Langdon(Dic-3A)-10 line carrying the QTL Qfhs.ndsu-3AS from T. turgidum ssp. dicoccoides that confers Type II resistance to Fusarium head blight (FHB) was crossed with Argentinean durum wheat cultivars. F4 progeny were screened with the microsatellite locus Xgwm2, tightly linked to the Qfhs.ndsu-3A region. Reaction of these plants and parents to FHB was evaluated at 7, 14 and 21 days post-inoculation (dpi) with F. graminearum; severity (% symptomatic spikelets/spike) and AUDPC (area under disease progress curve) were calculated. F4 progeny carrying the resistance allele in heterozygous or in homozygous condition showed significantly lower scab damage at 21 dpi and slower progress of disease than cultivated parents. Our results indicate that the resistance Qfhs.ndsu-3AS has a stable dominance expression in genetic backgrounds of durum cultivars and demonstrate that the linked microsatellite is an effective molecular tool for resistance screening. This work offers valuable information for Qfhs.ndsu-3AS utilization in wheat breeding programs.
Audenaert, K., Vanheule, A., Höfte, M., Haesaert, G. 2013. Deoxynivalenol: a major player in the multifaceted response of Fusarium to its environment. Toxins 6:1–19.
Bai, G.H., Shaner, G.E. 1994. Wheat scab: perspective and control. Plant Dis. 78:760–766.
Bai, G.H., Shaner, G.E., Ohm, H.W. 2000. Inheritance of resistance to Fusarium graminearum in wheat. Theor. Appl. Genet. 100:1–8.
Bai, G.H., Chen, L.F., Shaner, G. 2003. Breeding for resistance to Fusarium head blight of wheat in China. In: Leonard, K.J., Bushnell, W.R. (eds), Fusarium Head Blight of Wheat and Barley. APS Press, St. Paul, pp. 286–317.
Broders, K.D., Lipps, P.E., Paul, P.A., Dorrance, A.E. 2007. Evaluation of Fusarium graminearum associated with corn and soybean seed and seedling disease in Ohio. Plant Dis. 91:1155–1160.
Brown, N.A., Urban, M., Van de Meene, A.M., Hammond-Kosack, K.E. 2010. The infection biology of Fusarium graminearum: Defining the pathways of spikelet to spikelet colonisation in wheat ears. Fungal Biol. 114:555–571.
Buerstmayr, H., Ban, T., Anderson, J.A. 2009. QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breeding 128:1–26.
Buerstmayr, M., Huber, K., Heckmann, J., Steiner, B., Nelson, J.C., Buerstmayr, H. 2012. Mapping of QTL for Fusarium head blight resistance and morphological and developmental traits in three backcross populations derived from Triticum dicoccum and Triticum durum. Theor. Appl. Genet. 125:1751–1765.
Buerstmayr, M., Alimari, A., Steiner, B., Buerstmayr, H. 2013. Genetic mapping of QTL for resistance to Fusarium head blight spread (type 2 resistance) in a Triticum dicoccoides × Triticum durum backcrossderived population. Theor. Appl. Genet. 126:2825–2834.
Campbell, C.L., Madden, L.V. 1990. Introduction to Plant Disease Epidemiology. John Wiley & Sons, New York, USA. 532 pp.
Chakraborty, S., Newton, A.C. 2011. Climate change, plant diseases and food security: an overview. Plant Pathol. 60:2–14.
Chen, X., Faris, J.D., Hu, J., Stack, R.W., Adhikari, T., Elias, E.M., Kianian, S.F., Cai, X. 2007. Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat. Mol. Breeding 19:113–124.
Di Rienzo, J.A., Casanoves, F., Balzarini, M.G., Gonzalez, L., Tablada, M., Robledo, C.W. 2010. InfoStat, versión 2010, Grupo InfoStat. Available at: http://www.infostat.com.ar/.
Engle, J.S., Madden, L.V., Lipps, P.E. 2003. Evaluation of inoculation methods to determine resistance reactions of wheat to Fusarium graminearum. Plant Dis. 87:1530–1535.
