TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

N. Li; Y. Zong; B.L. Liu; W.J. Chen; B. Zhang

doi:10.1556/0806.45.2017.027

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Cereal Research Communications

Volume/Issue:: Volume 45: Issue 3

TaMYB3, encoding a functional MYB transcriptor, isolated from the purple pericarp of Triticum aestivum

Authors:

N. Li

N. Li Chinese Academy of Sciences, Qinghai Xining 810008, China
Qinghai University, Qinghai Xining 810014, China
University of Chinese Academy of Sciences, Beijing 100049, China

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Y. Zong

Y. Zong Chinese Academy of Sciences, Qinghai Xining 810008, China
University of Chinese Academy of Sciences, Beijing 100049, China

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B.L. Liu

B.L. Liu Chinese Academy of Sciences, Qinghai Xining 810008, China
Qinghai Province Key Laboratory of Crop Molecular Breeding, Xining 810008, China

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W.J. Chen

W.J. Chen Chinese Academy of Sciences, Qinghai Xining 810008, China
Qinghai Province Key Laboratory of Crop Molecular Breeding, Xining 810008, China

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, and

B. Zhang

B. Zhang Chinese Academy of Sciences, Qinghai Xining 810008, China
Qinghai Province Key Laboratory of Crop Molecular Breeding, Xining 810008, China

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Pages:: 369–380

Online Publication Date:: Sep 2017

Publication Date:: 01 Sep 2017

Article Category:: Research Article

DOI:: https://doi.org/10.1556/0806.45.2017.027

Keywords:: MYB transcriptor; purple pericarp; anthocyanin biosynthesis; Triticum aestivum

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Purple pericarp is an interesting and useful trait in Triticum aestivum, but the molecular mechanism behind this phenotype remains unclear. The allelic variation in the MYB transcriptors is associated with the phenotype of pigmented organs in many plants. In this study, a MYB transcription factor gene, TaMYB3, was isolated using homology-based cloning and a differentially expressed gene mining approach, to verify the function of the MYB transcriptor in the purple pericarp. The coding sequence of TaMYB3 in cultivar Gy115 was the same as that in cultivar Opata. TaMYB3 was localized to FL0.62–0.95 on chromosome 4BL. The TaMYB3 protein contains DNA-binding and transcription-activation domains, and clustered on a phylogenetic tree with the MYB proteins that regulates anthocyanin and proanthocyanin biosynthesis. TaMYB3 localized in the nuclei of Arabidopsis thaliana and wheat protoplasts after it was transiently expressed with PEG transformation. TaMYB3 induced anthocyanin synthesis in the pericarp cells of Opata in the dark in collaboration with the basic helix–loop–helix protein ZmR, which is also the function of ZmC1. However, TaMYB3 alone did not induce anthocyanin biosynthesis in the pericarp cells of the white grain wheat cultivar Opata in the light after bombardment, whereas the single protein ZmR did. Light increased the expression of TaMYB3 in the pericarp of Gy115 and Opata, but only induced anthocyanin biosynthesis in the grains of Gy115. Our results extend our understanding of the molecular mechanism of the purple pericarp trait in T. aestivum.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)