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  • 1 Banaras Hindu University, Varanasi-Uttar Pradesh-221005, India
  • 2 Plant Genome Mapping Laboratory, 111 River Bend Road, University of Georgia, Athens, GA (USA)-30603
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Maize protein quality is deficit in essential amino acids, lysine and tryptophan. These constraints of o2 (opaque2) are corrected in genetically improved, hard endosperm QPM (Quality Protein Maize). An integrated strategy of phenotypic selection for endosperm modifiers and molecular marker-assisted foreground and background selection has been used in present study. The QPM donors were, CML 161, DMRQPM 58, CML 176 and CML 141 whereas, normal maize inbreds were CM 212, V338, V361, V336, V341, V351, CM 141 and V335. The inbreds were subjected to parental polymorphism survey between non-QPM and QPM using CIMMYT based three SSR markers, viz. phi057, umc1066 and phi112. Two markers, viz. phi057 and umc1066 exhibited co-dominant reactions, while phi112 was dominant in nature. Finally, two combinations V335 × CML 141 and V351 × CML 141 were considered for conversion program. Foreground selection was exercised using o2 specific marker umc1066 in BC1 and BC2 generations, while background as well as foreground selection was exercised in BC2F3 generation to recover the genome of recurrent parent up to extent of 80 to 100% with the help of SSR markers distributed across the whole maize genome. The tryptophan concentration in endosperm protein was significantly enhanced and the converted maize lines had almost twice the amount of lysine and tryptophan than normal maize inbreds.

  • Frisch, M., Bohn, M., Melchinger, A.E. 1999. Comparison of selection strategies for marker assisted backcrossing of a gene. Crop Sci. 39:12951301.

    • Search Google Scholar
    • Export Citation
  • Gupta, H.S., Babu, R., Agarwal, P.K., Mahajan, V., Hossian, F., Nepolean, T. 2014. Accelerated development of Quality Protein Maize hybrids through marker assisted introgression of opaque-2 allele. Plant Breed. 132:7782.

    • Search Google Scholar
    • Export Citation
  • Hernandez, H.H., Bates, L.S. 1969. A modified method for rapid tryptophan analysis in maize. CIMMYT Research Bulletin No. 13. CIMMYT. Mexico, D.F., Mexico.

    • Search Google Scholar
    • Export Citation
  • Hospital, F., Chevalet, C., Mulsant, P. 1992. Using markers in gene introgression programs. Genetics 132:11991210.

  • Krivanek, A., Groote, H., Gunaratna, N., Diallo, A., Freisen, D. 2007. Breeding and disseminating quality protein maize for Africa. Afr. J. Biotechnol. 6:312324.

    • Search Google Scholar
    • Export Citation
  • Lopes, M.A., Larkins, B.A. 1995. Genetic analysis of opaque2 modifier gene activity in maize endosperm. Theor. Appl. Genet. 119:274281.

    • Search Google Scholar
    • Export Citation
  • Mertz, E.T., Bates, L.S., Nelson, O.E. 1964. Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145:279280.

    • Search Google Scholar
    • Export Citation
  • Moro, G.L., Habben, J.E., Hamaker, B.R., Larkins, B.A. 1996. Characterization of the variability in lysine content for normal and opaque2 maize endosperm. Crop Sci. 36:16511659.

    • Search Google Scholar
    • Export Citation
  • Pixley, K.V., Bjarnasan, M.S. 1993. Combining ability for yield and protein quality among modified endosperm opaque 2 tropical maize inbreds. Crop Sci. 33:12291234.

    • Search Google Scholar
    • Export Citation
  • Prasanna, B.M., Vasal, S.K., Kasahun, B., Singh, N.N. 2001. Quality protein maize. Curr. Sci. 81:13081319.

  • Ram, L. 2014. Improvement of non QPM lines through introgression of QPM genes. PhD Thesis, BHU. Varanasi, India.

  • Ram, L., Singh, R., Singh, S.K. 2015. Study of combining ability using QPM donors as testers for yield and its component traits in maize (Zea mays L.). Sabrao J. Breeding and Genetics. 47:99112.

    • Search Google Scholar
    • Export Citation
  • Ribaut, J.M., Hoisington, D.A. 1998. Marker assisted selection: new tools and strategies. Trends Plant Sci. 3:236239.

  • Senior, M.L., Murphy, J.P., Goodman, M.M., Stuber, C.W. 1998. Utility of SSRs for determining genetic similarities and relationships in maize using an agarose gel system. Crop Sci. 38:10881098.

    • Search Google Scholar
    • Export Citation
  • Singh, R., Srivastava, R.P. 2015. New quantitative trait loci (QTLs) for turcicum leaf blight in maize. J. of Biotech. Crop Sci. 5:3543.

    • Search Google Scholar
    • Export Citation
  • Sofi, P.A., Wani, S.A., Rather, A.G., Wani, S.H. 2009. Quality protein maize (QPM): genetic manipulation for the nutritional fortification of maize. J. Plant Breed. Crop Sci. 1:244253.

    • Search Google Scholar
    • Export Citation
  • Tanksley, S.D. 1993. Mapping polygenes. Annu. Rev. Genet. 27:205233.

  • Vasal, S.K. 2001. High quality protein corn. In: Hallauer, A.R. (ed.), Specialty Corns. Second Edition. CRC Press. Boca Raton, FL, USA. pp. 85129.

    • Search Google Scholar
    • Export Citation
  • Villegas, E., Mertz, T.E. 1975. Simple chemical and biological methods used at Purdue University to evaluate cereals for protein quality. St. Bull 70:1721.

    • Search Google Scholar
    • Export Citation
  • Villegas, E., Ortega, E., Bauer, R. 1984. Chemical methods used at CIMMYT for determining protein quality in corn. CIMMYT. Mexico.

  • Visscher, P.M., Haley, C.S., Thompson, R. 1996. Marker assisted introgression in backcross breeding programs. Genetics 144:19231932.

  • Young, N.D., Tanksley, S.D. 1989. RFLP analysis of the size of chromosomal segments retained around “Tm-2 locus” of tomato during backcross breeding. Theor. Appl. Genet. 77:353359.

    • Search Google Scholar
    • Export Citation