The mixograph performs certain rheological measurements during dough mixing and is a good predictor of wheat end-use quality. The aim of this study was to determine the expression and the heritability of mixing characteristics measured with Mixsmart® software and some quality characteristics in hard red spring wheat parents and their F1 progeny. Six parents varying in midline peak time and envelope peak time were crossed in a half diallel design. Parents and progeny were planted in three different environments. General combining ability (GCA) was a significant source of variation for the measured characteristics, and parents differed widely in terms of GCA effects. Midline-development time, -peak integral and -peak time showed high narrow sense heritability. Envelope peak-integral and -tail width displayed high narrow sense heritability for some, but not all locations. High GCA:SCA (specific combining ability) ratios indicated the prevalence of additive gene effects for midline-development time, -peak integral and -peak time, indicating that these characteristics are largely genetically determined, and that selection for them should lead to genetic gain.