Molecular cytogenetic identification of a wheat-Thinopyrum ponticum substitution line with stripe rust resistance

Q. Mo; C.Y. Wang; C.H. Chen; Y.J. Wang; H. Zhang; X.L. Liu; W.Q. Ji

doi:10.1556/0806.45.2017.037

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Cereal Research Communications

Volume/Issue:: Volume 45: Issue 4

Molecular cytogenetic identification of a wheat-Thinopyrum ponticum substitution line with stripe rust resistance

Authors:

Q. Mo

Q. Mo Northwest A&F University, Yangling, Shaanxi 712100, China

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C.Y. Wang

C.Y. Wang Northwest A&F University, Yangling, Shaanxi 712100, China

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C.H. Chen

C.H. Chen Northwest A&F University, Yangling, Shaanxi 712100, China

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Y.J. Wang

Y.J. Wang Northwest A&F University, Yangling, Shaanxi 712100, China

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H. Zhang

H. Zhang Northwest A&F University, Yangling, Shaanxi 712100, China

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X.L. Liu

X.L. Liu Northwest A&F University, Yangling, Shaanxi 712100, China

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W.Q. Ji

W.Q. Ji Northwest A&F University, Yangling, Shaanxi 712100, China

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Pages:: 564–573

Online Publication Date:: Dec 2017

Publication Date:: 01 Dec 2017

Article Category:: Research Article

DOI:: https://doi.org/10.1556/0806.45.2017.037

Keywords:: wheat; Thinopyrum ponticum ; disomic substitution line; stripe rust resistance; in situ hybridization; EST-STS markers

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Thinopyrum ponticum (2n = 10x = 70) has donated rust resistance genes to protect wheat from this fungal disease. In the present study, the line ES-7, derived from the progeny of the crosses between common wheat cultivar Abbondanza and Triticum aestivum–Th. ponticum partial amphiploid line Xiaoyan784, was characterized by cytological, fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and EST-STS marker techniques. Cytological observations revealed that the configuration of ES-7 was 2n = 42 = 21 II. GISH and FISH results showed that ES-7 had two St chromosomes and lacked 5A chromosomes compared to common wheat. The 4A chromosome of ES-7 had small alterations from common wheat. Two EST-SSR markers BE482522 and BG262826, specific to Th. ponticum and tetraploid Pseudoroegneria spicata (2n = 4x = 28), locate on the homoeologous group 5 chromosomes of wheat, could amplify polymorphic bands in ES-7. It was suggested that the introduced St chromosomes belonged to homoeologous group 5, that is, ES-7 was a 5St (5A) disomic substitution line. Furthermore, ES-7 showed highly resistance to mixed stripe rust races of CYR32 and CYR33 in adult stages, which was possibly inherited from Th. ponticum. Thus, ES-7 can be used for wheat stripe rust resistance breeding program.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)