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  • 1 Dokuz Eylul University, Buca, 35390, Izmir, Turkey
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The potential effect of combined salicylic acid and fish flour to improve plant tolerance to salt stress was investigated. This pre-treatment improved the growth of wheat seedlings under salinity when compared to control (untreated wheat seedlings). Moreover, combined pre-treatment improved significantly phenylalanine ammonia lyase (PAL) and peroxidase (POD) enzyme activities, also phenolic-flavonoid content in the shoots of salt stressed seedlings. One of the most important consequences of increase in salt stress is the oxidative tissue damage. In our study, salt stress increased lipid peroxidation levels (LPO) and also the loss of chlorophylls levels during stress might also be related to photo-oxidation resulting from oxidative stress. Whereas phenylalanine ammonia-lyase (PAL) activities of wheat shoots increased by a 2.1-fold under salt stress, the activities of shoots grown from seeds primed with salicylic acid and fish flour (SA + FF) increased by a 4-fold for 0.05 mM SA + FF, 4.8- fold for 0.1 mM SA + FF and 3.7-fold for 2.5 mM SA + FF combined pre-treatment under salt stress. Also, the combined salicylic acid + fish flour primed seedlings showed higher content of the scopoletin, and salicylic, syringic, vanilic and gallic acids under both salt and nonsalinity stress conditions.

  • Anonymus 2000. Fisheries statistics. State Institute of Statistics, Prime Ministry Republic of Turkey. Ankara, Turkey.

  • Arora, A., Byrem, T.M., Nair, M.G., Strasburg, G.M., 2000. Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Arch. Biochem. Biophys. 373:102109.

    • Search Google Scholar
    • Export Citation
  • Azooz, M.M. 2009. Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes in salt tolerance. Inter. J. Agric. Biol. 11:343350.

    • Search Google Scholar
    • Export Citation
  • Boscolo, W.R. 2001. Desempenhoe caracteristicas de carcaqa de machos revertidos de tilapias do Nilo (Oreochromis niloticus L.) (Performance and carcass characteristics of Nile Tilapia (Oreochromis niloticus L.) fed with rations containing different levels of fat). Revista Brasileire Zootecnol. 30:13911396. (in Spanish)

    • Search Google Scholar
    • Export Citation
  • Bradford, K.J. 1976. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortSci. 21:11051112.

    • Search Google Scholar
    • Export Citation
  • Buege, J.A., Aust, S.D. 1978. Microsomal lipid peroxidation. Methods Enzymol. 52:302310.

  • Dincer, T., Cakli, S., Kilinc, B., Tolasa, S. 2010. Amino acids and fatty acid composition content of fish sauce. J. Animal Vet. Advan. 9:311315.

    • Search Google Scholar
    • Export Citation
  • Du, L., Ali, G.S., Simons, K.A., Hou, J., Yang, T., Reddy, A.S., Poovanah, B.W. 2009. Ca2+/calmodulin regulates salicylic-acid-mediated plant immunity. Nature 457:11541158.

    • Search Google Scholar
    • Export Citation
  • Hodgins, D.S. 1971. Yeast phenylalanine ammonia-lyase. Purification, properties, and the identification of catalytically essential dehydroalanine. J. Biol. Chem. 46:29772985.

    • Search Google Scholar
    • Export Citation
  • Horii, A., Mccue, P., Shetty, K. 2007. Enhancement of seed vigour following insecticide and phenolic elicitor treatment. Biores. Technol. 98:623632.

    • Search Google Scholar
    • Export Citation
  • Iyengar, E.R.R., Reddy, M.P. 1996. Photosynthesis in highly salt tolerant plants. In: Pesserkali, M. (ed.), Handbook of Photosynthesis. Marshal Dekar. Baten Rose, USA. pp. 897909.

    • Search Google Scholar
    • Export Citation
  • Lan, T., You, J., Kong, L., Yu, M., Liu, M., Yang, Z. 2016. The interaction of salicylic acid and Ca alleviates aluminium toxicity in soybean. Plant Physiol. Biochem. 98:146154.

    • Search Google Scholar
    • Export Citation
  • Lee, J., Scagel, C.F. 2009. Chicoric acid found in basil (Ocimum basilicum L.) leaves. Food Chem. 115:650656.

