat. Crop Sci. 36:905909.

  • Roelfs, A.P., Singh, R.P., Saari, E.E. 1992. Rust diseases of wheat: Concepts and methods of disease management. CIMMYT. Mexico, D.F., Mexico. 81 p.

    • Search Google Scholar
    • Export Citation
  • Rsaliev, A.S. 2009. Resistance of spring durum wheat varieties (Triticum durum Desf) to rust diseases. Ph.D thesis of a dissertation for the Degree of Candidate of Agricultural Sciences on specialty 06.01.05 Plant Breeding and Seed Growing. CopyLand. Almalybak, Kazakhstan. 25 p.

    • Search Google Scholar
    • Export Citation
  • Rsaliev, Sh.S., Sedlovskii, A.I., Rsaliev, A.S., Tileubaeva, Zh.S., Tyupina, L.N., Yessenbekova, G.T., Atishova, M.N., Agabayeva, A.Ch. 2011. Catalogue of wheat cultivars and samples with resistance genes to leaf rust. IP Volkova. Almaty, Kazakhstan. 100 p.

    • Search Google Scholar
    • Export Citation
  • Schachermayr, G., Siedler, H., Gale, M.D., Winzeler, H., Winzeler, M., Keller, B. 1994. Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor. Appl. Genet. 88:110115.

    • Search Google Scholar
    • Export Citation
  • Schachermayr, G., Feuillet, C., Keller, B. 1997. Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds. Mol. Breeding 3:6574.

    • Search Google Scholar
    • Export Citation
  • Singh, R.P., Huerta-Espino, J., Rajaram, S., Crossa, J. 1998. Agronomic effects from chromosome translocations 7DL.7 Ag and 1BL.1RS in spring wheat. Crop Sci. 38:2733.

    • Search Google Scholar
    • Export Citation
  • Stubbs, R.W., Prescott, E.E., Saari, E.E., Dubin, H.J. 1986. Cereal disease methodology manual. CIMMYT. Mexico D.F., Mexico. 46 p.

  • Viuchkov, A.A. 1998. Breeding of spring bread and durum wheat in Middle Povoloje. Thesis of dissertation for the degree of Doctor Agricultural Sciences: 06.01.05. Bezenchuk, Russia. 66 p.

    • Search Google Scholar
    • Export Citation
  • Weng, Y., Azhaguvel, P., Devkota, R.N., Rudd, J.C. 2007. PCR-based markers for detection of different sources of 1AL.1RS and 1BL.1RS wheat-rye translocation in wheat background. Plant Breed. 126:482486.

    • Search Google Scholar
    • Export Citation