Genetic characterisation and cytological identification of a male sterile mutant in maize (Zea mays L.)

G.C. Zhou; H.C. Shi; X.J. Yu; J.C. Yuan; Q. Guo; C.Y. Zhao; Q. Sun; Y.P. Ke

doi:10.1556/0806.46.2018.15

Jump to Content

Cereal Research Communications

Volume/Issue:: Volume 46: Issue 2

Genetic characterisation and cytological identification of a male sterile mutant in maize (Zea mays L.)

Authors:

G.C. Zhou

G.C. Zhou Sichuan Agricultural University, Chengdu, Sichuan 611130, China

Search for other papers by G.C. Zhou in
Current site
Google Scholar
PubMed

H.C. Shi

H.C. Shi Sichuan Agricultural University, Chengdu, Sichuan 611130, China

Search for other papers by H.C. Shi in
Current site
Google Scholar
PubMed

X.J. Yu

X.J. Yu Sichuan Agricultural University, Chengdu, Sichuan 611130, China

Search for other papers by X.J. Yu in
Current site
Google Scholar
PubMed

J.C. Yuan

J.C. Yuan Sichuan Agricultural University, Chengdu, Sichuan 611130, China

Search for other papers by J.C. Yuan in
Current site
Google Scholar
PubMed

Q. Guo

Q. Guo Sichuan Agricultural University, Chengdu, Sichuan 611130, China

Search for other papers by Q. Guo in
Current site
Google Scholar
PubMed

C.Y. Zhao

C.Y. Zhao Sichuan Agricultural University, Chengdu, Sichuan 611130, China

Search for other papers by C.Y. Zhao in
Current site
Google Scholar
PubMed

Q. Sun

Q. Sun Sichuan University, Chengdu, Sichuan 610064, China

Search for other papers by Q. Sun in
Current site
Google Scholar
PubMed

, and

Y.P. Ke

Y.P. Ke Sichuan Agricultural University, Chengdu, Sichuan 611130, China
Sichuan Nongda Zhenghong Bio. Co., Ltd, Chengdu, Sichuan 610213, China

Search for other papers by Y.P. Ke in
Current site
Google Scholar
PubMed

View More View Less

Pages:: 344–354

Online Publication Date:: Jun 2018

Publication Date:: 01 Jun 2018

Article Category:: Research Article

DOI:: https://doi.org/10.1556/0806.46.2018.15

Keywords:: maize; male sterile; genetic analysis; cytoplasmic identification; tapetum

Restricted access

Male sterile mutants play an important role in the utilisation of crop heterosis. Male sterile plants were found in S₅ generations of maize hybrid ZH2, through continuous sib-mating by using the fertile plants in the same population, we obtained a male sterile sibling population K932MS including sterile plants K932S and a fertile plant K932F. The objective of this study was to clarify the genetic characterisation and abortion characteristics by nucleus and cytoplasm effect analyses, cytoplasm grouping, and cytological observation. The results showed that no difference was found between K932S and K932F in the vegetative growth stage, but K932S had no emerging anther or pollen grains. The segregation ratio of fertile plants to sterile plants was 1:1 in the sibling progenies, while it was 3:1 in self-crossing progenies of K932F. The sterility of K932S could be restored among reciprocal progenies when seven normal inbred lines were used as females respectively. The fertility expression of K932S crossed with 30 testers would be changed in different test-crosses and some backcross progenies. The C-type restorer Zifeng-1 (Rf4Rf4) was able to restore the fertility of K932S, and the specific PCR amplification bands of K932MS were consistent with CMSC_Mo17. The anther of K932S began abortion at dyad with its tapetum expanded radically and vacuolated: this induced abnormality in the shapes of both dyads and tetrads. The microspore could not develop normally, and then it collapsed and gradually disappeared. Hence, K932MS is a C-type cytoplasmic male sterile mutant with a pollen-free, stable inheritance: it has potential application value for further research.

Supplementary Materials

Save
Cite
Email this content

Share Link

Copy this link, or click below to email it to a friend
Email this content
or copy the link directly:

https://akjournals.com/abstract/journals/0806/46/2/article-p344.xml
The link was not copied. Your current browser may not support copying via this button.

Link copied successfully

Collapse
Expand

Cereal Research Communications A Quarterly of the Cereal Research Non-Profit Ltd. Company

Print ISSN:: 0133-3720

Online ISSN:: 1788-9170

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Cereal Research Communications
Language	English
Size	A4
Year of Foundation	1973
Volumes per Year	1
Issues per Year	4
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0133-3720 (Print)
ISSN	1788-9170 (Online)