Galich, M.T. 1997. Fusarium head blight in Argentina. In: Duvin, H.J., Gilchrist, L., Reeves, J., McNab, A. (eds) Fusarium Head Scab: Global Status and Future Prospects. Centro Internacional de Mejoramiento de Maiz y Trigo, Mexico, pp. 19–28.
Gilbert, J., Haber, S. 2013. Overview of some recent research developments in Fusarium head blight of wheat. Can. J. Plant Pathol. 35:149–174.
Huhn, M., Elias, E., Ghavami, F., Kianian, S., Chao, S., Zhong, S., Alamri, M., Yahyaoui, A., Mergoum, M. 2012. Tetraploid Tunisian wheat germplasm as a new source of Fusarium head blight resistance. Crop Sci. 52:136–145.
Jauhar, P.P., Peterson, T.S. 2008. Registration of DGE-1, a durum alien disomic addition line with resistance to Fusarium head blight. J. Plant Regist. 2:167–168.
Jauhar, P.P., Peterson, T.S., Xu, S.S. 2009. Cytogenetic and molecular characterization of durum alien disomic addition line with enhanced tolerance to Fusarium head blight resistance. Genome 52:467–483.
Jauhar, P.P., Peterson, T.S. 2012. Registration of DGE-2, a durum wheat disomic alien substitution line 1E(1A) involving a diploid wheatgrass chromosome. J. Plant Regist. 6:221–226.
Jauhar, P.P., Peterson, T.S. 2013. Registration of DGE-3, a durum wheat disomic substitution line 1E(1B) involving a wheatgrass chromosome. J. Plant Regist. 7:257–259.
Kang, Z., Buchenauer, H. 2000. Ultrastructural and immunocytochemical investigation of pathogen development and host responses in resistant and susceptible wheat spikes infected by Fusarium culmorum. Physiological and Molecular Plant Pathol. 57:255–268.
Kikot, G.E., Moschini, R., Consolo, V.F., Rojo, R., Salerno, G., Hours, R.A., Gasoni, L., Arambarri, A.M., Alconada, T.M. 2011. Occurrence of different species of Fusarium from wheat in relation to disease levels predicted by a weather-based model in Argentina pampas region. Mycopathologia 171:139–149.
Kosaka, A., Manickavelu, A., Kajihara, D., Nakagawa, H., Ban, T. 2015. Altered gene expression profiles of wheat genotypes against Fusarium head blight. Toxins 7:604–620.
Kumar, S., Stack, R.W., Friesen, T.L., Faris, J.D. 2007. Identification of a novel Fusarium head blight resistance quantitative trait locus on chromosome 7A in tetraploid wheat. Phytopathology 97:592–597.
Lemmens, M., Scholz, U., Berthiller, F., Dall’Asta, C., Koutnik, A., Schumacher, R., Adam, G., Buerstmayr, H., Mesterhazy, A., Krska, R., Ruckenbauer, P. 2005. The ability to detoxify the mycotoxin deoxynivalenol colocalizes with a major quantitative trait locus for Fusarium Head Blight resistance in wheat. Mol. Plant Microbe Interact. 18:1318–1324.
Martinez, M., Castañares, E., Dinolfo, M.I., Pacheco, W.G., Moreno, M.V., Stenglein, S.A. 2014. Presencia de Fusarium graminearum en muestras de trigo destinado al consumo humano. Rev. Argent. Microbiol. 46:41–44.
McMullen, M., Jones, R., Gallemberg, D. 1997. Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis. 81:1340–1348.
Miedaner, T., Horst Longin, C.F. 2014. Genetic variation for resistance to Fusarium head blight in winter durum material. Crop Pasture Sci. 65:46–51.
Miller, D.G., Gilmore, E.C., Manning, C.E. 1980. Cross pollination of wheat. J. Agron. Educ. 9:85–88.
Moschini, R.C., Galich, M.T.V., Annone, J.G., Polidoro, O. 2002. Enfoque fundamental-empírico para estimar la evolución del índice de Fusarium en Trigo. Rev. Invest. Agropecu. 31:39–53.