  • Lichtenthaler, H.K. Wellburn, A.R. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 11:591592.

    • Search Google Scholar
    • Export Citation
  • Manaa, A., Gharbi, E., Mimouni, H., Wasti, S., Aschi-Smiti, S., Lutts, S., Ben Ahmed, H. 2014. Simultaneous application of salicylic acid and calcium improves salt tolerance in two contrasting tomato cultivars. South Afric. J. Bot. 95:3239.

    • Search Google Scholar
    • Export Citation
  • McCue, P., Zheng, Z., Pinkham, J.L., Shetty, K. 2000. A model for enhanced pea seedling vigour following low pH and salicylic acid treatments. Process Biochem. 35:600613.

    • Search Google Scholar
    • Export Citation
  • Miladinov, Z.J., Balesevic-Tubic, S.N., Ðordevic, V.B., Ðukic, V.H., Ilic, A.D., Cobanovic, L.M. 2015. Optimal time of soybean seed priming and primer effect under salt stress conditions. J. Agric. Sci. Belgrad 60:109117.

    • Search Google Scholar
    • Export Citation
  • Milic, B.L., Djilas, S.M., Canadanovic-Brunet, J.M. 1998. Antioxidative activity of phenolic compounds. Food Chem. 61:443447.

  • Nakano, Y., Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22:867880.

    • Search Google Scholar
    • Export Citation
  • Naz, F., Gul, H., Hamayun, M., Sayyed, A., Khan, H., Sherwani, S. 2014. Effect of NaCl stress on P, sativum germination and seedling growth with the influence ofseed priming with potassium (KCL and KOH). Am.- Eur. J. Agric. Environ. Sci. 14:13041311.

    • Search Google Scholar
    • Export Citation
  • Randhir, R., Shetty, K. 2003. Light-mediated fava bean (Vicia faba) response to phytochemical and protein elicitors and consequences on nutraceutical enhancement and seed vigour. Process Biochem. 38:945952.

    • Search Google Scholar
    • Export Citation
  • Saleh, A.M., Madany, M.M.Y. 2015. Coumarin pretreatment alleviates salinity stress in wheat seedlings. Plant Physiol. Biochem. 88:2735.

    • Search Google Scholar
    • Export Citation
  • Shah, Y., Klessig, D.F. 1999. Salicylic acid: signal perception and transduction. In: Hooykaas, P.J.J., Hall, M.A., Libbenga, K.R. (eds), Biochemistry and Molecular Biology of Plant Hormones. Elsevier Science Publications. Amsterdam, The Netherlands. pp. 513541.

    • Search Google Scholar
    • Export Citation
  • Shetty, K. 1997. Biotechnology to harness the benefits of dietary phenolics; focus on Lamiaceae. Asia Pacific. J. Clin. Nutr. 6:162171.

    • Search Google Scholar
    • Export Citation
  • Shetty, K., Randhir, R. 2005. Developmental stimulation of total phenolics and related antioxidant activity in light and dark germinated corn by natural elicitors. Process Biochem. 40:17211732.

    • Search Google Scholar
    • Export Citation
  • Tabatabaei, S.A. 2014. The effect of priming on germination indexes and seed reserve utilization of maize seeds under salinity stress. J. Seed Sci. Technol. 3:4451.

    • Search Google Scholar
    • Export Citation
  • Ventura, L., Dona, M., Macovei, A., Carbonera, D., Buttafava, A., Mondoni, A., Rossi, G., Balestrazzi, A. 2012. Understanding the molecular pathways associated with seed vigour. Plant Physiol. Biochem. 60:196206.

    • Search Google Scholar
    • Export Citation
  • Verstraeten, S.V., Keen, C.L., Schmitz, H.H., Fraga, C.G., Oteiza, P.L. 2003. Flavanols and procyanidins protect liposomes against lipid oxidation and disruption of the bilayer structure. Free Radic. Biol. Med. 34:8492.

    • Search Google Scholar
    • Export Citation
  • Waseem, M., Athar, H.U.R., Ashraf, M. 2006. Effect of salicylic acid applied through rooting medium on drought tolerance of wheat. Pakistan J. Bot. 38:11271136.

    • Search Google Scholar
    • Export Citation