Authors:
G. Chen Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by G. Chen in
Current site
Google Scholar
PubMed
Close
,
M.H. Zhang Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by M.H. Zhang in
Current site
Google Scholar
PubMed
Close
,
X.J. Liu Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by X.J. Liu in
Current site
Google Scholar
PubMed
Close
,
J.Y. Fu Institute of Ecological Agriculture, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan 611130, China

Search for other papers by J.Y. Fu in
Current site
Google Scholar
PubMed
Close
,
H.Y. Li Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by H.Y. Li in
Current site
Google Scholar
PubMed
Close
,
M. Hao Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by M. Hao in
Current site
Google Scholar
PubMed
Close
,
S.Z. Ning Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by S.Z. Ning in
Current site
Google Scholar
PubMed
Close
,
Z.W. Yuan Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by Z.W. Yuan in
Current site
Google Scholar
PubMed
Close
,
Z.H. Yan Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by Z.H. Yan in
Current site
Google Scholar
PubMed
Close
,
B.H. Wu Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by B.H. Wu in
Current site
Google Scholar
PubMed
Close
,
D.C. Liu Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by D.C. Liu in
Current site
Google Scholar
PubMed
Close
, and
L.Q. Zhang Triticeae Research Institute, Sichuan Agricultural University at Chengdu, Wenjiang, Sichuan, 611130, China

Search for other papers by L.Q. Zhang in
Current site
Google Scholar
PubMed
Close
Restricted access

Premature termination codons (PTCs) are an important reason for the silence of highmolecular- weight glutenin subunits in Triticum species. Although the Glu-A1y gene is generally silent in common wheat, we here isolated an expressed Glu-A1y gene containing a PTC, named 1Ay8.3, from Triticum monococcum ssp. monococcum (AmAm, 2n = 2x = 14). Despite the presence of a PTC (TAG) at base pair positions 1879–1881 in the C-terminal coding region, this did not obviously affect 1Ay8.3 expression in seeds. This was demonstrated by the fact that when the PTC TAG of 1Ay8.3 was mutated to the CAG codon, the mutant in Escherichia coli bacterial cells expressed the same subunit as in the seeds. However, in E. coli, 1Ay8.3 containing the PTC expressed a truncated protein with faster electrophoretic mobility than that in seeds, suggesting that PTC translation termination suppression probably occurs in vivo (seeds) but not in vitro (E. coli). This may represent one of only a few reports on the PTC termination suppression phenomenon in genes.

Supplementary Materials

    • Supplementary Material
  • Collapse
  • Expand

 

 

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

 

 

For subscription options, please visit the website of Springer Nature.

Cereal Research Communications
Language English
Size A4
Year of
Foundation
1973
Volumes
per Year
1
Issues
per Year
4
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0133-3720 (Print)
ISSN 1788-9170 (Online)