Moschini, R.C., Carranza, M.R., Carmona, M. 2004. Meteorological-based predictions of wheat head blight epidemic in the southern Argentinean pampas region. Cereal Res. Commun. 32:45–52.
Moschini, R.C., Martínez, M.I., Sepulcri, M.G. 2013. Modeling and forecasting systems for Fusarium head blight and deoxynivalenol content in wheat in Argentina. In: Alconada Magliano, T.M., Chulze, S.N. (eds), Fusarium Head Blight in Latin America. Editorial Springer, Netherlands, pp. 205–230.
Otto, C.D., Kianina, S.F., Elias, E.M., Stack, R.W., Joppa, L.R. 2002. Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat. Plant Mol. Biol. 48:625–632.
Parry, D.W., Jenkinson, P., McLeod, L. 1995. Fusarium ear blight (scab) in small grain cereals—a review. Plant Pathol. 44:207–238.
Prat, N., Buerstmayr, M., Steiner, B., Robert, O., Buerstmayr, H. 2014. Current knowledge on resistance to Fusarium Head Blight in tetraploid wheat. Mol. Breeding 34:1689–1699.
Röder, M.S., Korzun, V., Wandehake, K., Planschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149:2007–2023.
Ruan, Y., Comeau, A., Langevin, F., Hucl, P., Clarke, J.M., Brule-Babel, A., Pozniak, C.J. 2012. Identification of novel QTL for resistance to Fusarium head blight in a tetraploid wheat population. Genome 55:853–864.
Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, A.R., Allard, R.W. 1984. Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci. USA 81:8014–8018.
Schroeder, H.W., Christensen, J.J. 1963. Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838.
Seghezzo, M.L. 2015. Calidad en trigo candeal trigo candeal. Ediciones INTA, Buenos Aires, 64 pp.
Sissons, M. 2008. Role of durum wheat composition on the quality of pasta and bread. Food 2:75–90.
Somers, D.J., Fedak, G., Clarke, J., Cao, W. 2006. Mapping of FHB resistance QTLs in tetraploid wheat. Genome 49:1586–1593.
Soresi, D., Zappacosta, D., Garayalde, A.N., Miranda, R., Carrera, A.D. 2015. An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat. Phytopathol. Mediterr. 54:253–264.
Soresi, D., Carrera, A.D., Echenique, V., Garbus, I. 2015. Identification of genes induced by Fusarium graminearum inoculation in the resistant durum wheat line Langdon(Dic-3A)10 and the susceptible parental line Langdon. Microbiol. Res. 177:53–66.
Stack, R.W., Elias, E.M., Fetch, J.M., Miller, J.D., Joppa, L.R. 2002. Fusarium head blight reaction of Langdon durum Triticum dicoccoides chromosome substitution lines. Crop Sci. 42:637–642.
Talas, F., Longin, F., Miedaner, T. 2011. Sources of resistance to Fusarium head blight within Syrian durum wheat landraces. Plant Breeding 130:398–400.
Walter, S., Kahla, A., Arunachalam, C., Perochon, A., Khan, M.R., Scofield, S.R., Doohan, F.M. 2015. A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance. J. Exp. Bot. 66:2583–2593.
Wegulo, S.N. 2012. Factors influencing deoxynivalenol accumulation in small grain cereals. Toxins 4:1157–1180.
Zhang, Q., Axtman, J.E., Faris, J.D., Chao, S., Zhang, Z., Friesen, T.L., Zhong, S., Cai, X., Elias, E.M., Xu, S.S. 2014. Identification and molecular mapping of quantitative trait loci for Fusarium head blight resistance in emmer and durum wheat using a single nucleotide polymorphism-based linkage map. Mol. Breeding 34:1677–1687.
Zhu, X., Zhong, S., Chao, S., Gu, Y.Q., Kianian, S.F., Elias, E., Cai, X. 2015. Toward a better understanding of the genomic region harboring Fusarium head blight resistance QTL Qfhs.ndsu-3AS in durum wheat. Theor. Appl. Genet. 129:31–43.