Novel D-hordein-like HMW glutenin sequences isolated from Psathyrostachys juncea by thermal asymmetric interlaced PCR

S.F. Dai; X.F. Xue; Y.F. Wang; Y.L. Xie; Z.P. Song; D.Y. Xu; Z.J. Wen; Z.H. Yan

doi:10.1556/0806.45.2017.060

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Cereal Research Communications

Volume/Issue:: Volume 46: Issue 1

Novel D-hordein-like HMW glutenin sequences isolated from Psathyrostachys juncea by thermal asymmetric interlaced PCR

Authors:

S.F. Dai

S.F. Dai Triticeae Research Institute, Sichuan Agricultural University, 611130, Wenjiang District, Chengdu, Sichuan, 611130, China

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X.F. Xue

X.F. Xue Triticeae Research Institute, Sichuan Agricultural University, 611130, Wenjiang District, Chengdu, Sichuan, 611130, China

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Y.F. Wang

Y.F. Wang Triticeae Research Institute, Sichuan Agricultural University, 611130, Wenjiang District, Chengdu, Sichuan, 611130, China

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Y.L. Xie

Y.L. Xie Triticeae Research Institute, Sichuan Agricultural University, 611130, Wenjiang District, Chengdu, Sichuan, 611130, China

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Z.P. Song

Z.P. Song Triticeae Research Institute, Sichuan Agricultural University, 611130, Wenjiang District, Chengdu, Sichuan, 611130, China

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D.Y. Xu

D.Y. Xu Triticeae Research Institute, Sichuan Agricultural University, 611130, Wenjiang District, Chengdu, Sichuan, 611130, China

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Z.J. Wen

Z.J. Wen Triticeae Research Institute, Sichuan Agricultural University, 611130, Wenjiang District, Chengdu, Sichuan, 611130, China

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Z.H. Yan

Z.H. Yan Triticeae Research Institute, Sichuan Agricultural University, 611130, Wenjiang District, Chengdu, Sichuan, 611130, China

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Pages:: 135–145

Online Publication Date:: Mar 2018

Publication Date:: 01 Mar 2018

Article Category:: Research Article

DOI:: https://doi.org/10.1556/0806.45.2017.060

Keywords:: Psathyrostachys juncea ; HMW glutenin; sequence analysis; thermal asymmetric interlaced PCR; phylogenetic analysis

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New high-molecular-weight glutenin (HMW glutenin) sequences isolated from six Psathyrostachys juncea accessions by thermal asymmetric interlaced PCR differ from previous sequences from this species. They showed novel modifications in all of the structural domains, with unique C-terminal residues, and their N-terminal lengths were the longest among the HMW glutenins reported to date. In their repetitive domains, there were three repeatable motif units: 13-residue [GYWH(/I/Y)YT(/Q)S(/T)VTSPQQ], hexapeptide (PGQGQQ), and tetrapeptide (ITVS). The 13-residue repeats were restricted to the current sequences, while the tetrapeptides were only shared by D-hordein and the current sequences. However, these sequences were not expressed as normal HMW glutenin proteins because an in-frame stop codon located in the C-termini interrupted the intact open reading frames. A phylogenetic analysis supported different origins of the P. juncea HMW glutenin sequences than that revealed by a previous study. The current sequences showed a close relationship with D-hordein but appeared to be more primitive.

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Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

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Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
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Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
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ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)