Cereal Research Communications
Volume/Issue:
Volume 45: Issue 4
Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum
Authors:
Z.L. Li Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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H.Y. Li Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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G. Chen Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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X.J. Liu Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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C.L. Kou Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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S.Z. Ning Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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Z.W. Yuan Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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M. Hao Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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D.C. Liu Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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L.Q. Zhang Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

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Pages:
647–654
Online Publication Date:
Dec 2017
Publication Date:
01 Dec 2017
Article Category:
Research Article
DOI:
https://doi.org/10.1556/0806.45.2017.038
Keywords:
cultivated einkorn; glutenin; gene expression; quality breeding
Restricted access

Seven Glu-A1m allelic variants of the Glu-A1mx genes in Triticum monococcum ssp. monococcum, designated as 1Ax2.1a, 1Ax2.1b, 1Ax2.1c, 1Ax2.1d, 1Ax2.1e, 1Ax2.1f, and 1Ax2.1g were characterized. Their authenticity was confirmed by successful expression of the coding regions in E. coli, and except for the 1Ax2.1a with the presence of internal stop codons at position of 313 aa, all correspond to the subunit in seeds. However, all the active six genes had a same DNA size although their encoding subunits showed different molecular weight. Our study indicated that amino acid residue substitutions rather than previously frequently reported insertions/deletions played an important role on the subunit evolution of these Glu-A1mx alleles. Since variation in the Glu-A1x locus in common wheat is rare, these novel genes at the Glu-A1mx can be used as candidate genes for further wheat quality improvement.

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Cover Cereal Research Communications
Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company
Print ISSN:
0133-3720
Online ISSN:
1788-9170

 

 

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Cereal Research Communications
Language English
Size A4
Year of
Foundation		 1973
Volumes
per Year		 1
Issues
per Year		 4
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)

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